Apparent involvement of purines in the control of expression of Salmonella typhimurium pyr genes: analysis of a leaky guaB mutant resistant to pyrimidine analogs.

Kaj Frank Jensen

Apparent involvement of purines in the control of expression of Salmonella typhimurium pyr genes: analysis of a leaky guaB mutant resistant to pyrimidine analogs.

Kaj Frank Jensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

28 Citationer (Scopus)

Abstract

Udgivelsesdato: 1979 June

Originalsprog	Engelsk
Tidsskrift	Journal of Bacteriology
Vol/bind	138
Udgave nummer	3
Sider (fra-til)	731-738
ISSN	0021-9193
Status	Udgivet - 1979

Adgang til dokumentet

http://jb.asm.org/cgi/reprint/138/3/731

Citationsformater

@article{4dcc1850847a11dcbee902004c4f4f50,

title = "Apparent involvement of purines in the control of expression of Salmonella typhimurium pyr genes: analysis of a leaky guaB mutant resistant to pyrimidine analogs.",

abstract = "A leaky guaB mutant of Salmonella typhimurium LT-2 was obtained during a selection for mutants resistant to a combination of the two pyrimidine analogs, 5-fluorouracil and 5-fluorouridine. In the absence of exogenous guanine compounds, the growth rate of this mutant is limited by the endogenous supply of guanine nucleotides due to a defective inosine 5'-monophosphate dehydrogenase. Under these conditions the guanosine 5'-triphosphate pool is about 20% of normal, the cytidine 5'-triphosphate pool is reduced to below 60%, and the uridine 5'-triphosphate pool is slightly elevated. Simultaneously, levels of the pyrimidine biosynthetic enzymes are abnormal: aspartate transcarbamylase, orotate phosphoribosyltransferase, and orotidylic acid decarboxylase levels are increased 4-, 11-, and 3-fold, respectively. Levels of dihydroorotase and dihydroorotate dehydrogenase are decreased to 10 and 20%, respectively. The pyrimidine metabolism of the guaB mutant is restored completely by addition of guanine (or xanthine) to the growth medium. The data indicate purine nucleotide involvement in the regulation of expression of the pyr genes of S. typhimurium.",

author = "Jensen, {Kaj Frank}",

year = "1979",

language = "English",

volume = "138",

pages = "731--738",

journal = "Journal of Bacteriology",

issn = "0021-9193",

publisher = "American Society for Microbiology",

number = "3",

}

TY - JOUR

T1 - Apparent involvement of purines in the control of expression of Salmonella typhimurium pyr genes: analysis of a leaky guaB mutant resistant to pyrimidine analogs.

AU - Jensen, Kaj Frank

PY - 1979

Y1 - 1979

N2 - A leaky guaB mutant of Salmonella typhimurium LT-2 was obtained during a selection for mutants resistant to a combination of the two pyrimidine analogs, 5-fluorouracil and 5-fluorouridine. In the absence of exogenous guanine compounds, the growth rate of this mutant is limited by the endogenous supply of guanine nucleotides due to a defective inosine 5'-monophosphate dehydrogenase. Under these conditions the guanosine 5'-triphosphate pool is about 20% of normal, the cytidine 5'-triphosphate pool is reduced to below 60%, and the uridine 5'-triphosphate pool is slightly elevated. Simultaneously, levels of the pyrimidine biosynthetic enzymes are abnormal: aspartate transcarbamylase, orotate phosphoribosyltransferase, and orotidylic acid decarboxylase levels are increased 4-, 11-, and 3-fold, respectively. Levels of dihydroorotase and dihydroorotate dehydrogenase are decreased to 10 and 20%, respectively. The pyrimidine metabolism of the guaB mutant is restored completely by addition of guanine (or xanthine) to the growth medium. The data indicate purine nucleotide involvement in the regulation of expression of the pyr genes of S. typhimurium.

AB - A leaky guaB mutant of Salmonella typhimurium LT-2 was obtained during a selection for mutants resistant to a combination of the two pyrimidine analogs, 5-fluorouracil and 5-fluorouridine. In the absence of exogenous guanine compounds, the growth rate of this mutant is limited by the endogenous supply of guanine nucleotides due to a defective inosine 5'-monophosphate dehydrogenase. Under these conditions the guanosine 5'-triphosphate pool is about 20% of normal, the cytidine 5'-triphosphate pool is reduced to below 60%, and the uridine 5'-triphosphate pool is slightly elevated. Simultaneously, levels of the pyrimidine biosynthetic enzymes are abnormal: aspartate transcarbamylase, orotate phosphoribosyltransferase, and orotidylic acid decarboxylase levels are increased 4-, 11-, and 3-fold, respectively. Levels of dihydroorotase and dihydroorotate dehydrogenase are decreased to 10 and 20%, respectively. The pyrimidine metabolism of the guaB mutant is restored completely by addition of guanine (or xanthine) to the growth medium. The data indicate purine nucleotide involvement in the regulation of expression of the pyr genes of S. typhimurium.

M3 - Journal article

SN - 0021-9193

VL - 138

SP - 731

EP - 738

JO - Journal of Bacteriology

JF - Journal of Bacteriology

IS - 3

ER -