Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Journal of Neurochemistry |
| Vol/bind | 63 |
| Udgave nummer | 5 |
| Sider (fra-til) | 1675-82 |
| Antal sider | 7 |
| ISSN | 1471-4159 |
| Status | Udgivet - 1994 |
| Udgivet eksternt | Ja |
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A kinetic analysis of 6-[18F]fluoro-L-dihydroxyphenylalanine metabolism in the rat. / Cumming, P; Kuwabara, H; Gjedde, A.
I: Journal of Neurochemistry, Bind 63, Nr. 5, 1994, s. 1675-82.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - A kinetic analysis of 6-[18F]fluoro-L-dihydroxyphenylalanine metabolism in the rat.
AU - Cumming, P
AU - Kuwabara, H
AU - Gjedde, A
PY - 1994
Y1 - 1994
N2 - A previous study of the metabolism of 6-[18F]-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) in rats pretreated with carbidopa contained information amenable to kinetic analysis. Using these data, tracer transfer coefficients and metabolic rate constants were estimated. After intravenous injection, FDOPA in circulation was O-methylated (k0D = 0.055 min-1), and the metabolite (O-methyl-FDOPA) escaped from plasma with a rate constant (k-1M) of 0.01 min-1. The initial clearance of FDOPA to striatum (K1D) was 0.07 ml g-1 min-1, and the equilibrium distribution volume (VeD) was 0.67 ml g-1. The initial clearance of O-methyl-FDOPA to striatum (K1M) was 0.08 ml g-1 min-1, and the equilibrium distribution volume (VeM) was 0.75 ml g-1. The rate constant of FDOPA decarboxylation (k3D) was 0.17 min-1 in striatum. The elimination of 6-[18F]fluorodopamine (FDA) from striatum suggested an apparent rate constant for monoamine oxidase activity (k7') of 0.055 min-1. 6-[18F]Fluorohomovanillic acid (FHVA) was formed from 6-[18F]fluoro-L-3,4-dihydroxyphenylacetic acid with a rate constant (k11) of 0.083 min-1, and FHVA was eliminated from striatum (k9) with a rate constant of 0.12 min-1. The steady-state concentration ratios of FDA and its metabolites were shown to be functions of these rate constants.
AB - A previous study of the metabolism of 6-[18F]-fluoro-L-3,4-dihydroxyphenylalanine (FDOPA) in rats pretreated with carbidopa contained information amenable to kinetic analysis. Using these data, tracer transfer coefficients and metabolic rate constants were estimated. After intravenous injection, FDOPA in circulation was O-methylated (k0D = 0.055 min-1), and the metabolite (O-methyl-FDOPA) escaped from plasma with a rate constant (k-1M) of 0.01 min-1. The initial clearance of FDOPA to striatum (K1D) was 0.07 ml g-1 min-1, and the equilibrium distribution volume (VeD) was 0.67 ml g-1. The initial clearance of O-methyl-FDOPA to striatum (K1M) was 0.08 ml g-1 min-1, and the equilibrium distribution volume (VeM) was 0.75 ml g-1. The rate constant of FDOPA decarboxylation (k3D) was 0.17 min-1 in striatum. The elimination of 6-[18F]fluorodopamine (FDA) from striatum suggested an apparent rate constant for monoamine oxidase activity (k7') of 0.055 min-1. 6-[18F]Fluorohomovanillic acid (FHVA) was formed from 6-[18F]fluoro-L-3,4-dihydroxyphenylacetic acid with a rate constant (k11) of 0.083 min-1, and FHVA was eliminated from striatum (k9) with a rate constant of 0.12 min-1. The steady-state concentration ratios of FDA and its metabolites were shown to be functions of these rate constants.
M3 - Journal article
C2 - 7931323
VL - 63
SP - 1675
EP - 1682
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 5
ER -