TY - JOUR
T1 - Analysis of SOX2 expression in developing human testis and germ cell neoplasia
AU - Sonne, Si Brask
AU - Perrett, Rebecca M.
AU - Nielsen, John Erik
AU - Baxter, Melissa A.
AU - Kristensen, David Møbjerg
AU - Leffers, Henrik
AU - Hanley, Neil A.
AU - Rajpert-De-Meyts, Ewa
N1 - Key words: carcinoma in situ (CIS), intratubular germ cell neoplasia (ITGCN), testicular germ cell tumour (TGCT), Sertoli cell, pluripotency
PY - 2010
Y1 - 2010
N2 - The transcriptional regulators of pluripotency, POU5F1 (OCT4), NANOG and SOX2, are highly expressed in embryonal carcinoma (EC). In contrast to OCT4 and NANOG, SOX2 has not been demonstrated in the early human germ cell lineage or carcinoma in situ (CIS), the precursor for testicular germ cell tumours (TGCTs). Here, we have analysed SOX2 expression in CIS and overt TGCTs, as well as normal second and third trimester fetal, prepubertal and adult testes by in situ hybridisation and immunohistochemistry using three different antibodies. In contrast to earlier studies, we detected SOX2 mRNA in most CIS cells. We also detected speckled nuclear SOX2 immunoreactivity in CIS cells with one primary antibody, which was not apparent with other primary antibodies. The results demonstrate SOX2 gene expression in CIS for the first time and raise the possibility of post-transcriptional regulation, most likely sumoylation as a mechanism for limiting SOX2 action in these cells.
AB - The transcriptional regulators of pluripotency, POU5F1 (OCT4), NANOG and SOX2, are highly expressed in embryonal carcinoma (EC). In contrast to OCT4 and NANOG, SOX2 has not been demonstrated in the early human germ cell lineage or carcinoma in situ (CIS), the precursor for testicular germ cell tumours (TGCTs). Here, we have analysed SOX2 expression in CIS and overt TGCTs, as well as normal second and third trimester fetal, prepubertal and adult testes by in situ hybridisation and immunohistochemistry using three different antibodies. In contrast to earlier studies, we detected SOX2 mRNA in most CIS cells. We also detected speckled nuclear SOX2 immunoreactivity in CIS cells with one primary antibody, which was not apparent with other primary antibodies. The results demonstrate SOX2 gene expression in CIS for the first time and raise the possibility of post-transcriptional regulation, most likely sumoylation as a mechanism for limiting SOX2 action in these cells.
U2 - 10.1387/ijdb.082668ss
DO - 10.1387/ijdb.082668ss
M3 - Journal article
C2 - 19876845
SN - 0214-6282
VL - 54
SP - 755
EP - 760
JO - International Journal of Developmental Biology
JF - International Journal of Developmental Biology
IS - 4
ER -