Acute effects of vascular modifying agents in solid tumors assessed by noninvasive laser Doppler flowmetry and near infrared spectroscopy.

Michael Kragh; Bjørn Quistorff; Michael R Horsman; Paul E G Kristjansen

doi:10.1038/sj/neo/7900230

Acute effects of vascular modifying agents in solid tumors assessed by noninvasive laser Doppler flowmetry and near infrared spectroscopy.

Michael Kragh, Bjørn Quistorff, Michael R Horsman, Paul E G Kristjansen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

18 Citationer (Scopus)

Abstract

Udgivelsesdato: null-null

Originalsprog	Engelsk
Tidsskrift	NeoPlasia
Vol/bind	4
Udgave nummer	3
Sider (fra-til)	263-7
Antal sider	4
ISSN	1522-8002
DOI	https://doi.org/10.1038/sj/neo/7900230
Status	Udgivet - 2002

Bibliografisk note

Keywords: Animals; Antineoplastic Agents; Antineoplastic Agents, Phytogenic; Female; Flavonoids; Hydralazine; Laser-Doppler Flowmetry; Mice; Mice, Inbred C3H; Neoplasm Transplantation; Neoplasms; Neovascularization, Pathologic; Niacinamide; Spectroscopy, Near-Infrared; Stilbenes; Time Factors; Tumor Cells, Cultured; Vasodilator Agents; Xanthenes; Xanthones

Adgang til dokumentet

10.1038/sj/neo/7900230

Citationsformater

@article{ecdf3e10abfa11ddb5e9000ea68e967b,

title = "Acute effects of vascular modifying agents in solid tumors assessed by noninvasive laser Doppler flowmetry and near infrared spectroscopy.",

abstract = "The potential of noninvasive laser Doppler flowmetry (LDF) and near infrared spectroscopy (NIRS) to detect acute effects of different vascular-modifying agents on perfusion and blood volume in tumors was evaluated. C3H mouse mammary carcinomas (approximately 200 mm(3)) in the rear foot of CDF1 mice were treated with flavone acetic acid (FAA, 150 mg/kg), 5,6-dimethylxanthenone-4-acetic acid (DMXAA, 20 mg/kg), combretastatin A-4 disodium phosphate (CA4DP, 250 mg/kg), hydralazine (HDZ, 5 mg/kg), or nicotinamide (NTA, 500 mg/kg). Tumor perfusion before and after treatment was evaluated by noninvasive LDF, using a 41 degrees C heated custom-built LDF probe with four integrated laser/receiver units, and tumor blood volume was estimated by NIRS, using light guide coupled reflectance measurements at 800+/-10 nm. FAA, DMXAA, CA4DP, and HDZ significantly decreased tumor perfusion by 50%, 47%, 73%, and 78%, respectively. In addition, FAA, DMXAA, and HDZ significantly reduced the blood volume within the tumor, indicating that these compounds to some degree shunted blood from the tumor to adjacent tissue, HDZ being most potent. CA4DP caused no change in the tumor blood volume, indicating that the mechanism of action of CA4DP was vascular shut down with the blood pool trapped in the tumor. NTA caused no change in either tumor perfusion or tumor blood volume. We conclude that noninvasive LDF and NIRS can determine acute effects of vascular modifying agents on tumor perfusion and blood volume.",

author = "Michael Kragh and Bj{\o}rn Quistorff and Horsman, {Michael R} and Kristjansen, {Paul E G}",

note = "Keywords: Animals; Antineoplastic Agents; Antineoplastic Agents, Phytogenic; Female; Flavonoids; Hydralazine; Laser-Doppler Flowmetry; Mice; Mice, Inbred C3H; Neoplasm Transplantation; Neoplasms; Neovascularization, Pathologic; Niacinamide; Spectroscopy, Near-Infrared; Stilbenes; Time Factors; Tumor Cells, Cultured; Vasodilator Agents; Xanthenes; Xanthones",

year = "2002",

doi = "10.1038/sj/neo/7900230",

language = "English",

volume = "4",

pages = "263--7",

journal = "Neoplasia",

issn = "1522-8002",

publisher = "Neoplasia Press",

number = "3",

}

TY - JOUR

T1 - Acute effects of vascular modifying agents in solid tumors assessed by noninvasive laser Doppler flowmetry and near infrared spectroscopy.

