Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid.

Jing Yang; Henry Klassen; Mette Pries; Wei Wang; Mogens H Nissen

doi:10.1634/stemcells.2006-0103

Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid.

Jing Yang, Henry Klassen, Mette Pries, Wei Wang, Mogens H Nissen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

20 Citationer (Scopus)

Abstract

Udgivelsesdato: 2006-Dec

Originalsprog	Engelsk
Tidsskrift	Stem Cells
Vol/bind	24
Udgave nummer	12
Sider (fra-til)	2766-75
Antal sider	9
ISSN	1066-5099
DOI	https://doi.org/10.1634/stemcells.2006-0103
Status	Udgivet - 2006

Bibliografisk note

Keywords: Animals; Aqueous Humor; Ascorbic Acid; Biological Markers; Cell Proliferation; Cells, Cultured; Chromatography, Gel; Culture Media, Serum-Free; Epidermal Growth Factor; Female; Freezing; Molecular Weight; Rats; Rats, Sprague-Dawley; Retina; Spectrophotometry, Ultraviolet; Stem Cells; Swine

Adgang til dokumentet

10.1634/stemcells.2006-0103

Citationsformater

@article{5e5214805ed711dd8d9f000ea68e967b,

title = "Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid.",

abstract = "Aqueous humor has been shown to influence the proliferation of various ocular cell types, but the effect on immature retinal cells is not known. Here, the effect of pig aqueous humor on the proliferation of rat retinal precursor cells (RPCs) was investigated. RPCs were prepared from embryonic day 19 Sprague-Dawley rats and cultured in the presence or absence of aqueous humor from healthy pigs along with a medium consisting of Dulbecco's modified Eagle's medium:Ham's F-12 medium, N2 supplement, and epidermal growth factor. Proliferation was quantified by [(3)H]thymidine incorporation under different treatment conditions, and any associated morphological changes were noted. Potential active components of porcine aqueous humor were partially characterized by gel filtration chromatography, and the effect on RPC proliferation was determined. Results showed that adding 20% aqueous humor increased [(3)H]thymidine incorporation by as much as 317%, as compared with controls. Aqueous supplementation also increased both the number and size of RPC spherical aggregates ({"}spheres{"}) over the first 4 days, consistent with increased proliferative activity. Using gel filtration and the in vitro proliferation assay, the growth-promoting activity of aqueous humor was localized to two different molecular mass ranges, namely, around 30 kDa and less than 1 kDa. Ascorbic acid was present in the lower molecular mass fraction, and use of this molecule reproduced some, but not all, of the proliferative activity present in aqueous humor. These results highlight the potential role of soluble factors present in the cellular microenvironment with respect to modulation of endogenous progenitor cell activity.",

author = "Jing Yang and Henry Klassen and Mette Pries and Wei Wang and Nissen, {Mogens H}",

note = "Keywords: Animals; Aqueous Humor; Ascorbic Acid; Biological Markers; Cell Proliferation; Cells, Cultured; Chromatography, Gel; Culture Media, Serum-Free; Epidermal Growth Factor; Female; Freezing; Molecular Weight; Rats; Rats, Sprague-Dawley; Retina; Spectrophotometry, Ultraviolet; Stem Cells; Swine",

year = "2006",

doi = "10.1634/stemcells.2006-0103",

language = "English",

volume = "24",

pages = "2766--75",

journal = "Stem Cells",

issn = "1066-5099",

publisher = "AlphaMed Press, Inc.",

number = "12",

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TY - JOUR

T1 - Aqueous humor enhances the proliferation of rat retinal precursor cells in culture, and this effect is partially reproduced by ascorbic acid.

AU - Yang, Jing

AU - Klassen, Henry

AU - Pries, Mette

AU - Wang, Wei

AU - Nissen, Mogens H

N1 - Keywords: Animals; Aqueous Humor; Ascorbic Acid; Biological Markers; Cell Proliferation; Cells, Cultured; Chromatography, Gel; Culture Media, Serum-Free; Epidermal Growth Factor; Female; Freezing; Molecular Weight; Rats; Rats, Sprague-Dawley; Retina; Spectrophotometry, Ultraviolet; Stem Cells; Swine

PY - 2006

Y1 - 2006

N2 - Aqueous humor has been shown to influence the proliferation of various ocular cell types, but the effect on immature retinal cells is not known. Here, the effect of pig aqueous humor on the proliferation of rat retinal precursor cells (RPCs) was investigated. RPCs were prepared from embryonic day 19 Sprague-Dawley rats and cultured in the presence or absence of aqueous humor from healthy pigs along with a medium consisting of Dulbecco's modified Eagle's medium:Ham's F-12 medium, N2 supplement, and epidermal growth factor. Proliferation was quantified by [(3)H]thymidine incorporation under different treatment conditions, and any associated morphological changes were noted. Potential active components of porcine aqueous humor were partially characterized by gel filtration chromatography, and the effect on RPC proliferation was determined. Results showed that adding 20% aqueous humor increased [(3)H]thymidine incorporation by as much as 317%, as compared with controls. Aqueous supplementation also increased both the number and size of RPC spherical aggregates ("spheres") over the first 4 days, consistent with increased proliferative activity. Using gel filtration and the in vitro proliferation assay, the growth-promoting activity of aqueous humor was localized to two different molecular mass ranges, namely, around 30 kDa and less than 1 kDa. Ascorbic acid was present in the lower molecular mass fraction, and use of this molecule reproduced some, but not all, of the proliferative activity present in aqueous humor. These results highlight the potential role of soluble factors present in the cellular microenvironment with respect to modulation of endogenous progenitor cell activity.

AB - Aqueous humor has been shown to influence the proliferation of various ocular cell types, but the effect on immature retinal cells is not known. Here, the effect of pig aqueous humor on the proliferation of rat retinal precursor cells (RPCs) was investigated. RPCs were prepared from embryonic day 19 Sprague-Dawley rats and cultured in the presence or absence of aqueous humor from healthy pigs along with a medium consisting of Dulbecco's modified Eagle's medium:Ham's F-12 medium, N2 supplement, and epidermal growth factor. Proliferation was quantified by [(3)H]thymidine incorporation under different treatment conditions, and any associated morphological changes were noted. Potential active components of porcine aqueous humor were partially characterized by gel filtration chromatography, and the effect on RPC proliferation was determined. Results showed that adding 20% aqueous humor increased [(3)H]thymidine incorporation by as much as 317%, as compared with controls. Aqueous supplementation also increased both the number and size of RPC spherical aggregates ("spheres") over the first 4 days, consistent with increased proliferative activity. Using gel filtration and the in vitro proliferation assay, the growth-promoting activity of aqueous humor was localized to two different molecular mass ranges, namely, around 30 kDa and less than 1 kDa. Ascorbic acid was present in the lower molecular mass fraction, and use of this molecule reproduced some, but not all, of the proliferative activity present in aqueous humor. These results highlight the potential role of soluble factors present in the cellular microenvironment with respect to modulation of endogenous progenitor cell activity.

U2 - 10.1634/stemcells.2006-0103

DO - 10.1634/stemcells.2006-0103

M3 - Journal article

C2 - 16902197

SN - 1066-5099

VL - 24

SP - 2766

EP - 2775

JO - Stem Cells

JF - Stem Cells

IS - 12

ER -