Blood cell gene expression profiling in subjects with aggressive periodontitis and chronic arthritis

Lars K Sørensen; Anne Havemose Poulsen; Søren U Sønder; Klaus Bendtzen; Palle Holmstrup

doi:10.1902/jop.2008.070309

Blood cell gene expression profiling in subjects with aggressive periodontitis and chronic arthritis

Lars K Sørensen, Anne Havemose Poulsen, Søren U Sønder, Klaus Bendtzen, Palle Holmstrup

Institut for Klinisk Medicin

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

36 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008

Originalsprog	Engelsk
Tidsskrift	Journal of Periodontology
Vol/bind	79
Udgave nummer	3
Sider (fra-til)	477-485
Antal sider	9
ISSN	0022-3492
DOI	https://doi.org/10.1902/jop.2008.070309
Status	Udgivet - 2008

Adgang til dokumentet

10.1902/jop.2008.070309

Citationsformater

@article{68a030d0f69d11ddbf70000ea68e967b,

title = "Blood cell gene expression profiling in subjects with aggressive periodontitis and chronic arthritis",

abstract = "BACKGROUND: Microarray analysis of local and peripheral cells in subjects with immune-inflammatory diseases may identify candidate genes associated with these diseases. The present study identified differentially expressed genes in peripheral blood mononuclear cells (PBMCs) from subjects with untreated localized aggressive periodontitis (LAgP) or generalized aggressive periodontitis (GAgP). Differentially expressed genes were validated in groups of subjects with LAgP, GAgP, juvenile idiopathic arthritis (JIA), or rheumatoid arthritis (RA) and controls. METHODS: Candidate genes were identified by gene expression profiling of PBMCs using a microarray system in untreated gender-matched subjects with LAgP (N = 2) or GAgP (N = 3) and controls (N = 2) younger than 35 years of age. The microarray results were validated by real-time reverse transcription-polymerase chain reaction (RT-PCR) using PBMCs from 103 individuals, including groups of subjects with LAgP (N = 18), GAgP (N = 27), JIA (N = 10), or RA (N = 23) and controls (N = 25). RESULTS: Of 53 differentially expressed candidate genes identified in subjects with LAgP, 14 were involved in immune responses and inflammatory processes. Of these, the RT-PCR validation confirmed that Toll-like receptor 2 gene (TLR2) and myomesin 2 gene had a significantly higher expression in subjects with LAgP than in controls. RT-PCR also showed increased expression of TLR2 in subjects with RA. Comparison of subjects with GAgP to controls using microarray analysis identified only three upregulated genes. CONCLUSION: Several genes upregulated in subjects with LAgP were related to immune responses including TLR2 and myomesin 2.",

author = "S{\o}rensen, {Lars K} and Poulsen, {Anne Havemose} and S{\o}nder, {S{\o}ren U} and Klaus Bendtzen and Palle Holmstrup",

year = "2008",

doi = "10.1902/jop.2008.070309",

language = "English",

volume = "79",

pages = "477--485",

journal = "Journal of Periodontology",

issn = "0022-3492",

publisher = "American Academy of Periodontology",

number = "3",

}

TY - JOUR

T1 - Blood cell gene expression profiling in subjects with aggressive periodontitis and chronic arthritis

AU - Sørensen, Lars K

AU - Poulsen, Anne Havemose

AU - Sønder, Søren U

AU - Bendtzen, Klaus

AU - Holmstrup, Palle

PY - 2008

Y1 - 2008

N2 - BACKGROUND: Microarray analysis of local and peripheral cells in subjects with immune-inflammatory diseases may identify candidate genes associated with these diseases. The present study identified differentially expressed genes in peripheral blood mononuclear cells (PBMCs) from subjects with untreated localized aggressive periodontitis (LAgP) or generalized aggressive periodontitis (GAgP). Differentially expressed genes were validated in groups of subjects with LAgP, GAgP, juvenile idiopathic arthritis (JIA), or rheumatoid arthritis (RA) and controls. METHODS: Candidate genes were identified by gene expression profiling of PBMCs using a microarray system in untreated gender-matched subjects with LAgP (N = 2) or GAgP (N = 3) and controls (N = 2) younger than 35 years of age. The microarray results were validated by real-time reverse transcription-polymerase chain reaction (RT-PCR) using PBMCs from 103 individuals, including groups of subjects with LAgP (N = 18), GAgP (N = 27), JIA (N = 10), or RA (N = 23) and controls (N = 25). RESULTS: Of 53 differentially expressed candidate genes identified in subjects with LAgP, 14 were involved in immune responses and inflammatory processes. Of these, the RT-PCR validation confirmed that Toll-like receptor 2 gene (TLR2) and myomesin 2 gene had a significantly higher expression in subjects with LAgP than in controls. RT-PCR also showed increased expression of TLR2 in subjects with RA. Comparison of subjects with GAgP to controls using microarray analysis identified only three upregulated genes. CONCLUSION: Several genes upregulated in subjects with LAgP were related to immune responses including TLR2 and myomesin 2.

AB - BACKGROUND: Microarray analysis of local and peripheral cells in subjects with immune-inflammatory diseases may identify candidate genes associated with these diseases. The present study identified differentially expressed genes in peripheral blood mononuclear cells (PBMCs) from subjects with untreated localized aggressive periodontitis (LAgP) or generalized aggressive periodontitis (GAgP). Differentially expressed genes were validated in groups of subjects with LAgP, GAgP, juvenile idiopathic arthritis (JIA), or rheumatoid arthritis (RA) and controls. METHODS: Candidate genes were identified by gene expression profiling of PBMCs using a microarray system in untreated gender-matched subjects with LAgP (N = 2) or GAgP (N = 3) and controls (N = 2) younger than 35 years of age. The microarray results were validated by real-time reverse transcription-polymerase chain reaction (RT-PCR) using PBMCs from 103 individuals, including groups of subjects with LAgP (N = 18), GAgP (N = 27), JIA (N = 10), or RA (N = 23) and controls (N = 25). RESULTS: Of 53 differentially expressed candidate genes identified in subjects with LAgP, 14 were involved in immune responses and inflammatory processes. Of these, the RT-PCR validation confirmed that Toll-like receptor 2 gene (TLR2) and myomesin 2 gene had a significantly higher expression in subjects with LAgP than in controls. RT-PCR also showed increased expression of TLR2 in subjects with RA. Comparison of subjects with GAgP to controls using microarray analysis identified only three upregulated genes. CONCLUSION: Several genes upregulated in subjects with LAgP were related to immune responses including TLR2 and myomesin 2.

U2 - 10.1902/jop.2008.070309

DO - 10.1902/jop.2008.070309

M3 - Journal article

C2 - 18315430

VL - 79

SP - 477

EP - 485

JO - Journal of Periodontology

JF - Journal of Periodontology

SN - 0022-3492

IS - 3

ER -