Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates.

Cornelia Steinhauer; Anton Ressine; György Marko-Varga; Thomas Laurell; Carl A K Borrebaeck; Christer Wingren

doi:10.1016/j.ab.2004.10.036

Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates.

Cornelia Steinhauer, Anton Ressine, György Marko-Varga, Thomas Laurell, Carl A K Borrebaeck, Christer Wingren

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

74 Citationer (Scopus)

Abstract

Udgivelsesdato: 2005-Jun-15

Originalsprog	Engelsk
Tidsskrift	Analytical Biochemistry
Vol/bind	341
Udgave nummer	2
Sider (fra-til)	204-13
Antal sider	9
ISSN	0003-2697
DOI	https://doi.org/10.1016/j.ab.2004.10.036
Status	Udgivet - 2005
Udgivet eksternt	Ja

Bibliografisk note

Keywords: Antibodies; Collodion; Humans; Organosilicon Compounds; Protein Array Analysis; Proteomics; Silicon

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10.1016/j.ab.2004.10.036

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Citationsformater

@article{5b39e3f057c411dd8d9f000ea68e967b,

title = "Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates.",

abstract = "Major efforts to develop antibody microarray technology to enable global proteome analysis to be performed in a facile manner are under way. In this process, the design and the properties of the substrate will play crucial roles. In the present study, we have developed novel, highly biocompatible solid supports for microarrays, using adsorbed recombinant human single-framework antibody fragments as probes. Several silicon-based supports, including planar silicon, micro- and macroporous silicon, and nitrocellulose-coated variants thereof, were designed and evaluated in a stepwise procedure. The surfaces were scored based on biocompatibility and probe binding capacity as judged by spot morphology, signal intensities, signal to noise ratios, dynamic range, sensitivity, and reproducibility. A set of five commercially available substrates, selected to represent a set of supports providing different surface and coupling chemistries, was used as reference surfaces. The results showed that several well-performing silicon-based supports could be designed; in particular, a nitrocellulose-coated macroporous variant, MAP3-NC7, received the highest scores. In comparison, MAP3-NC7 displayed properties equal to or better than those of the reference substrates. Taken together, designed surfaces based on silicon can undoubtedly meet the requirements of the next generation of solid supports for antibody microarrays.",

author = "Cornelia Steinhauer and Anton Ressine and Gy{\"o}rgy Marko-Varga and Thomas Laurell and Borrebaeck, {Carl A K} and Christer Wingren",

note = "Keywords: Antibodies; Collodion; Humans; Organosilicon Compounds; Protein Array Analysis; Proteomics; Silicon",

year = "2005",

doi = "10.1016/j.ab.2004.10.036",

language = "English",

volume = "341",

pages = "204--13",

journal = "Analytical Biochemistry",

issn = "0003-2697",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Biocompatibility of surfaces for antibody microarrays: design of macroporous silicon substrates.

AU - Steinhauer, Cornelia

AU - Ressine, Anton

AU - Marko-Varga, György

AU - Laurell, Thomas

AU - Borrebaeck, Carl A K

AU - Wingren, Christer

N1 - Keywords: Antibodies; Collodion; Humans; Organosilicon Compounds; Protein Array Analysis; Proteomics; Silicon

PY - 2005

Y1 - 2005

N2 - Major efforts to develop antibody microarray technology to enable global proteome analysis to be performed in a facile manner are under way. In this process, the design and the properties of the substrate will play crucial roles. In the present study, we have developed novel, highly biocompatible solid supports for microarrays, using adsorbed recombinant human single-framework antibody fragments as probes. Several silicon-based supports, including planar silicon, micro- and macroporous silicon, and nitrocellulose-coated variants thereof, were designed and evaluated in a stepwise procedure. The surfaces were scored based on biocompatibility and probe binding capacity as judged by spot morphology, signal intensities, signal to noise ratios, dynamic range, sensitivity, and reproducibility. A set of five commercially available substrates, selected to represent a set of supports providing different surface and coupling chemistries, was used as reference surfaces. The results showed that several well-performing silicon-based supports could be designed; in particular, a nitrocellulose-coated macroporous variant, MAP3-NC7, received the highest scores. In comparison, MAP3-NC7 displayed properties equal to or better than those of the reference substrates. Taken together, designed surfaces based on silicon can undoubtedly meet the requirements of the next generation of solid supports for antibody microarrays.

AB - Major efforts to develop antibody microarray technology to enable global proteome analysis to be performed in a facile manner are under way. In this process, the design and the properties of the substrate will play crucial roles. In the present study, we have developed novel, highly biocompatible solid supports for microarrays, using adsorbed recombinant human single-framework antibody fragments as probes. Several silicon-based supports, including planar silicon, micro- and macroporous silicon, and nitrocellulose-coated variants thereof, were designed and evaluated in a stepwise procedure. The surfaces were scored based on biocompatibility and probe binding capacity as judged by spot morphology, signal intensities, signal to noise ratios, dynamic range, sensitivity, and reproducibility. A set of five commercially available substrates, selected to represent a set of supports providing different surface and coupling chemistries, was used as reference surfaces. The results showed that several well-performing silicon-based supports could be designed; in particular, a nitrocellulose-coated macroporous variant, MAP3-NC7, received the highest scores. In comparison, MAP3-NC7 displayed properties equal to or better than those of the reference substrates. Taken together, designed surfaces based on silicon can undoubtedly meet the requirements of the next generation of solid supports for antibody microarrays.

U2 - 10.1016/j.ab.2004.10.036

DO - 10.1016/j.ab.2004.10.036

M3 - Journal article

C2 - 15907865

SN - 0003-2697

VL - 341

SP - 204

EP - 213

JO - Analytical Biochemistry

JF - Analytical Biochemistry

IS - 2

ER -