Antibodies against chromosomal beta-lactamase.

B Giwercman; J W Rasmussen; Oana Ciofu; I Clemmentsen; H Schumacher; N Høiby

Antibodies against chromosomal beta-lactamase.

B Giwercman, J W Rasmussen, Oana Ciofu, I Clemmentsen, H Schumacher, N Høiby

Institut for Kunst- og Kulturvidenskab

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

12 Citationer (Scopus)

Abstract

Udgivelsesdato: 1994-Oct

Originalsprog	Engelsk
Tidsskrift	Antimicrobial Agents and Chemotherapy
Vol/bind	38
Udgave nummer	10
Sider (fra-til)	2306-10
Antal sider	4
ISSN	0066-4804
Status	Udgivet - 1994

Bibliografisk note

Keywords: Adolescent; Adult; Animals; Antibodies, Bacterial; Child; Chromosomes, Bacterial; Cystic Fibrosis; Humans; Immunoglobulin G; Mice; Mice, Inbred BALB C; Middle Aged; Pseudomonas Infections; Pseudomonas aeruginosa; Sputum; beta-Lactamases

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Citationsformater

@article{895f9520ba2211ddae57000ea68e967b,

title = "Antibodies against chromosomal beta-lactamase.",

abstract = "A murine monoclonal anti-chromosomal beta-lactamase antibody was developed and an immunoblotting technique was used to study the presence of serum and sputum antibodies against Pseudomonas aeruginosa chromosomal group 1 beta-lactamase in patients with cystic fibrosis (CF). The serum antibody response was studied with serum samples collected in 1992 from 56 CF patients in a cross-sectional study and with serum samples from 18 CF patients in a longitudinal study. Anti-beta-lactamase immunoglobulin G antibodies were present in all of the serum samples from the patients with chronic bronchopulmonary P. aeruginosa infection (CF + P) but in none of the CF patients with no or intermittent P. aeruginosa infection. Anti-beta-lactamase antibodies were present in serum from CF + P patients after six antipseudomonal courses (median) and correlated with infection with a beta-lactam-resistant strain of P. aeruginosa. The sputum antibody response and the beta-lactamase activity in sputum samples from 14 of the CF + P patients were also studied. beta-lactamase antibodies were present in 10 of these samples. P. aeruginosa strains isolated from these samples were partially derepressed, producing group 1 cephalosporinase. We found a wide range of chromosomal beta-lactamase activity in the sputum samples, with no correlation with basal or induced activity of beta-lactamase expression. The presence of anti-beta-lactamase antibodies in endobronchial sputum could be an important factor in the defense against the infection. On the other hand, immune complexes between the beta-lactamase and corresponding antibodies could play a role in the pathogenesis of bronchopulmonary injury in CF by mediating hyperimmune reactions.",

author = "B Giwercman and Rasmussen, {J W} and Oana Ciofu and I Clemmentsen and H Schumacher and N H{\o}iby",

note = "Keywords: Adolescent; Adult; Animals; Antibodies, Bacterial; Child; Chromosomes, Bacterial; Cystic Fibrosis; Humans; Immunoglobulin G; Mice; Mice, Inbred BALB C; Middle Aged; Pseudomonas Infections; Pseudomonas aeruginosa; Sputum; beta-Lactamases",

year = "1994",

language = "English",

volume = "38",

pages = "2306--10",

journal = "Antimicrobial Agents and Chemotherapy",

issn = "0066-4804",

publisher = "American Society for Microbiology",

number = "10",

}

TY - JOUR

T1 - Antibodies against chromosomal beta-lactamase.

AU - Giwercman, B

AU - Rasmussen, J W

AU - Ciofu, Oana

AU - Clemmentsen, I

AU - Schumacher, H

AU - Høiby, N

N1 - Keywords: Adolescent; Adult; Animals; Antibodies, Bacterial; Child; Chromosomes, Bacterial; Cystic Fibrosis; Humans; Immunoglobulin G; Mice; Mice, Inbred BALB C; Middle Aged; Pseudomonas Infections; Pseudomonas aeruginosa; Sputum; beta-Lactamases

PY - 1994

Y1 - 1994

N2 - A murine monoclonal anti-chromosomal beta-lactamase antibody was developed and an immunoblotting technique was used to study the presence of serum and sputum antibodies against Pseudomonas aeruginosa chromosomal group 1 beta-lactamase in patients with cystic fibrosis (CF). The serum antibody response was studied with serum samples collected in 1992 from 56 CF patients in a cross-sectional study and with serum samples from 18 CF patients in a longitudinal study. Anti-beta-lactamase immunoglobulin G antibodies were present in all of the serum samples from the patients with chronic bronchopulmonary P. aeruginosa infection (CF + P) but in none of the CF patients with no or intermittent P. aeruginosa infection. Anti-beta-lactamase antibodies were present in serum from CF + P patients after six antipseudomonal courses (median) and correlated with infection with a beta-lactam-resistant strain of P. aeruginosa. The sputum antibody response and the beta-lactamase activity in sputum samples from 14 of the CF + P patients were also studied. beta-lactamase antibodies were present in 10 of these samples. P. aeruginosa strains isolated from these samples were partially derepressed, producing group 1 cephalosporinase. We found a wide range of chromosomal beta-lactamase activity in the sputum samples, with no correlation with basal or induced activity of beta-lactamase expression. The presence of anti-beta-lactamase antibodies in endobronchial sputum could be an important factor in the defense against the infection. On the other hand, immune complexes between the beta-lactamase and corresponding antibodies could play a role in the pathogenesis of bronchopulmonary injury in CF by mediating hyperimmune reactions.

AB - A murine monoclonal anti-chromosomal beta-lactamase antibody was developed and an immunoblotting technique was used to study the presence of serum and sputum antibodies against Pseudomonas aeruginosa chromosomal group 1 beta-lactamase in patients with cystic fibrosis (CF). The serum antibody response was studied with serum samples collected in 1992 from 56 CF patients in a cross-sectional study and with serum samples from 18 CF patients in a longitudinal study. Anti-beta-lactamase immunoglobulin G antibodies were present in all of the serum samples from the patients with chronic bronchopulmonary P. aeruginosa infection (CF + P) but in none of the CF patients with no or intermittent P. aeruginosa infection. Anti-beta-lactamase antibodies were present in serum from CF + P patients after six antipseudomonal courses (median) and correlated with infection with a beta-lactam-resistant strain of P. aeruginosa. The sputum antibody response and the beta-lactamase activity in sputum samples from 14 of the CF + P patients were also studied. beta-lactamase antibodies were present in 10 of these samples. P. aeruginosa strains isolated from these samples were partially derepressed, producing group 1 cephalosporinase. We found a wide range of chromosomal beta-lactamase activity in the sputum samples, with no correlation with basal or induced activity of beta-lactamase expression. The presence of anti-beta-lactamase antibodies in endobronchial sputum could be an important factor in the defense against the infection. On the other hand, immune complexes between the beta-lactamase and corresponding antibodies could play a role in the pathogenesis of bronchopulmonary injury in CF by mediating hyperimmune reactions.

M3 - Journal article

C2 - 7840561

SN - 0066-4804

VL - 38

SP - 2306

EP - 2310

JO - Antimicrobial Agents and Chemotherapy

JF - Antimicrobial Agents and Chemotherapy

IS - 10

ER -