Antimicrobial Activity of Sertraline on Listeria monocytogenes

Yidi Wang; Lili Li; Pingyao Cai; Rikke Heidemann Olsen; Shuai Peng; Hecheng Meng

doi:10.3390/ijms24054678

Antimicrobial Activity of Sertraline on Listeria monocytogenes

Yidi Wang, Lili Li, Pingyao Cai, Rikke Heidemann Olsen, Shuai Peng, Hecheng Meng^*

^*Corresponding author af dette arbejde

Sektion, Veterinary Clinical Microbiology

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

4 Citationer (Scopus)

Abstract

We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline against L. monocytogenes were in the range of 16–32 μg/mL and 64 μg/mL, respectively. Sertraline-dependent damage of the cell membrane and a decrease in intracellular ATP and pH_in in L. monocytogenes were observed. In addition, sertraline reduced the biofilm formation efficiency of the L. monocytogenes strains. Importantly, low concentrations (0.1 μg/mL and 1 μg/mL) of sertraline significantly down-regulated the expression levels of various L. monocytogens virulence genes (prfA, actA, degU, flaA, sigB, ltrC and sufS). These results collectively suggest a role of sertraline for the control of L. monocytogenes in the food industry.

Originalsprog	Engelsk
Artikelnummer	4678
Tidsskrift	International Journal of Molecular Sciences
Vol/bind	24
Udgave nummer	5
ISSN	1661-6596
DOI	https://doi.org/10.3390/ijms24054678
Status	Udgivet - 2023

Bibliografisk note

Funding Information:
This work was supported by National Natural Science Foundation (grant number 31901789, 32001796) and Basic Research Project of Guangzhou (grant number: 202002030630020049).

Publisher Copyright:
© 2023 by the authors.

Adgang til dokumentet

10.3390/ijms24054678Licens: CC BY

Citationsformater

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title = "Antimicrobial Activity of Sertraline on Listeria monocytogenes",

abstract = "We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline against L. monocytogenes were in the range of 16–32 μg/mL and 64 μg/mL, respectively. Sertraline-dependent damage of the cell membrane and a decrease in intracellular ATP and pHin in L. monocytogenes were observed. In addition, sertraline reduced the biofilm formation efficiency of the L. monocytogenes strains. Importantly, low concentrations (0.1 μg/mL and 1 μg/mL) of sertraline significantly down-regulated the expression levels of various L. monocytogens virulence genes (prfA, actA, degU, flaA, sigB, ltrC and sufS). These results collectively suggest a role of sertraline for the control of L. monocytogenes in the food industry.",

keywords = "antimicrobial activity, biofilm, Listeria monocytogenes, sertraline, virulence",

author = "Yidi Wang and Lili Li and Pingyao Cai and Olsen, {Rikke Heidemann} and Shuai Peng and Hecheng Meng",

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TY - JOUR

T1 - Antimicrobial Activity of Sertraline on Listeria monocytogenes

AU - Wang, Yidi

AU - Li, Lili

AU - Cai, Pingyao

AU - Olsen, Rikke Heidemann

AU - Peng, Shuai

AU - Meng, Hecheng

PY - 2023

Y1 - 2023

N2 - We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline against L. monocytogenes were in the range of 16–32 μg/mL and 64 μg/mL, respectively. Sertraline-dependent damage of the cell membrane and a decrease in intracellular ATP and pHin in L. monocytogenes were observed. In addition, sertraline reduced the biofilm formation efficiency of the L. monocytogenes strains. Importantly, low concentrations (0.1 μg/mL and 1 μg/mL) of sertraline significantly down-regulated the expression levels of various L. monocytogens virulence genes (prfA, actA, degU, flaA, sigB, ltrC and sufS). These results collectively suggest a role of sertraline for the control of L. monocytogenes in the food industry.

AB - We explored the antimicrobial activity of sertraline on Listeria monocytogenes and further investigated the effects of sertraline on biofilm formation and the virulence gene expression of L. monocytogenes. The minimum inhibitory concentration and minimum bactericidal concentration for sertraline against L. monocytogenes were in the range of 16–32 μg/mL and 64 μg/mL, respectively. Sertraline-dependent damage of the cell membrane and a decrease in intracellular ATP and pHin in L. monocytogenes were observed. In addition, sertraline reduced the biofilm formation efficiency of the L. monocytogenes strains. Importantly, low concentrations (0.1 μg/mL and 1 μg/mL) of sertraline significantly down-regulated the expression levels of various L. monocytogens virulence genes (prfA, actA, degU, flaA, sigB, ltrC and sufS). These results collectively suggest a role of sertraline for the control of L. monocytogenes in the food industry.

KW - antimicrobial activity

KW - biofilm

KW - Listeria monocytogenes

KW - sertraline

KW - virulence

U2 - 10.3390/ijms24054678

DO - 10.3390/ijms24054678

M3 - Journal article

C2 - 36902108

AN - SCOPUS:85149816779

VL - 24

JO - International Journal of Molecular Sciences (Online)

JF - International Journal of Molecular Sciences (Online)

SN - 1661-6596

IS - 5

M1 - 4678

ER -