AMPKα2 is a skeletal muscle stem cell intrinsic regulator of myonuclear accretion

Anita Kneppers, Sabrina Ben Larbi, Marine Theret, Audrey Saugues, Carole Dabadie, Linda Gsaier, Arnaud Ferry, Philipp Rhein, Julien Gondin, Kei Sakamoto, Rémi Mounier*

*Corresponding author af dette arbejde

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Abstract

Due to the post-mitotic nature of skeletal muscle fibers, adult muscle maintenance relies on dedicated muscle stem cells (MuSCs). In most physiological contexts, MuSCs support myofiber homeostasis by contributing to myonuclear accretion, which requires a coordination of cell-type specific events between the myofiber and MuSCs. Here, we addressed the role of the kinase AMPKα2 in the coordination of these events supporting myonuclear accretion. We demonstrate that AMPKα2 deletion impairs skeletal muscle regeneration. Through in vitro assessments of MuSC myogenic fate and EdU-based cell tracing, we reveal a MuSC-specific role of AMPKα2 in the regulation of myonuclear accretion, which is mediated by phosphorylation of the non-metabolic substrate BAIAP2. Similar cell tracing in vivo shows that AMPKα2 knockout mice have a lower rate of myonuclear accretion during regeneration, and that MuSC-specific AMPKα2 deletion decreases myonuclear accretion in response to myofiber contraction. Together, this demonstrates that AMPKα2 is a MuSC-intrinsic regulator of myonuclear accretion.

OriginalsprogEngelsk
Artikelnummer108343
TidsskriftiScience
Vol/bind26
Udgave nummer12
Antal sider17
ISSN2589-0042
DOI
StatusUdgivet - 2023

Bibliografisk note

Funding Information:
This work was supported by Centre National de la Recherche Scientifique , the Association Française Contre les Myopathies-Téléthon (Alliance MyoNeurALP1 and MyoNeurALP2) and the Agence Nationale de la Recherche (ANR JCJC # ANR-22-CE14-0032-01 ). A.K. was supported by a Kootstra Talent Fellowship (Maastricht University) and a Marie Skłodowska-Curie Individual Fellowship (#896544). A.S. was supported by Fondation pour la Recherche Médicale (#ECO202206015552). L.G. was supported by Ligue Contre le Cancer (#GB/MA/SC 12627).

Publisher Copyright:
© 2023

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