Anaphase onset before complete DNA replication with intact checkpoint responses.

Jordi Torres-Rosell, Giacomo De Piccoli, Violeta Cordon-Preciado, Sarah Farmer, Adam Jarmuz, Felix Machin, Philippe Pasero, Michael Lisby, James E Haber, Luis Aragón

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

113 Citationer (Scopus)

Abstract

Cellular checkpoints prevent mitosis in the presence of stalled replication forks. Whether checkpoints also ensure the completion of DNA replication before mitosis is unknown. Here, we show that in yeast smc5-smc6 mutants, which are related to cohesin and condensin, replication is delayed, most significantly at natural replication-impeding loci like the ribosomal DNA gene cluster. In the absence of Smc5-Smc6, chromosome nondisjunction occurs as a consequence of mitotic entry with unfinished replication despite intact checkpoint responses. Eliminating processes that obstruct replication fork progression restores the temporal uncoupling between replication and segregation in smc5-smc6 mutants. We propose that the completion of replication is not under the surveillance of known checkpoints.
Udgivelsesdato: 2007-Mar-9
OriginalsprogEngelsk
TidsskriftScience
Vol/bind315
Udgave nummer5817
Sider (fra-til)1411-5
Antal sider4
ISSN0036-8075
DOI
StatusUdgivet - 2007

Bibliografisk note

Keywords: Anaphase; Cell Cycle Proteins; Chromosome Segregation; Chromosomes, Fungal; DNA Breaks, Double-Stranded; DNA Damage; DNA Replication; DNA, Fungal; DNA, Ribosomal; Genes, Fungal; Genes, rRNA; Metaphase; Mitosis; Models, Genetic; Mutation; Nondisjunction, Genetic; Protein-Serine-Threonine Kinases; S Phase; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins

Citationsformater