TY - JOUR
T1 - An artificial synthetic pathway for acetoin, 2,3-butanediol, and 2-butanol production from ethanol using cell free multi-enzyme catalysis
AU - Zhang, Liaoyuan
AU - Singh, Raushan
AU - Sivakumar, D.
AU - Guo, Zewang
AU - Li, Jiahuan
AU - Chen, Fanbing
AU - He, Yuanzhi
AU - Guan, Xiong
AU - Kang, Yun Chan
AU - Lee, Jung Kul
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.
AB - Upgrading ethanol to higher order alcohols is desired but difficult using current biotechnological methods. In this study, we designed a completely artificial reaction pathway for upgrading ethanol to acetoin, 2,3-butanediol, and 2-butanol in a cell-free bio-system composed of ethanol dehydrogenase, formolase, 2,3-butanediol dehydrogenase, diol dehydratase, and NADH oxidase. Under optimized conditions, acetoin, 2,3-butanediol, and 2-butanol were produced at 88.78%, 88.28%, and 27.25% of the theoretical yield from 100 mM ethanol, respectively. These results demonstrate that this artificial synthetic pathway is an environmentally-friendly novel approach for upgrading bio-ethanol to acetoin, 2,3-butanediol, and 2-butanol.
U2 - 10.1039/c7gc02898a
DO - 10.1039/c7gc02898a
M3 - Journal article
AN - SCOPUS:85040191293
SN - 1463-9262
VL - 20
SP - 230
EP - 242
JO - Green Chemistry
JF - Green Chemistry
IS - 1
ER -