A sensitive and reproducible method for the determination of subnanogram quantities of immunoglobulin E (IgE)

L K Poulsen; H J Malling; I Søndergaard; B Weeke

A sensitive and reproducible method for the determination of subnanogram quantities of immunoglobulin E (IgE)

L K Poulsen, H J Malling, I Søndergaard, B Weeke

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

14 Citationer (Scopus)

Abstract

An immunoradiometric assay for immunoglobulin E (IgE) using high adsorption polystyrene test tubes as the solid phase (the 'Maxisorp' assay) is described. The sensitivity of the method was found to be 50 pg IgE/ml and the within-assay reproducibility was 3-7%. The accuracy was estimated by means of a comparison between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude that the Maxisorp assay is a fast and reliable assay for IgE determination in cord blood, cell culture supernatants and other highly diluted IgE preparations.

Originalsprog	Engelsk
Tidsskrift	Journal of Immunological Methods
Vol/bind	92
Udgave nummer	1
Sider (fra-til)	131-6
Antal sider	6
ISSN	0022-1759
Status	Udgivet - 21 aug. 1986

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title = "A sensitive and reproducible method for the determination of subnanogram quantities of immunoglobulin E (IgE)",

abstract = "An immunoradiometric assay for immunoglobulin E (IgE) using high adsorption polystyrene test tubes as the solid phase (the 'Maxisorp' assay) is described. The sensitivity of the method was found to be 50 pg IgE/ml and the within-assay reproducibility was 3-7%. The accuracy was estimated by means of a comparison between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude that the Maxisorp assay is a fast and reliable assay for IgE determination in cord blood, cell culture supernatants and other highly diluted IgE preparations.",

keywords = "Antibody Specificity, Binding, Competitive, Cross Reactions, Dose-Response Relationship, Immunologic, Humans, Immunoglobulin A, Immunoglobulin E, Immunoglobulin G, Immunoglobulin M, Immunosorbent Techniques, Radioimmunoassay, Journal Article, Research Support, Non-U.S. Gov't",

author = "Poulsen, {L K} and Malling, {H J} and I S{\o}ndergaard and B Weeke",

year = "1986",

month = aug,

day = "21",

language = "English",

volume = "92",

pages = "131--6",

journal = "Journal of Immunological Methods",

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TY - JOUR

T1 - A sensitive and reproducible method for the determination of subnanogram quantities of immunoglobulin E (IgE)

AU - Poulsen, L K

AU - Malling, H J

AU - Søndergaard, I

AU - Weeke, B

PY - 1986/8/21

Y1 - 1986/8/21

N2 - An immunoradiometric assay for immunoglobulin E (IgE) using high adsorption polystyrene test tubes as the solid phase (the 'Maxisorp' assay) is described. The sensitivity of the method was found to be 50 pg IgE/ml and the within-assay reproducibility was 3-7%. The accuracy was estimated by means of a comparison between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude that the Maxisorp assay is a fast and reliable assay for IgE determination in cord blood, cell culture supernatants and other highly diluted IgE preparations.

AB - An immunoradiometric assay for immunoglobulin E (IgE) using high adsorption polystyrene test tubes as the solid phase (the 'Maxisorp' assay) is described. The sensitivity of the method was found to be 50 pg IgE/ml and the within-assay reproducibility was 3-7%. The accuracy was estimated by means of a comparison between the Maxisorp assay and paper radioimmunosorbent test (PRIST) and a correlation coefficient of 0.98 (P less than 0.001) was obtained. We conclude that the Maxisorp assay is a fast and reliable assay for IgE determination in cord blood, cell culture supernatants and other highly diluted IgE preparations.

KW - Antibody Specificity

KW - Binding, Competitive

KW - Cross Reactions

KW - Dose-Response Relationship, Immunologic

KW - Humans

KW - Immunoglobulin A

KW - Immunoglobulin E

KW - Immunoglobulin G

KW - Immunoglobulin M

KW - Immunosorbent Techniques

KW - Radioimmunoassay

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - Journal article

C2 - 3528297

SN - 0022-1759

VL - 92

SP - 131

EP - 136

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1

ER -