Abstract
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Antimicrobial Agents and Chemotherapy |
| Vol/bind | 53 |
| Udgave nummer | 6 |
| Sider (fra-til) | 2483-91 |
| Antal sider | 9 |
| ISSN | 0066-4804 |
| DOI | |
| Status | Udgivet - 1 jun. 2009 |
Bibliografisk note
Keywords: Cystic Fibrosis; DNA Repair; Drug Resistance, Bacterial; Humans; Microbial Sensitivity Tests; Mutation; Neutrophil Activation; Oxidation-Reduction; Oxidative Stress; Pseudomonas aeruginosaAdgang til dokumentet
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I: Antimicrobial Agents and Chemotherapy, Bind 53, Nr. 6, 01.06.2009, s. 2483-91.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Antibiotic resistance in Pseudomonas aeruginosa strains with increased mutation frequency due to inactivation of the DNA oxidative repair system
AU - Mandsberg, L F
AU - Ciofu, O
AU - Kirkby, N
AU - Christiansen, L E
AU - Poulsen, Henrik Enghusen
AU - Høiby, N
AU - Mandsberg, Lotte Frigaard
AU - Ciofu, Oana
AU - Kirkby, N
AU - Christiansen, L E
AU - Poulsen, H E
AU - Høiby, N
N1 - Keywords: Cystic Fibrosis; DNA Repair; Drug Resistance, Bacterial; Humans; Microbial Sensitivity Tests; Mutation; Neutrophil Activation; Oxidation-Reduction; Oxidative Stress; Pseudomonas aeruginosa
PY - 2009/6/1
Y1 - 2009/6/1
N2 - The chronic Pseudomonas aeruginosa infection of the lungs of cystic fibrosis (CF) patients is characterized by the biofilm mode of growth and chronic inflammation dominated by polymorphonuclear leukocytes (PMNs). A high percentage of P. aeruginosa strains show high frequencies of mutations (hypermutators [HP]). P. aeruginosa is exposed to oxygen radicals, both those generated by its own metabolism and especially those released by a large number of PMNs in response to the chronic CF lung infection. Our work therefore focused on the role of the DNA oxidative repair system in the development of HP and antibiotic resistance. We have constructed and characterized mutT, mutY, and mutM mutants in P. aeruginosa strain PAO1. The mutT and mutY mutants showed 28- and 7.5-fold increases in mutation frequencies, respectively, over that for PAO1. These mutators had more oxidative DNA damage (higher levels of 7,8-dihydro-8-oxodeoxyguanosine) than PAO1 after exposure to PMNs, and they developed resistance to antibiotics more frequently. The mechanisms of resistance were increased beta-lactamase production and overexpression of the MexCD-OprJ efflux-pump. Mutations in either the mutT or the mutY gene were found in resistant HP clinical isolates from patients with CF, and complementation with wild-type genes reverted the phenotype. In conclusion, oxidative stress might be involved in the development of resistance to antibiotics. We therefore suggest the possible use of antioxidants for CF patients to prevent the development of antibiotic resistance.
AB - The chronic Pseudomonas aeruginosa infection of the lungs of cystic fibrosis (CF) patients is characterized by the biofilm mode of growth and chronic inflammation dominated by polymorphonuclear leukocytes (PMNs). A high percentage of P. aeruginosa strains show high frequencies of mutations (hypermutators [HP]). P. aeruginosa is exposed to oxygen radicals, both those generated by its own metabolism and especially those released by a large number of PMNs in response to the chronic CF lung infection. Our work therefore focused on the role of the DNA oxidative repair system in the development of HP and antibiotic resistance. We have constructed and characterized mutT, mutY, and mutM mutants in P. aeruginosa strain PAO1. The mutT and mutY mutants showed 28- and 7.5-fold increases in mutation frequencies, respectively, over that for PAO1. These mutators had more oxidative DNA damage (higher levels of 7,8-dihydro-8-oxodeoxyguanosine) than PAO1 after exposure to PMNs, and they developed resistance to antibiotics more frequently. The mechanisms of resistance were increased beta-lactamase production and overexpression of the MexCD-OprJ efflux-pump. Mutations in either the mutT or the mutY gene were found in resistant HP clinical isolates from patients with CF, and complementation with wild-type genes reverted the phenotype. In conclusion, oxidative stress might be involved in the development of resistance to antibiotics. We therefore suggest the possible use of antioxidants for CF patients to prevent the development of antibiotic resistance.
U2 - 10.1128/AAC.00428-08
DO - 10.1128/AAC.00428-08
M3 - Journal article
C2 - 19332676
SN - 0066-4804
VL - 53
SP - 2483
EP - 2491
JO - Antimicrobial Agents and Chemotherapy
JF - Antimicrobial Agents and Chemotherapy
IS - 6
ER -