Adaptation of intestinal hydrolases to starvation in rats: effect of thyroid function

M Galluser; R Belkhou; J N Freund; I Duluc; N Torp; Erik Michael Danielsen; F Raul

Adaptation of intestinal hydrolases to starvation in rats: effect of thyroid function

M Galluser, R Belkhou, J N Freund, I Duluc, N Torp, Erik Michael Danielsen, F Raul

Institut for Cellulær og Molekylær Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

14 Citationer (Scopus)

Abstract

Udgivelsesdato: 1991-null

Originalsprog	Engelsk
Tidsskrift	Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
Vol/bind	161
Udgave nummer	4
Sider (fra-til)	357-61
Antal sider	4
ISSN	0174-1578
Status	Udgivet - 1991

Bibliografisk note

Keywords: Adaptation, Physiological; Aminopeptidases; Animals; Hydrolases; Intestine, Small; Lactase; Male; Proteins; RNA, Messenger; Rats; Rats, Inbred Strains; Starvation; Sucrase; Thyroid Gland; Thyroidectomy; beta-Galactosidase

Citationsformater

@article{ca8f7e806c8711de8bc9000ea68e967b,

title = "Adaptation of intestinal hydrolases to starvation in rats: effect of thyroid function",

abstract = "The effects of long-term starvation on the activities of sucrase, lactase, and aminopeptidase, and on their respective mRNA were determined in the small intestine of thyroidectomized and sham-operated adult rats. Thyroidectomy reduced the protein loss at the level of the intestinal brush border membranes during starvation. Prolonged fasting caused a significant decrease in sucrase activity, but thyroidectomy partly prevented this effect. However, the amount of the corresponding mRNA dropped during long term starvation without incidence of thyroidectomy. Lactase activity in the brush border membranes was increased by starvation, and thyroidectomy caused a further elevation of the enzyme activity. Simultaneously, lactase mRNA content rose only slightly compared to the enzyme activity. Aminopeptidase activity and mRNA content decreased during starvation and thyroidectomy did not prevent this process. These results indicate that intestinal hydrolases respond non-coordinately to long-term food deprivation. In addition, the thyroid status of the animals has a direct influence on the adaptation of several brush border hydrolases to starvation. This suggests that the drop in plasma thyroid hormones during fasting allows a better maintenance of protein content and of hydrolase activities in the brush border membranes of the small intestine. These adaptive processes seemed to be partly controlled at a post-transcriptional level.",

author = "M Galluser and R Belkhou and Freund, {J N} and I Duluc and N Torp and Danielsen, {Erik Michael} and F Raul",

note = "Keywords: Adaptation, Physiological; Aminopeptidases; Animals; Hydrolases; Intestine, Small; Lactase; Male; Proteins; RNA, Messenger; Rats; Rats, Inbred Strains; Starvation; Sucrase; Thyroid Gland; Thyroidectomy; beta-Galactosidase",

year = "1991",

language = "English",

volume = "161",

pages = "357--61",

journal = "Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology",

issn = "0174-1578",

publisher = "Springer",

number = "4",

}

TY - JOUR

T1 - Adaptation of intestinal hydrolases to starvation in rats: effect of thyroid function

AU - Galluser, M

AU - Belkhou, R

AU - Freund, J N

AU - Duluc, I

AU - Torp, N

AU - Danielsen, Erik Michael

AU - Raul, F

N1 - Keywords: Adaptation, Physiological; Aminopeptidases; Animals; Hydrolases; Intestine, Small; Lactase; Male; Proteins; RNA, Messenger; Rats; Rats, Inbred Strains; Starvation; Sucrase; Thyroid Gland; Thyroidectomy; beta-Galactosidase

PY - 1991

Y1 - 1991

N2 - The effects of long-term starvation on the activities of sucrase, lactase, and aminopeptidase, and on their respective mRNA were determined in the small intestine of thyroidectomized and sham-operated adult rats. Thyroidectomy reduced the protein loss at the level of the intestinal brush border membranes during starvation. Prolonged fasting caused a significant decrease in sucrase activity, but thyroidectomy partly prevented this effect. However, the amount of the corresponding mRNA dropped during long term starvation without incidence of thyroidectomy. Lactase activity in the brush border membranes was increased by starvation, and thyroidectomy caused a further elevation of the enzyme activity. Simultaneously, lactase mRNA content rose only slightly compared to the enzyme activity. Aminopeptidase activity and mRNA content decreased during starvation and thyroidectomy did not prevent this process. These results indicate that intestinal hydrolases respond non-coordinately to long-term food deprivation. In addition, the thyroid status of the animals has a direct influence on the adaptation of several brush border hydrolases to starvation. This suggests that the drop in plasma thyroid hormones during fasting allows a better maintenance of protein content and of hydrolase activities in the brush border membranes of the small intestine. These adaptive processes seemed to be partly controlled at a post-transcriptional level.

AB - The effects of long-term starvation on the activities of sucrase, lactase, and aminopeptidase, and on their respective mRNA were determined in the small intestine of thyroidectomized and sham-operated adult rats. Thyroidectomy reduced the protein loss at the level of the intestinal brush border membranes during starvation. Prolonged fasting caused a significant decrease in sucrase activity, but thyroidectomy partly prevented this effect. However, the amount of the corresponding mRNA dropped during long term starvation without incidence of thyroidectomy. Lactase activity in the brush border membranes was increased by starvation, and thyroidectomy caused a further elevation of the enzyme activity. Simultaneously, lactase mRNA content rose only slightly compared to the enzyme activity. Aminopeptidase activity and mRNA content decreased during starvation and thyroidectomy did not prevent this process. These results indicate that intestinal hydrolases respond non-coordinately to long-term food deprivation. In addition, the thyroid status of the animals has a direct influence on the adaptation of several brush border hydrolases to starvation. This suggests that the drop in plasma thyroid hormones during fasting allows a better maintenance of protein content and of hydrolase activities in the brush border membranes of the small intestine. These adaptive processes seemed to be partly controlled at a post-transcriptional level.

M3 - Journal article

C2 - 1939743

SN - 0174-1578

VL - 161

SP - 357

EP - 361

JO - Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology

JF - Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology

IS - 4

ER -