Acyl-coenzyme A binding protein (ACBP)

Birthe B. Kragelund, J. Knudsen, Flemming M. Poulsen

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

103 Citationer (Scopus)

Abstract

Acyl-coenzyme A binding proteins are known from a large group of eukaryote species and to bind a long chain length acyl-CoA ester with very high affinity. Detailed biochemical mapping of ligand binding properties has been obtained as well as in-depth structural studies on the bovine apo-protein and of the complex with palmitoyl-CoA using NMR spectroscopy. In the four alpha-helix bundle structure, a set of 21 highly conserved residues present in more that 90% of all known sequences of acyl-coenzyme A binding proteins constitutes three separate mini-cores. These residues are predominantly located at the helix-helix interfaces. From studies of a large set of mutant proteins the role of the conserved residues has been related to structure, function, folding and stability.

OriginalsprogEngelsk
TidsskriftBiochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
Vol/bind1441
Udgave nummer2-3
Sider (fra-til)150-161
Antal sider12
ISSN1388-1981
DOI
StatusUdgivet - 1999
Udgivet eksterntJa

Emneord

  • Amino Acid Sequence
  • Animals
  • Carrier Proteins
  • Diazepam Binding Inhibitor
  • Humans
  • Models, Molecular
  • Molecular Sequence Data
  • Molecular Structure
  • Mutagenesis
  • Sequence Alignment

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