Publikationer pr. år
Publikationer pr. år
Thorvaldsensvej 40, 1871 Frederiksberg C
Publikationer pr. år
Languages of the Non-coding Genome
Genomes are the blueprints of life: the genomic DNA sequences contain the instructions for the formation of a given species. DNA sequence polymorphisms between individuals of the same species offer a hypothesis to understand the molecular bases of fitness differences. However, many polymorphisms are located in parts of the genome that we poorly understand: the non-coding genome. While polymorphisms that change the protein sequence offer a sufficiently clear path forward, my seminar outlines approaches and solutions to understand the non-coding genome.
Results from my laboratory illustrate different molecular mechanisms of how the non-coding genome promotes organismal fitness. We developed methods that reveal where the non-coding genome is transcribed by RNA polymerase II (Pol II) into RNAs. Here, a particular focus is on thousands of RNAs that are missed by many transcriptomics approaches even when located in important regions. While we find some cases of trans-acting RNAs, a common theme resulting from our functional characterization of specific non-coding loci is that the act of Pol II transcription explains the function of non-coding region, rather than the resulting RNA product. Essentially, chromatin-based signaling during Pol II transcription in the non-coding genome represses mRNA initiation when the transcription units overlap. Efforts to characterize non-coding RNA isoforms overlapping with genes involved a new class of non-coding RNAs: short promoter-proximal RNAs (sppRNAs). sppRNAs result from transcriptional termination at sites of promoter-proximal Pol II stalling and are positively correlated with gene expression. In conclusion, our results highlight different ways of how the non-coding genome communicates functional information, with intriguing implications for current models explaining non-coding genome conservation and function.
Biological Sciences, PhD, University of East Anglia
Dimissionsdato: 16 jul. 2010
Postdoc in non-coding transcription, postdoctoral researcher, Harvard Medical School
2010 → 2014
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Publikation: Bidrag til tidsskrift › Review › Forskning › peer review
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Publikation: Working paper › Preprint
Sebastian Marquardt (Andet)
Aktivitet: Tale eller præsentation - typer › Foredrag og mundtlige bidrag
Sebastian Marquardt (Deltager), Daniel Martin Messerschmidt (Arrangør), Edda Schulz (Arrangør), Melanie Hamon (Arrangør) & Alan Cheung (Arrangør)
Aktivitet: Deltagelse i arrangement eller begivenhed - typer › Organisation af og deltagelse i konference
Sebastian Marquardt (Andet)
Aktivitet: Tale eller præsentation - typer › Foredrag og mundtlige bidrag
Sebastian Marquardt (Andet)
Aktivitet: Tale eller præsentation - typer › Foredrag og mundtlige bidrag
Sebastian Marquardt (Andet)
Aktivitet: Tale eller præsentation - typer › Foredrag og mundtlige bidrag
Sebastian Marquardt (Vært)
Aktivitet: Vært for en besøgende - typer › Vært for en akademisk besøgende
Sebastian Marquardt (Deltager)
Aktivitet: Andre aktivitetstyper › Andet (priser, ekstern undervisning samt andet). - Andet
Sebastian Marquardt (Andet)
Aktivitet: Tale eller præsentation - typer › Foredrag og mundtlige bidrag
Marquardt, Sebastian (Modtager), 2015
Pris: Priser, stipendier, udnævnelser
Marquardt, Sebastian (Modtager), 2017
Pris: Priser, stipendier, udnævnelser
Marquardt, Sebastian (Modtager), 1 jan. 2020
Pris: Priser, stipendier, udnævnelser
Marquardt, Sebastian (Modtager), 2021
Pris: Priser, stipendier, udnævnelser