An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020

Anna S Fomsgaard; Maiken Worsøe Rosenstierne

doi:10.2807/1560-7917.ES.2020.25.14.2000398

An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020

Anna S Fomsgaard, Maiken Worsøe Rosenstierne

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

141 Citationer (Scopus)

34 Downloads (Pure)

Abstract

The World Health Organization has declared COVID-19 caused by the newly discovered SARS-CoV-2 a pandemic. Due to growing demand for reagents and/or kits to extract SARS-CoV-2 RNA for subsequent RT-qPCR diagnostics, there is a worldwide risk of shortages. With a detection sensitivity of 97.4% (95% CI: 86.2-99.9%), we describe a simple, fast, alternative workflow for molecular detection of SARS-CoV-2, where samples are simply heat-processed for 5 min at 98 °C before a commonly-used RT-qPCR procedure.

Originalsprog	Engelsk
Tidsskrift	Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
Vol/bind	25
Udgave nummer	14
Antal sider	6
ISSN	1025-496X
DOI	https://doi.org/10.2807/1560-7917.ES.2020.25.14.2000398
Status	Udgivet - 2020
Udgivet eksternt	Ja

Adgang til dokumentet

10.2807/1560-7917.ES.2020.25.14.2000398Licens: CC BY

An alternative workflow for molecular detection of SARS-CoV-2 – escape from the NA extraction kit-shortage, Copenhagen, Denmark, MarchForlagets udgivne version, 167 KBLicens: CC BY

Citationsformater

An alternative workflow for molecular detection of SARS-CoV-2 - escape from the NA extraction kit-shortage, Copenhagen, Denmark, March 2020. / Fomsgaard, Anna S; Rosenstierne, Maiken Worsøe.
I: Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin, Bind 25, Nr. 14, 2020.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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