Bioconjugation of Supramolecular Metallacages to Integrin Ligands for Targeted Delivery of Cisplatin

Jiaying Han, Andreas F B Räder, Florian Reichart, Brech Aikman, Margot N Wenzel, Ben Woods, Michael Weinmüller, Beatrice S Ludwig, Stefan Stürup, Geny M M Groothuis, Hjalmar P Permentier, Rainer Bischoff, Horst Kessler, Peter Horvatovich, Angela Casini

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44 Citationer (Scopus)

Abstract

Cisplatin occupies a crucial role in the treatment of various malignant tumors. However, its efficacy and applicability are heavily restricted by severe systemic toxicities and drug resistance. Our study exploits the active targeting of supramolecular metallacages to enhance the activity of cisplatin in cancer cells while reducing its toxicity. Thus, Pd2L4 cages (L = ligand) have been conjugated to four integrin ligands with different binding affinity and selectivity. Cage formation and encapsulation of cisplatin was proven by NMR spectroscopy. Upon encapsulation, cisplatin showed increased cytotoxicity in vitro, in melanoma A375 cells overexpressing αvβ3 integrins. Moreover, ex vivo studies in tissue slices indicated reduced toxicity toward healthy liver and kidney tissues for cage-encapsulated cisplatin. Analysis of metal content by ICP-MS demonstrated that the encapsulated drug is less accumulated in these organs compared to the "free" cisplatin.

OriginalsprogEngelsk
TidsskriftBioconjugate Chemistry
Vol/bind29
Udgave nummer11
Sider (fra-til)3856-3865
Antal sider10
ISSN1043-1802
DOI
StatusUdgivet - 21 nov. 2018

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