Abstract
Originalsprog | Engelsk |
---|---|
Tidsskrift | Journal of Cellular Physiology |
Vol/bind | 209 |
Udgave nummer | 3 |
Sider (fra-til) | 874-882 |
Antal sider | 8 |
ISSN | 0021-9541 |
DOI | |
Status | Udgivet - 2006 |
Bibliografisk note
(c) 2006 Wiley-Liss, Inc.Adgang til dokumentet
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Blood vessels and desmin control the positioning of nuclei in skeletal muscle fibers. / Ralston, E; Lu, Z; Biscocho, N; Soumaka, E; Mavroidis, M; Prats, C; Lømo, T; Capetanaki, Y; Ploug, T.
I: Journal of Cellular Physiology, Bind 209, Nr. 3, 2006, s. 874-882.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
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TY - JOUR
T1 - Blood vessels and desmin control the positioning of nuclei in skeletal muscle fibers
AU - Ralston, E
AU - Lu, Z
AU - Biscocho, N
AU - Soumaka, E
AU - Mavroidis, M
AU - Prats, C
AU - Lømo, T
AU - Capetanaki, Y
AU - Ploug, T
N1 - Keywords: Animals; Blood Vessels; Cell Nucleus; Desmin; Immunohistochemistry; Intermediate Filaments; Mice; Mice, Knockout; Muscle Denervation; Muscle Fibers, Skeletal; Muscle, Skeletal; Neuromuscular Junction; Rats
PY - 2006
Y1 - 2006
N2 - Skeletal muscle fibers contain hundreds to thousands of nuclei which lie immediately under the plasmalemma and are spaced out along the fiber, except for a small cluster of specialized nuclei at the neuromuscular junction. How the nuclei attain their positions along the fiber is not understood. Here we show that the nuclei are preferentially localized near blood vessels (BV), particularly in slow-twitch, oxidative fibers. Thus, in rat soleus muscle fibers, 81% of the nuclei appear next to BV. Lack of desmin markedly perturbs the distribution of nuclei along the fibers but does not prevent their close association with BV. Consistent with a role for desmin in the spacing of nuclei, we show that denervation affects the organization of desmin filaments as well as the distribution of nuclei. During chronic stimulation of denervated muscles, new BV form, along which muscle nuclei align themselves. We conclude that the positioning of nuclei along muscle fibers is plastic and that BV and desmin intermediate filaments each play a distinct role in the control of this positioning.
AB - Skeletal muscle fibers contain hundreds to thousands of nuclei which lie immediately under the plasmalemma and are spaced out along the fiber, except for a small cluster of specialized nuclei at the neuromuscular junction. How the nuclei attain their positions along the fiber is not understood. Here we show that the nuclei are preferentially localized near blood vessels (BV), particularly in slow-twitch, oxidative fibers. Thus, in rat soleus muscle fibers, 81% of the nuclei appear next to BV. Lack of desmin markedly perturbs the distribution of nuclei along the fibers but does not prevent their close association with BV. Consistent with a role for desmin in the spacing of nuclei, we show that denervation affects the organization of desmin filaments as well as the distribution of nuclei. During chronic stimulation of denervated muscles, new BV form, along which muscle nuclei align themselves. We conclude that the positioning of nuclei along muscle fibers is plastic and that BV and desmin intermediate filaments each play a distinct role in the control of this positioning.
U2 - 10.1002/jcp.20780
DO - 10.1002/jcp.20780
M3 - Journal article
C2 - 16972267
VL - 209
SP - 874
EP - 882
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
SN - 0021-9541
IS - 3
ER -