Abstract
Originalsprog | Engelsk |
---|---|
Tidsskrift | Plant Physiology |
Vol/bind | 126 |
Udgave nummer | 1 |
Sider (fra-til) | 210-221 |
ISSN | 0032-0889 |
Status | Udgivet - 2001 |
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Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1¿5)-a -L-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato1. / Orfila, C.; Seymour, G.B; Willats, William George Tycho; Huxham, I.M.; Jarvis, M.C.; Dover, C.J.; Thompson, A.J.; Knox, J.P.
I: Plant Physiology, Bind 126, Nr. 1, 2001, s. 210-221.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Altered Middle Lamella Homogalacturonan and Disrupted Deposition of (1¿5)-a -L-Arabinan in the Pericarp of Cnr, a Ripening Mutant of Tomato1
AU - Orfila, C.
AU - Seymour, G.B
AU - Willats, William George Tycho
AU - Huxham, I.M.
AU - Jarvis, M.C.
AU - Dover, C.J.
AU - Thompson, A.J.
AU - Knox, J.P.
PY - 2001
Y1 - 2001
N2 - Cnr (colorless non-ripening) is a pleiotropic tomato (Lycopersicon esculentum) fruit ripening mutant with altered tissue properties including weaker cell-to-cell contacts in the pericarp (A.J. Thompson, M. Tor, C.S. Barry, J. Vrebalov, C. Orfila, M.C. Jarvis, J.J. Giovannoni, D. Grierson, G.B. Seymour [1999] Plant Physiol 120: 383-390). Whereas the genetic basis of the Cnr mutation is being identified by molecular analyses, here we report the identification of cell biological factors underlying the Cnr texture phenotype. In comparison with wild type, ripe-stage Cnr fruits have stronger, non-swollen cell walls (CW) throughout the pericarp and extensive intercellular space in the inner pericarp. Using electron energy loss spectroscopy imaging of calcium-binding capacity and anti-homogalacturonan (HG) antibody probes (PAM1 and JIM5) we demonstrate that maturation processes involving middle lamella HG are altered in Cnr fruit, resulting in the absence or a low level of HG-/calcium-based cell adhesion. We also demonstrate that the deposition of (15)--L-arabinan is disrupted in Cnr pericarp CW and that this disruption occurs prior to fruit ripening. The relationship between the disruption of (15)--L-arabinan deposition in pericarp CW and the Cnr phenotype is discussed.
AB - Cnr (colorless non-ripening) is a pleiotropic tomato (Lycopersicon esculentum) fruit ripening mutant with altered tissue properties including weaker cell-to-cell contacts in the pericarp (A.J. Thompson, M. Tor, C.S. Barry, J. Vrebalov, C. Orfila, M.C. Jarvis, J.J. Giovannoni, D. Grierson, G.B. Seymour [1999] Plant Physiol 120: 383-390). Whereas the genetic basis of the Cnr mutation is being identified by molecular analyses, here we report the identification of cell biological factors underlying the Cnr texture phenotype. In comparison with wild type, ripe-stage Cnr fruits have stronger, non-swollen cell walls (CW) throughout the pericarp and extensive intercellular space in the inner pericarp. Using electron energy loss spectroscopy imaging of calcium-binding capacity and anti-homogalacturonan (HG) antibody probes (PAM1 and JIM5) we demonstrate that maturation processes involving middle lamella HG are altered in Cnr fruit, resulting in the absence or a low level of HG-/calcium-based cell adhesion. We also demonstrate that the deposition of (15)--L-arabinan is disrupted in Cnr pericarp CW and that this disruption occurs prior to fruit ripening. The relationship between the disruption of (15)--L-arabinan deposition in pericarp CW and the Cnr phenotype is discussed.
M3 - Journal article
VL - 126
SP - 210
EP - 221
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 1
ER -