A human gut microbial gene catalogue established by metagenomic sequencing

MetaHIT Consortium

doi:10.1038/nature08821

A human gut microbial gene catalogue established by metagenomic sequencing

MetaHIT Consortium

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

9216 Citationer (Scopus)

1365 Downloads (Pure)

Abstract

Udgivelsesdato: 2010-Mar-4

Originalsprog	Engelsk
Tidsskrift	Nature
Vol/bind	464
Udgave nummer	7285
Sider (fra-til)	59-65
Antal sider	6
ISSN	0028-0836
DOI	https://doi.org/10.1038/nature08821
Status	Udgivet - 2010

Bibliografisk note

Keywords: Adult; Bacteria; Cohort Studies; Contig Mapping; Denmark; Feces; Gastrointestinal Tract; Genes, Bacterial; Genes, Essential; Genome, Bacterial; Genomics; Health; Humans; Inflammatory Bowel Diseases; Metagenome; Obesity; Open Reading Frames; Overweight; Sequence Analysis, DNA; Spain

Adgang til dokumentet

10.1038/nature08821Licens: CC BY-NC-SA

Qin_2010_A_human_gut_microbialForlagets udgivne version, 922 KBLicens: CC BY-NC-SA

Citationsformater

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title = "A human gut microbial gene catalogue established by metagenomic sequencing",

abstract = "To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.",

author = "Junjie Qin and Ruiqiang Li and Jeroen Raes and Manimozhiyan Arumugam and Burgdorf, {Kristoffer Solvsten} and Chaysavanh Manichanh and Trine Nielsen and Nicolas Pons and Florence Levenez and Takuji Yamada and Mende, {Daniel R.} and Junhua Li and Junming Xu and Shaochuan Li and Dongfang Li and Jianjun Cao and Bo Wang and Huiqing Liang and Huisong Zheng and Yinlong Xie and Julien Tap and Patricia Lepage and {dos Santos}, {Marcelo Bertalan Quintanilha} and Jean-Michel Batto and Torben Hansen and {Le Paslier}, Denis and Linneberg, {Allan Ren{\'e}} and Nielsen, {H. Bj{\o}rn} and Eric Pelletier and Pierre Renault and Thomas Sicheritz-Ponten and Keith Turner and Hongmei Zhu and Chang Yu and Shengting Li and Min Jian and Yan Zhou and Yingrui Li and Xiuqing Zhang and Songgang Li and Nan Qin and Huanming Yang and Jian Wang and S{\o}ren Brunak and Joel Dor{\'e} and Francisco Guarner and Karsten Kristiansen and Pedersen, {Oluf Borbye} and Julian Parkhill and {MetaHIT Consortium} and Jun Wang",

note = "Keywords: Adult; Bacteria; Cohort Studies; Contig Mapping; Denmark; Feces; Gastrointestinal Tract; Genes, Bacterial; Genes, Essential; Genome, Bacterial; Genomics; Health; Humans; Inflammatory Bowel Diseases; Metagenome; Obesity; Open Reading Frames; Overweight; Sequence Analysis, DNA; Spain",

year = "2010",

doi = "10.1038/nature08821",

language = "English",

volume = "464",

pages = "59--65",

journal = "Nature",

issn = "0028-0836",

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T1 - A human gut microbial gene catalogue established by metagenomic sequencing

AU - Qin, Junjie

AU - Li, Ruiqiang

AU - Raes, Jeroen

AU - Arumugam, Manimozhiyan

AU - Burgdorf, Kristoffer Solvsten

AU - Manichanh, Chaysavanh

AU - Nielsen, Trine

AU - Pons, Nicolas

AU - Levenez, Florence

AU - Yamada, Takuji

AU - Mende, Daniel R.

AU - Li, Junhua

AU - Xu, Junming

AU - Li, Shaochuan

AU - Li, Dongfang

AU - Cao, Jianjun

AU - Wang, Bo

AU - Liang, Huiqing

AU - Zheng, Huisong

AU - Xie, Yinlong

AU - Tap, Julien

AU - Lepage, Patricia

AU - dos Santos, Marcelo Bertalan Quintanilha

AU - Batto, Jean-Michel

AU - Hansen, Torben

AU - Le Paslier, Denis

AU - Linneberg, Allan René

AU - Nielsen, H. Bjørn

AU - Pelletier, Eric

AU - Renault, Pierre

AU - Sicheritz-Ponten, Thomas

AU - Turner, Keith

AU - Zhu, Hongmei

AU - Yu, Chang

AU - Li, Shengting

AU - Jian, Min

AU - Zhou, Yan

AU - Li, Yingrui

AU - Zhang, Xiuqing

AU - Li, Songgang

AU - Qin, Nan

AU - Yang, Huanming

AU - Wang, Jian

AU - Brunak, Søren

AU - Doré, Joel

AU - Guarner, Francisco

AU - Kristiansen, Karsten

AU - Pedersen, Oluf Borbye

AU - Parkhill, Julian

AU - MetaHIT Consortium

AU - Wang, Jun

N1 - Keywords: Adult; Bacteria; Cohort Studies; Contig Mapping; Denmark; Feces; Gastrointestinal Tract; Genes, Bacterial; Genes, Essential; Genome, Bacterial; Genomics; Health; Humans; Inflammatory Bowel Diseases; Metagenome; Obesity; Open Reading Frames; Overweight; Sequence Analysis, DNA; Spain

PY - 2010

Y1 - 2010

N2 - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.

AB - To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.

U2 - 10.1038/nature08821

DO - 10.1038/nature08821

M3 - Journal article

C2 - 20203603

SN - 0028-0836

VL - 464

SP - 59

EP - 65

JO - Nature

JF - Nature

IS - 7285

ER -