Abstract
A rapid and simple assay has been developed which allows specific detection of Listeria monocytogenes within 3.5 h in cultures prepared from suspect food samples and propagated 48 h in selective medium. The assay is based on PCR technology, and uses a specific primer set derived from sequences located down-stream of the hlyA gene. The specificity of the primer set was confirmed by testing 115 L. monocytogenes, 14 L. innocua, 5 L. seeligeri and 4 L. ivanovii isolates. The assay was compared to standard microbiological tests and gave identical results for 83 food samples, including 32 positives. These field trials indicate that the assay developed provides an alternative detection system for L. monocytogenes in foods, which can be used by the food industry.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | International Journal of Food Microbiology |
| Vol/bind | 14 |
| Udgave nummer | 2 |
| Sider (fra-til) | 145-151 |
| Antal sider | 7 |
| ISSN | 0168-1605 |
| DOI | |
| Status | Udgivet - nov. 1991 |
Finansiering
The authors wish to thank Pernille Norskov and Torben Kallesoe for skilled technical assistance; Steins Laboratorium, Ladelund, for providing food samples and performing the reference test, and Dr. W. Fredriksen, Statens Seruminstitut, for supplying human isolates of L. monocytogenes. Dr. D.B. Coilinge is thanked for critically reading the manuscript. This project was funded in part by The Food Research Program of the Danish Ministry of Agriculture, and by the Danish Biotechnology Program TR-880178.0.
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