AU - Kragh, Michael

AU - Quistorff, Bjørn

AU - Horsman, Michael R

AU - Kristjansen, Paul E G

N1 - Keywords: Animals; Antineoplastic Agents; Antineoplastic Agents, Phytogenic; Female; Flavonoids; Hydralazine; Laser-Doppler Flowmetry; Mice; Mice, Inbred C3H; Neoplasm Transplantation; Neoplasms; Neovascularization, Pathologic; Niacinamide; Spectroscopy, Near-Infrared; Stilbenes; Time Factors; Tumor Cells, Cultured; Vasodilator Agents; Xanthenes; Xanthones

PY - 2002

Y1 - 2002

N2 - The potential of noninvasive laser Doppler flowmetry (LDF) and near infrared spectroscopy (NIRS) to detect acute effects of different vascular-modifying agents on perfusion and blood volume in tumors was evaluated. C3H mouse mammary carcinomas (approximately 200 mm(3)) in the rear foot of CDF1 mice were treated with flavone acetic acid (FAA, 150 mg/kg), 5,6-dimethylxanthenone-4-acetic acid (DMXAA, 20 mg/kg), combretastatin A-4 disodium phosphate (CA4DP, 250 mg/kg), hydralazine (HDZ, 5 mg/kg), or nicotinamide (NTA, 500 mg/kg). Tumor perfusion before and after treatment was evaluated by noninvasive LDF, using a 41 degrees C heated custom-built LDF probe with four integrated laser/receiver units, and tumor blood volume was estimated by NIRS, using light guide coupled reflectance measurements at 800+/-10 nm. FAA, DMXAA, CA4DP, and HDZ significantly decreased tumor perfusion by 50%, 47%, 73%, and 78%, respectively. In addition, FAA, DMXAA, and HDZ significantly reduced the blood volume within the tumor, indicating that these compounds to some degree shunted blood from the tumor to adjacent tissue, HDZ being most potent. CA4DP caused no change in the tumor blood volume, indicating that the mechanism of action of CA4DP was vascular shut down with the blood pool trapped in the tumor. NTA caused no change in either tumor perfusion or tumor blood volume. We conclude that noninvasive LDF and NIRS can determine acute effects of vascular modifying agents on tumor perfusion and blood volume.

AB - The potential of noninvasive laser Doppler flowmetry (LDF) and near infrared spectroscopy (NIRS) to detect acute effects of different vascular-modifying agents on perfusion and blood volume in tumors was evaluated. C3H mouse mammary carcinomas (approximately 200 mm(3)) in the rear foot of CDF1 mice were treated with flavone acetic acid (FAA, 150 mg/kg), 5,6-dimethylxanthenone-4-acetic acid (DMXAA, 20 mg/kg), combretastatin A-4 disodium phosphate (CA4DP, 250 mg/kg), hydralazine (HDZ, 5 mg/kg), or nicotinamide (NTA, 500 mg/kg). Tumor perfusion before and after treatment was evaluated by noninvasive LDF, using a 41 degrees C heated custom-built LDF probe with four integrated laser/receiver units, and tumor blood volume was estimated by NIRS, using light guide coupled reflectance measurements at 800+/-10 nm. FAA, DMXAA, CA4DP, and HDZ significantly decreased tumor perfusion by 50%, 47%, 73%, and 78%, respectively. In addition, FAA, DMXAA, and HDZ significantly reduced the blood volume within the tumor, indicating that these compounds to some degree shunted blood from the tumor to adjacent tissue, HDZ being most potent. CA4DP caused no change in the tumor blood volume, indicating that the mechanism of action of CA4DP was vascular shut down with the blood pool trapped in the tumor. NTA caused no change in either tumor perfusion or tumor blood volume. We conclude that noninvasive LDF and NIRS can determine acute effects of vascular modifying agents on tumor perfusion and blood volume.

U2 - 10.1038/sj/neo/7900230

DO - 10.1038/sj/neo/7900230

M3 - Journal article

C2 - 11988846

VL - 4

SP - 263

EP - 267

JO - Neoplasia

JF - Neoplasia

SN - 1522-8002

IS - 3

ER -