TY - JOUR
T1 - A- or C-chromosomes, does it matter for the transfer of transgenes from Brassica napus
AU - Tomiuk, J.
AU - Hauser, Thure Pavlo
AU - Jørgensen, R.B.
N1 - Key words Safe integration - Chromosome variation - Oilseed rape - Gene transfer - Risk assessment, Brassica rapa
PY - 2000
Y1 - 2000
N2 - Introgression of genes from allotetraploid Brassica napus into its diploid wild relative B. rapa is generally considered to be inevitable. As a means to minimize a potential ecological risk in environments where B. rapa is growing, the insertion of transgenes into chromosome regions of B. napus with a very low probability of transfer to backcross generations with B. rapa has been proposed. Recently, the progeny of four backcross generations between transgenic herbicide-tolerant B. napus and B. rapa was studied in selection experiments (Metz et al. 1997). The rapid decrease in the frequency of herbicide-tolerant plants was explained by selection against the C-chromosomes of B. napus in favor of the homeologous A-chromosomes. Obviously, such C-chromosomes could be potential candidates as safe integration sites for transgenes. We considered these safety aspects using a simple population genetic model. Theory and experiments, however, do not favor the chromosomes of B. napus as safe candidates with respect to the introgression of transgenes into wild populations of B. rapa.
AB - Introgression of genes from allotetraploid Brassica napus into its diploid wild relative B. rapa is generally considered to be inevitable. As a means to minimize a potential ecological risk in environments where B. rapa is growing, the insertion of transgenes into chromosome regions of B. napus with a very low probability of transfer to backcross generations with B. rapa has been proposed. Recently, the progeny of four backcross generations between transgenic herbicide-tolerant B. napus and B. rapa was studied in selection experiments (Metz et al. 1997). The rapid decrease in the frequency of herbicide-tolerant plants was explained by selection against the C-chromosomes of B. napus in favor of the homeologous A-chromosomes. Obviously, such C-chromosomes could be potential candidates as safe integration sites for transgenes. We considered these safety aspects using a simple population genetic model. Theory and experiments, however, do not favor the chromosomes of B. napus as safe candidates with respect to the introgression of transgenes into wild populations of B. rapa.
U2 - 10.1007/s001220051348
DO - 10.1007/s001220051348
M3 - Journal article
SN - 0040-5752
VL - 100
SP - 750
EP - 754
JO - Theoretical and Applied Genetics
JF - Theoretical and Applied Genetics
IS - 5
ER -