Analysis of activated GAPs and GEFs in cell lysates

Rafael García-Mata, Krister Wennerberg, William T Arthur, Nicole K Noren, Shawn M Ellerbroek, Keith Burridge

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

164 Citationer (Scopus)

Abstract

An assay was developed that allows the precipitation of the active pools of Rho-GEFs, Rho-GAPs, or effectors from cell or tissue lysates. This assay can be used to identify GEFs, GAPs, and effectors involved in specific cellular pathways to determine their GTPase specificity and to monitor the temporal activation of GEFs and GAPs in response to upstream signals.

OriginalsprogEngelsk
BogserieMethods in Enzymology
Vol/bind406
Sider (fra-til)425-37
Antal sider13
ISSN0076-6879
DOI
StatusUdgivet - 2006
Udgivet eksterntJa

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