A hybrid receptor binding protein enables phage F341 infection of Campylobacter by binding to flagella and lipooligosaccharides

Line Jensen Ostenfeld, Anders Nørgaard Sørensen, Horst Neve, Amira Vitt, Jochen Klumpp, Martine Camilla Holst Sørensen*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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Abstract

Flagellotropic bacteriophages are interesting candidates as therapeutics against pathogenic bacteria dependent on flagellar motility for colonization and causing disease. Yet, phage resistance other than loss of motility has been scarcely studied. Here we developed a soft agar assay to study flagellotropic phage F341 resistance in motile Campylobacter jejuni. We found that phage adsorption was prevented by diverse genetic mutations in the lipooligosaccharides forming the secondary receptor of phage F341. Genome sequencing showed phage F341 belongs to the Fletchervirus genus otherwise comprising capsular-dependent C. jejuni phages. Interestingly, phage F341 encodes a hybrid receptor binding protein (RBP) predicted as a short tail fiber showing partial similarity to RBP1 encoded by capsular-dependent Fletchervirus, but with a receptor binding domain similar to tail fiber protein H of C. jejuni CJIE1 prophages. Thus, C. jejuni prophages may represent a genetic pool from where lytic Fletchervirus phages can acquire new traits like recognition of new receptors.

OriginalsprogEngelsk
Artikelnummer1358909
TidsskriftFrontiers in Microbiology
Vol/bind15
Antal sider14
ISSN1664-302X
DOI
StatusUdgivet - 2024

Bibliografisk note

Funding Information:
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The work presented here was supported by the Danish Council of Independent Research (Grant 4184-00109B), the Danish AgriFish Agency of Ministry of Environment and Food (Grant 34009-14-0873), and Intralytix, Inc. for financial support. We also acknowledge funding of JK by Martin J. Loessner, ETH Zurich.

Funding Information:
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. The work presented here was supported by the Danish Council of Independent Research (Grant 4184-00109B), the Danish AgriFish Agency of Ministry of Environment and Food (Grant 34009-14-0873), and Intralytix, Inc. for financial support. We also acknowledge funding of JK by Martin J. Loessner, ETH Zurich.

Publisher Copyright:
Copyright © 2024 Ostenfeld, Sørensen, Neve, Vitt, Klumpp and Sørensen.

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