A "new" supertypic HLA-DP related determinant detected by primed lymphocyte typing (PLT).

Niels Ødum; B Hofmann; J J Hyldig-Nielsen; B K Jakobsen; N Morling; P Platz; Lars P. Ryder; Arne Svejgaard

A "new" supertypic HLA-DP related determinant detected by primed lymphocyte typing (PLT).

Niels Ødum, B Hofmann, J J Hyldig-Nielsen, B K Jakobsen, N Morling, P Platz, Lars P. Ryder, Arne Svejgaard

Cellebiologi og Fysiologi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

10 Citationer (Scopus)

Abstract

Udgivelsesdato: 1987-Feb

Originalsprog	Engelsk
Tidsskrift	HLA
Vol/bind	29
Udgave nummer	2
Sider (fra-til)	101-9
Antal sider	8
ISSN	2059-2302
Status	Udgivet - 1987

Bibliografisk note

Keywords: Antibodies, Monoclonal; Epitopes; HLA-D Antigens; HLA-DP Antigens; HLA-DR Antigens; Humans; Lymphocytes

Adgang til dokumentet

http://www.ncbi.nlm.nih.gov/pubmed/2440144

Citationsformater

@article{5b4889c0fda311ddb219000ea68e967b,

title = "A {"}new{"} supertypic HLA-DP related determinant detected by primed lymphocyte typing (PLT).",

abstract = "Primed Lymphocyte Typing (PLT) with local (CDP) and the 9th International Histocompatibility Workshop reagents (GNN) revealed {"}cross-reactions{"} between HLA-DPw6 and the GNN2B but not other DPw2 PLT-cells (GNN2A, CDP2A, CDP2B). We raised and bulk-expanded a well discriminating PLT-reagent (the JET-reagent) from an HLA-DR identical, DP, GNN2A, CDP2A, CDP2B compatible and GNN2B incompatible responder/stimulator combination. The JET-reagent defined a {"}new{"} determinant, JET, which was present in 11% of Danes. The JET determinant was associated with DPw2, w6, and DP-blank: 66% of DPw6 and DP-blank and 20% of DPw2-positive individuals were JET-positive. All JET-positive individuals belonged to this group, and six of these had two DP-antigens. JET segregated with DP-blank in three and with DPw6 in two informative families. In an HLA-DR/DPw6 recombinant family, JET segregated with DPw6 in the recombinant haplotype. The JET PLT-responses against all of six different JET-positive stimulators including the specific stimulator were strongly inhibited by the monoclonal antibody (MoAb) T{\"u}39, which preferentially reacts with DP-molecules, but not by other HLA-specific MoAbs. The results indicate that JET is either a {"}new{"} supertypic DP-related specificity or a determinant, which shares epitopes with DP, of a {"}new{"} locus and in linkage disequilibrium with DP.",

author = "Niels {\O}dum and B Hofmann and Hyldig-Nielsen, {J J} and Jakobsen, {B K} and N Morling and P Platz and Ryder, {Lars P.} and Arne Svejgaard",

note = "Keywords: Antibodies, Monoclonal; Epitopes; HLA-D Antigens; HLA-DP Antigens; HLA-DR Antigens; Humans; Lymphocytes",

year = "1987",

language = "English",

volume = "29",

pages = "101--9",

journal = "HLA",

issn = "2059-2302",

publisher = "Wiley",

number = "2",

}

TY - JOUR

T1 - A "new" supertypic HLA-DP related determinant detected by primed lymphocyte typing (PLT).

AU - Ødum, Niels

AU - Hofmann, B

AU - Hyldig-Nielsen, J J

AU - Jakobsen, B K

AU - Morling, N

AU - Platz, P

AU - Ryder, Lars P.

AU - Svejgaard, Arne

N1 - Keywords: Antibodies, Monoclonal; Epitopes; HLA-D Antigens; HLA-DP Antigens; HLA-DR Antigens; Humans; Lymphocytes

PY - 1987

Y1 - 1987

N2 - Primed Lymphocyte Typing (PLT) with local (CDP) and the 9th International Histocompatibility Workshop reagents (GNN) revealed "cross-reactions" between HLA-DPw6 and the GNN2B but not other DPw2 PLT-cells (GNN2A, CDP2A, CDP2B). We raised and bulk-expanded a well discriminating PLT-reagent (the JET-reagent) from an HLA-DR identical, DP, GNN2A, CDP2A, CDP2B compatible and GNN2B incompatible responder/stimulator combination. The JET-reagent defined a "new" determinant, JET, which was present in 11% of Danes. The JET determinant was associated with DPw2, w6, and DP-blank: 66% of DPw6 and DP-blank and 20% of DPw2-positive individuals were JET-positive. All JET-positive individuals belonged to this group, and six of these had two DP-antigens. JET segregated with DP-blank in three and with DPw6 in two informative families. In an HLA-DR/DPw6 recombinant family, JET segregated with DPw6 in the recombinant haplotype. The JET PLT-responses against all of six different JET-positive stimulators including the specific stimulator were strongly inhibited by the monoclonal antibody (MoAb) Tü39, which preferentially reacts with DP-molecules, but not by other HLA-specific MoAbs. The results indicate that JET is either a "new" supertypic DP-related specificity or a determinant, which shares epitopes with DP, of a "new" locus and in linkage disequilibrium with DP.

AB - Primed Lymphocyte Typing (PLT) with local (CDP) and the 9th International Histocompatibility Workshop reagents (GNN) revealed "cross-reactions" between HLA-DPw6 and the GNN2B but not other DPw2 PLT-cells (GNN2A, CDP2A, CDP2B). We raised and bulk-expanded a well discriminating PLT-reagent (the JET-reagent) from an HLA-DR identical, DP, GNN2A, CDP2A, CDP2B compatible and GNN2B incompatible responder/stimulator combination. The JET-reagent defined a "new" determinant, JET, which was present in 11% of Danes. The JET determinant was associated with DPw2, w6, and DP-blank: 66% of DPw6 and DP-blank and 20% of DPw2-positive individuals were JET-positive. All JET-positive individuals belonged to this group, and six of these had two DP-antigens. JET segregated with DP-blank in three and with DPw6 in two informative families. In an HLA-DR/DPw6 recombinant family, JET segregated with DPw6 in the recombinant haplotype. The JET PLT-responses against all of six different JET-positive stimulators including the specific stimulator were strongly inhibited by the monoclonal antibody (MoAb) Tü39, which preferentially reacts with DP-molecules, but not by other HLA-specific MoAbs. The results indicate that JET is either a "new" supertypic DP-related specificity or a determinant, which shares epitopes with DP, of a "new" locus and in linkage disequilibrium with DP.

M3 - Journal article

C2 - 2440144

SN - 2059-2302

VL - 29

SP - 101

EP - 109

JO - HLA

JF - HLA

IS - 2

ER -