Azone® decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect

Emil Meng-Lund; Jette Jacobsen; Liang Jin; Christian Janfelt; René Holm; Anette Müllertz; Joseph A Nicolazzo

doi:10.1002/jps.23877

Azone® decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect

Emil Meng-Lund, Jette Jacobsen, Liang Jin, Christian Janfelt, René Holm, Anette Müllertz, Joseph A Nicolazzo

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

13 Citationer (Scopus)

Abstract

The purpose of this study was to examine concentration-dependent effects of Azone® (AZ) on the buccal absorption of diazepam (DIAZ). Porcine buccal mucosa was placed in modified Ussing chambers and pretreated with 10 μL of 0%, 5%, 20%, and 50% (w/v) AZ in ethanol. DIAZ was administered to the donor chamber either in solution or a chitosan-based gel. The donor chamber disappearance, receptor chamber appearance, and tissue retention of DIAZ were monitored over 2 h by HPLC, with AZ tissue disposition also measured by liquid chromatography-mass spectrometry profiling of tissue cryosections. DIAZ steady-state flux values significantly (p

Originalsprog	Engelsk
Artikelnummer	103
Tidsskrift	Journal of Pharmaceutical Sciences
Vol/bind	103
Udgave nummer	4
Sider (fra-til)	1133-1141
ISSN	0022-3549
DOI	https://doi.org/10.1002/jps.23877
Status	Udgivet - 15 feb. 2014

Adgang til dokumentet

10.1002/jps.23877

Citationsformater

Azone® decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect. / Meng-Lund, Emil; Jacobsen, Jette; Jin, Liang; Janfelt, Christian; Holm, René; Müllertz, Anette; Nicolazzo, Joseph A.

I: Journal of Pharmaceutical Sciences, Bind 103, Nr. 4, 103, 15.02.2014, s. 1133-1141.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Azone{\textregistered} decreases the buccal mucosal permeation of Diazepam in a concentration-dependent manner via a reservoir effect",

abstract = "The purpose of this study was to examine concentration-dependent effects of Azone{\textregistered} (AZ) on the buccal absorption of diazepam (DIAZ). Porcine buccal mucosa was placed in modified Ussing chambers and pretreated with 10 μL of 0%, 5%, 20%, and 50% (w/v) AZ in ethanol. DIAZ was administered to the donor chamber either in solution or a chitosan-based gel. The donor chamber disappearance, receptor chamber appearance, and tissue retention of DIAZ were monitored over 2 h by HPLC, with AZ tissue disposition also measured by liquid chromatography-mass spectrometry profiling of tissue cryosections. DIAZ steady-state flux values significantly (p ",

author = "Emil Meng-Lund and Jette Jacobsen and Liang Jin and Christian Janfelt and Ren{\'e} Holm and Anette M{\"u}llertz and Nicolazzo, {Joseph A}",

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AU - Meng-Lund, Emil

AU - Jacobsen, Jette

AU - Jin, Liang

AU - Janfelt, Christian

AU - Holm, René

AU - Müllertz, Anette

AU - Nicolazzo, Joseph A

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AB - The purpose of this study was to examine concentration-dependent effects of Azone® (AZ) on the buccal absorption of diazepam (DIAZ). Porcine buccal mucosa was placed in modified Ussing chambers and pretreated with 10 μL of 0%, 5%, 20%, and 50% (w/v) AZ in ethanol. DIAZ was administered to the donor chamber either in solution or a chitosan-based gel. The donor chamber disappearance, receptor chamber appearance, and tissue retention of DIAZ were monitored over 2 h by HPLC, with AZ tissue disposition also measured by liquid chromatography-mass spectrometry profiling of tissue cryosections. DIAZ steady-state flux values significantly (p

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DO - 10.1002/jps.23877

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JO - Journal of Pharmaceutical Sciences

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