Bacterial diversity and community structure of a sub-surface aquifer exposed to realistic low herbicide concentrations

Julia R. de Lipthay; Kaare Johnsen; H.-J. Albrechtsen; Per Rosenberg; Jens Aamand

doi:10.1016/j.femsec.2004.02.007

Bacterial diversity and community structure of a sub-surface aquifer exposed to realistic low herbicide concentrations

Julia R. de Lipthay, Kaare Johnsen, H.-J. Albrechtsen, Per Rosenberg, Jens Aamand

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

41 Citationer (Scopus)

Abstract

Udgivelsesdato: July 2004

Originalsprog	Engelsk
Tidsskrift	FEMS Microbiology Ecology
Vol/bind	49
Udgave nummer	1
Sider (fra-til)	59-69
ISSN	0168-6496
DOI	https://doi.org/10.1016/j.femsec.2004.02.007
Status	Udgivet - 2004

Bibliografisk note

KEYWORDS
Microbial diversity • Microbial community structure • Herbicide • In situ exposure • Sub-surface aquifer

Adgang til dokumentet

10.1016/j.femsec.2004.02.007

Citationsformater

@article{acbddb9074c311dbbee902004c4f4f50,

title = "Bacterial diversity and community structure of a sub-surface aquifer exposed to realistic low herbicide concentrations",

abstract = "An increasing number of herbicides are found in our groundwater environments. This underlines the need for examining the effects of herbicide exposure on the indigenous groundwater microbial communities, as microbial degradation is the major process responsible for the complete removal of most contaminants. We examined the effect of in situ exposure to realistic low concentrations of herbicides on the microbial diversity and community structure of sub-surface sediments from a shallow aquifer near Vejen (Denmark). Three different community analyses were performed: colony morphology typing, sole-carbon source utilisation in Biolog{\textregistered}EcoPlates, and denaturing gradient gel electrophoresis. Cluster analysis demonstrated that the microbial communities of those aquifer sediments that acclimated to the herbicide exposure also had similar community structure. This observation was concurrent for all three community analyses. In contrast, no significant effect was found on the bacterial diversity, except for the culturable fraction where a significantly increased richness and Shannon index was found in the herbicide acclimated sediments. The results of this study show that in situ exposure of sub-surface aquifers to realistic low concentrations of herbicides may alter the overall structure of a natural bacterial community, although significant effects on the genetic diversity and carbon substrate usage cannot be detected. The observed impact was probably due to indirect effects. In future investigations, the inclusion of methods that specifically detect relevant microbial sub-populations and functional genes is therefore recommended.",

author = "Lipthay, {Julia R. de} and Kaare Johnsen and H.-J. Albrechtsen and Per Rosenberg and Jens Aamand",

note = "KEYWORDS Microbial diversity • Microbial community structure • Herbicide • In situ exposure • Sub-surface aquifer",

year = "2004",

doi = "10.1016/j.femsec.2004.02.007",

language = "English",

volume = "49",

pages = "59--69",

journal = "FEMS Microbiology Ecology",

issn = "0168-6496",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Bacterial diversity and community structure of a sub-surface aquifer exposed to realistic low herbicide concentrations

AU - Lipthay, Julia R. de

AU - Johnsen, Kaare

AU - Albrechtsen, H.-J.

AU - Rosenberg, Per

AU - Aamand, Jens

N1 - KEYWORDS Microbial diversity • Microbial community structure • Herbicide • In situ exposure • Sub-surface aquifer

PY - 2004

Y1 - 2004

N2 - An increasing number of herbicides are found in our groundwater environments. This underlines the need for examining the effects of herbicide exposure on the indigenous groundwater microbial communities, as microbial degradation is the major process responsible for the complete removal of most contaminants. We examined the effect of in situ exposure to realistic low concentrations of herbicides on the microbial diversity and community structure of sub-surface sediments from a shallow aquifer near Vejen (Denmark). Three different community analyses were performed: colony morphology typing, sole-carbon source utilisation in Biolog®EcoPlates, and denaturing gradient gel electrophoresis. Cluster analysis demonstrated that the microbial communities of those aquifer sediments that acclimated to the herbicide exposure also had similar community structure. This observation was concurrent for all three community analyses. In contrast, no significant effect was found on the bacterial diversity, except for the culturable fraction where a significantly increased richness and Shannon index was found in the herbicide acclimated sediments. The results of this study show that in situ exposure of sub-surface aquifers to realistic low concentrations of herbicides may alter the overall structure of a natural bacterial community, although significant effects on the genetic diversity and carbon substrate usage cannot be detected. The observed impact was probably due to indirect effects. In future investigations, the inclusion of methods that specifically detect relevant microbial sub-populations and functional genes is therefore recommended.

AB - An increasing number of herbicides are found in our groundwater environments. This underlines the need for examining the effects of herbicide exposure on the indigenous groundwater microbial communities, as microbial degradation is the major process responsible for the complete removal of most contaminants. We examined the effect of in situ exposure to realistic low concentrations of herbicides on the microbial diversity and community structure of sub-surface sediments from a shallow aquifer near Vejen (Denmark). Three different community analyses were performed: colony morphology typing, sole-carbon source utilisation in Biolog®EcoPlates, and denaturing gradient gel electrophoresis. Cluster analysis demonstrated that the microbial communities of those aquifer sediments that acclimated to the herbicide exposure also had similar community structure. This observation was concurrent for all three community analyses. In contrast, no significant effect was found on the bacterial diversity, except for the culturable fraction where a significantly increased richness and Shannon index was found in the herbicide acclimated sediments. The results of this study show that in situ exposure of sub-surface aquifers to realistic low concentrations of herbicides may alter the overall structure of a natural bacterial community, although significant effects on the genetic diversity and carbon substrate usage cannot be detected. The observed impact was probably due to indirect effects. In future investigations, the inclusion of methods that specifically detect relevant microbial sub-populations and functional genes is therefore recommended.

U2 - 10.1016/j.femsec.2004.02.007

DO - 10.1016/j.femsec.2004.02.007

M3 - Journal article

C2 - 19712384

SN - 0168-6496

VL - 49

SP - 59

EP - 69

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

IS - 1

ER -