Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain

Christian Ansgar Hundahl; Gregg C Allen; Jens Hannibal; Katrine Kjaer; Jens F Rehfeld; Sylvia Dewilde; Jens R Nyengaard; Jesper Kelsen; Anders Hay-Schmidt

doi:10.1016/j.brainres.2010.03.056

Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain

Christian Ansgar Hundahl, Gregg C Allen, Jens Hannibal, Katrine Kjaer, Jens F Rehfeld, Sylvia Dewilde, Jens R Nyengaard, Jesper Kelsen, Anders Hay-Schmidt

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

92 Citationer (Scopus)

Abstract

Udgivelsesdato: 2010-May-17

Originalsprog	Engelsk
Tidsskrift	Brain Research
Vol/bind	1331
Sider (fra-til)	58-73
Antal sider	16
ISSN	0006-8993
DOI	https://doi.org/10.1016/j.brainres.2010.03.056
Status	Udgivet - 2010

Bibliografisk note

Adgang til dokumentet

10.1016/j.brainres.2010.03.056

Citationsformater

@article{a0255450b74d11df825b000ea68e967b,

title = "Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain",

abstract = "The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb.",

author = "Hundahl, {Christian Ansgar} and Allen, {Gregg C} and Jens Hannibal and Katrine Kjaer and Rehfeld, {Jens F} and Sylvia Dewilde and Nyengaard, {Jens R} and Jesper Kelsen and Anders Hay-Schmidt",

note = "Keywords: Animals; Brain; Globins; Immunohistochemistry; In Situ Hybridization; Male; Mice; Mice, Inbred C57BL; Microscopy, Immunoelectron; Nerve Tissue Proteins; Neurons; Nitric Oxide; RNA, Messenger",

year = "2010",

doi = "10.1016/j.brainres.2010.03.056",

language = "English",

volume = "1331",

pages = "58--73",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier",

}

TY - JOUR

T1 - Anatomical characterization of cytoglobin and neuroglobin mRNA and protein expression in the mouse brain

AU - Hundahl, Christian Ansgar

AU - Allen, Gregg C

AU - Hannibal, Jens

AU - Kjaer, Katrine

AU - Rehfeld, Jens F

AU - Dewilde, Sylvia

AU - Nyengaard, Jens R

AU - Kelsen, Jesper

AU - Hay-Schmidt, Anders

N1 - Keywords: Animals; Brain; Globins; Immunohistochemistry; In Situ Hybridization; Male; Mice; Mice, Inbred C57BL; Microscopy, Immunoelectron; Nerve Tissue Proteins; Neurons; Nitric Oxide; RNA, Messenger

PY - 2010

Y1 - 2010

N2 - The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb.

AB - The present study aimed at characterizing the anatomical and subcellular localization of cytoglobin (Cygb) and neuroglobin (Ngb) in the mouse brain by use of in situ hybridisation, immunohistochemistry and immunoelectron microscopy. Cygb and Ngb were only found in distinct brain areas and often in the same areas. We found intense staining in the piriform cortex, amygdala, hypothalamus (medial preoptic area, supra chiasmatic nucleus, lateral hypothalamus (LH), ventromedial hypothalamic nucleus, and the arcuate nucleus, habenular nuclei, laterodorsal tegmental nucleus (LDTg), pedunculopontine tegmental nucleus (PPTg), locus coeruleus, nucleus of the solitary tract and the spinal trigeminal nucleus. In addition Cygb is found in the hippocampus, the reticular thalamic nucleus, and the dorsal raphe nucleus; Ngb is found in the sub parabrachial nucleus. Co-localization of Cygb and Ngb is mainly observed in the LDTg and PPTg. Cygb and Ngb were found in cytoplasm, along neurotubuli, in mitochondria and in the nucleus by use of immunoelectron microscopy. Most neuronal nitric oxide synthase (nNOS)-positive neurons were found to co-localize Cygb, although not all nNOS neurones contain Cygb. Ngb co-localize with almost all orexin neurons in the LH. In conclusion the distribution of Cygb and Ngb seems much more restricted and coherent than previously reported. We believe other functions than pure oxygen buffers and neuroprotectants should be considered. The anatomical data indicate a role in NO signalling for Cygb and involvement in sleep-wake cycling for Cygb and Ngb.

U2 - 10.1016/j.brainres.2010.03.056

DO - 10.1016/j.brainres.2010.03.056

M3 - Journal article

C2 - 20331985

SN - 0006-8993

VL - 1331

SP - 58

EP - 73

JO - Brain Research

JF - Brain Research

ER -