Antigen-presenting cells in human cutaneous leishmaniasis due to Leishmania major

A M ElHassan; A Gaafar; T G Theander

Antigen-presenting cells in human cutaneous leishmaniasis due to Leishmania major

A M ElHassan, A Gaafar, T G Theander

Centre for Medical Parasitology

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

29 Citationer (Scopus)

Abstract

Udgivelsesdato: 1995-Mar

Originalsprog	Engelsk
Tidsskrift	Clinical and Experimental Immunology
Vol/bind	99
Udgave nummer	3
Sider (fra-til)	445-53
Antal sider	8
ISSN	0009-9104
Status	Udgivet - 1995

Bibliografisk note

Keywords: Animals; Antigen-Presenting Cells; Antigens, Protozoan; Humans; Immunohistochemistry; Intercellular Adhesion Molecule-1; Leishmania major; Leishmaniasis, Cutaneous; Lymph Nodes; Lymphocyte Function-Associated Antigen-1; Skin; T-Lymphocytes

Citationsformater

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title = "Antigen-presenting cells in human cutaneous leishmaniasis due to Leishmania major",

abstract = "In this study biopsies from skin lesions and draining lymph nodes of patients suffering from cutaneous leishmaniasis caused by Leishmania major were examined by immunohistochemistry, and by light and electron microscopy to identify the types of antigen-presenting cells (APC) and their location. APC, identified morphologically and by their expression of specific cell markers, included Langerhans cells, macrophages, follicular dendritic cells, and interdigitating reticulum cells of the paracortex of lymph nodes. These cells expressed MHC class II antigens and contained Leishmania antigen. Since some keratinocytes and endothelial cells also showed these characteristics, they may also act as APC. By examining tissue samples from skin lesions and draining lymph nodes it was possible to follow the probable route of trafficking of various inflammatory cells between the skin lesion and lymph nodes. Leishmania antigen containing Langerhans cells were found in the epidermis, dermis and the regional lymph nodes. We believe these cells translocate from the epidermis to the dermis, where they take up antigen and migrate to the paracortex of the regional lymph nodes. There they are intimately associated with cells of the paracortex, and could be involved in the generation of Leishmania-specific T memory cells. LFA-1-positive T cells of the CD45RO phenotype were found in the skin lesion. Venular endothelium in the skin lesions expressed intercellular adhesion molecule-1 (ICAM-1), which is the ligand for LFA-1. The migration of lymphocytes from the vascular lumen to the site of inflammation is possibly a result of the interaction of these two adhesion molecules.",

author = "ElHassan, {A M} and A Gaafar and Theander, {T G}",

note = "Keywords: Animals; Antigen-Presenting Cells; Antigens, Protozoan; Humans; Immunohistochemistry; Intercellular Adhesion Molecule-1; Leishmania major; Leishmaniasis, Cutaneous; Lymph Nodes; Lymphocyte Function-Associated Antigen-1; Skin; T-Lymphocytes",

year = "1995",

language = "English",

volume = "99",

pages = "445--53",

journal = "Clinical and Experimental Immunology, Supplement",

issn = "0964-2536",

publisher = "Wiley-Blackwell",

number = "3",

}

TY - JOUR

T1 - Antigen-presenting cells in human cutaneous leishmaniasis due to Leishmania major

AU - ElHassan, A M

AU - Gaafar, A

AU - Theander, T G

N1 - Keywords: Animals; Antigen-Presenting Cells; Antigens, Protozoan; Humans; Immunohistochemistry; Intercellular Adhesion Molecule-1; Leishmania major; Leishmaniasis, Cutaneous; Lymph Nodes; Lymphocyte Function-Associated Antigen-1; Skin; T-Lymphocytes

PY - 1995

Y1 - 1995

N2 - In this study biopsies from skin lesions and draining lymph nodes of patients suffering from cutaneous leishmaniasis caused by Leishmania major were examined by immunohistochemistry, and by light and electron microscopy to identify the types of antigen-presenting cells (APC) and their location. APC, identified morphologically and by their expression of specific cell markers, included Langerhans cells, macrophages, follicular dendritic cells, and interdigitating reticulum cells of the paracortex of lymph nodes. These cells expressed MHC class II antigens and contained Leishmania antigen. Since some keratinocytes and endothelial cells also showed these characteristics, they may also act as APC. By examining tissue samples from skin lesions and draining lymph nodes it was possible to follow the probable route of trafficking of various inflammatory cells between the skin lesion and lymph nodes. Leishmania antigen containing Langerhans cells were found in the epidermis, dermis and the regional lymph nodes. We believe these cells translocate from the epidermis to the dermis, where they take up antigen and migrate to the paracortex of the regional lymph nodes. There they are intimately associated with cells of the paracortex, and could be involved in the generation of Leishmania-specific T memory cells. LFA-1-positive T cells of the CD45RO phenotype were found in the skin lesion. Venular endothelium in the skin lesions expressed intercellular adhesion molecule-1 (ICAM-1), which is the ligand for LFA-1. The migration of lymphocytes from the vascular lumen to the site of inflammation is possibly a result of the interaction of these two adhesion molecules.

AB - In this study biopsies from skin lesions and draining lymph nodes of patients suffering from cutaneous leishmaniasis caused by Leishmania major were examined by immunohistochemistry, and by light and electron microscopy to identify the types of antigen-presenting cells (APC) and their location. APC, identified morphologically and by their expression of specific cell markers, included Langerhans cells, macrophages, follicular dendritic cells, and interdigitating reticulum cells of the paracortex of lymph nodes. These cells expressed MHC class II antigens and contained Leishmania antigen. Since some keratinocytes and endothelial cells also showed these characteristics, they may also act as APC. By examining tissue samples from skin lesions and draining lymph nodes it was possible to follow the probable route of trafficking of various inflammatory cells between the skin lesion and lymph nodes. Leishmania antigen containing Langerhans cells were found in the epidermis, dermis and the regional lymph nodes. We believe these cells translocate from the epidermis to the dermis, where they take up antigen and migrate to the paracortex of the regional lymph nodes. There they are intimately associated with cells of the paracortex, and could be involved in the generation of Leishmania-specific T memory cells. LFA-1-positive T cells of the CD45RO phenotype were found in the skin lesion. Venular endothelium in the skin lesions expressed intercellular adhesion molecule-1 (ICAM-1), which is the ligand for LFA-1. The migration of lymphocytes from the vascular lumen to the site of inflammation is possibly a result of the interaction of these two adhesion molecules.

M3 - Journal article

C2 - 7882568

VL - 99

SP - 445

EP - 453

JO - Clinical and Experimental Immunology, Supplement

JF - Clinical and Experimental Immunology, Supplement

SN - 0964-2536

IS - 3

ER -