Analysis of antibody inhibition of PfEMP1 binding by competition ELISA

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningpeer review

Abstract

Plasmodium falciparum express variant antigens on the surface of infected erythrocytes (IEs). These include proteins of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) family. PfEMP1 proteins mediate binding of IEs to various human endothelial receptors and induce antibodies response during natural infections. In this chapter, we describe a competition ELISA that is an assay for antibodies neutralizing binding of PfEMP1 proteins to their cognate receptor.

OriginalsprogEngelsk
TitelMalaria Immunology : Targeting the Surface of Infected Erythrocytes
Antal sider7
Vol/bind2470
ForlagHumana Press
Publikationsdato2022
Sider485-491
ISBN (Elektronisk)978-1-0716-2189-9
DOI
StatusUdgivet - 2022
NavnMethods in molecular biology (Clifton, N.J.)
ISSN1064-3745

Bibliografisk note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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