Aberrant brain microRNA target and miRISC gene expression in the anx/anx anorexia mouse model

Josep M Mercader; Juan R González; Juan José Lozano; Mads Bak; Sakari Kauppinen; Lauro Sumoy; Mara Dierssen; Fernando Fernández-Aranda; Joana Visa; Mònica Gratacòs; Xavier Estivill

doi:10.1016/j.gene.2012.01.057

Aberrant brain microRNA target and miRISC gene expression in the anx/anx anorexia mouse model

Josep M Mercader, Juan R González, Juan José Lozano, Mads Bak, Sakari Kauppinen, Lauro Sumoy, Mara Dierssen, Fernando Fernández-Aranda, Joana Visa, Mònica Gratacòs, Xavier Estivill

Institut for Cellulær og Molekylær Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

14 Citationer (Scopus)

Abstract

The anorexia mouse model, anx/anx, carries a spontaneous mutation not yet identified and homozygous mutants are characterized by anorexia-cachexia, hyperactivity, and ataxia. In order to test if the microRNA function was altered in these mice, hypothalamus and cortex transcriptomes were evaluated and the data was analyzed taking into account the presence of microRNA target sites. Subsequent validation of the expression of a subset of miRISC coding genes and microRNA targets was performed by TaqMan real time PCR. In anx/anx hypothalamus we found that predicted microRNA targets were preferentially upregulated in a linearly dependent manner according to the number of microRNA target sites in each mRNA (p=10(-139)). Conversely, we observed that in anx/anx cortex mRNAs predicted to be targeted by microRNAs were preferentially downregulated (p

Originalsprog	Engelsk
Tidsskrift	Gene
Vol/bind	497
Udgave nummer	2
Sider (fra-til)	181-90
Antal sider	10
ISSN	0378-1119
DOI	https://doi.org/10.1016/j.gene.2012.01.057
Status	Udgivet - 15 apr. 2012

Adgang til dokumentet

10.1016/j.gene.2012.01.057

Citationsformater

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title = "Aberrant brain microRNA target and miRISC gene expression in the anx/anx anorexia mouse model",

abstract = "The anorexia mouse model, anx/anx, carries a spontaneous mutation not yet identified and homozygous mutants are characterized by anorexia-cachexia, hyperactivity, and ataxia. In order to test if the microRNA function was altered in these mice, hypothalamus and cortex transcriptomes were evaluated and the data was analyzed taking into account the presence of microRNA target sites. Subsequent validation of the expression of a subset of miRISC coding genes and microRNA targets was performed by TaqMan real time PCR. In anx/anx hypothalamus we found that predicted microRNA targets were preferentially upregulated in a linearly dependent manner according to the number of microRNA target sites in each mRNA (p=10(-139)). Conversely, we observed that in anx/anx cortex mRNAs predicted to be targeted by microRNAs were preferentially downregulated (p",

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T1 - Aberrant brain microRNA target and miRISC gene expression in the anx/anx anorexia mouse model

AU - Mercader, Josep M

AU - González, Juan R

AU - Lozano, Juan José

AU - Bak, Mads

AU - Kauppinen, Sakari

AU - Sumoy, Lauro

AU - Dierssen, Mara

AU - Fernández-Aranda, Fernando

AU - Visa, Joana

AU - Gratacòs, Mònica

AU - Estivill, Xavier

PY - 2012/4/15

Y1 - 2012/4/15

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U2 - 10.1016/j.gene.2012.01.057

DO - 10.1016/j.gene.2012.01.057

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