@article{4fe619b607cc49d0b652ba5d187dffef,
title = "BOD1L Is Required to Suppress Deleterious Resection of Stressed Replication Forks",
abstract = "Recognition and repair of damaged replication forks are essential to maintain genome stability and are coordinated by the combined action of the Fanconi anemia and homologous recombination pathways. These pathways are vital to protect stalled replication forks from uncontrolled nucleolytic activity, which otherwise causes irreparable genomic damage. Here, we identify BOD1L as a component of this fork protection pathway, which safeguards genome stability after replication stress. Loss of BOD1L confers exquisite cellular sensitivity to replication stress and uncontrolled resection of damaged replication forks, due to a failure to stabilize RAD51 at these forks. Blocking DNA2-dependent resection, or downregulation of the helicases BLM and FBH1, suppresses both catastrophic fork processing and the accumulation of chromosomal damage in BOD1L-deficient cells. Thus, our work implicates BOD1L as a critical regulator of genome integrity that restrains nucleolytic degradation of damaged replication forks.",
author = "Higgs, {Martin R.} and Reynolds, {John J.} and Alicja Winczura and Blackford, {Andrew N.} and Val{\'e}rie Borel and Miller, {Edward S.} and Anastasia Zlatanou and Jadwiga Nieminuszczy and Ryan, {Ellis L.} and Davies, {Nicholas J.} and Tatjana Stankovic and Boulton, {Simon J.} and Wojciech Niedzwiedz and Stewart, {Grant S.}",
note = "Funding Information: We are extremely grateful to Hongtao Yu, Luis Toledo, Michelle Debatisse, Beno{\^i}t Le Tallec, Ross Warrington, and Fumiko Esashi for valuable reagents. We thank Aga Gambus, Roger Grand, Eva Petermann, and Malcolm Taylor for invaluable discussions. M.R.H., E.S.M., and G.S.S. are funded by a Cancer Research UK (CR-UK) Senior Fellowship (C17183/A13030). J.J.R. is funded by the University of Birmingham and a Medical Research Council (MRC) project grant (MR/M009882/1). A.N.B., A.W., J.N., and W.N. are funded by Worldwide Cancer Research and MRC Senior Non-Clinical Fellowships awarded to W.N. A.Z. is funded by Worldwide Cancer Research (13-1012). E.L.R. is funded by an MRC Ph.D. studentship. N.J.D. and T.S. are funded by a Leukemia and Lymphoma Research program grant (11045). V.B. is funded by the European Research Council (ERC), and S.J.B. is supported by the Wellcome Trust, CR-UK, and the ERC (RecMitMei). Publisher Copyright: {\textcopyright} 2015 Elsevier Inc.",
year = "2015",
doi = "10.1016/j.molcel.2015.06.007",
language = "English",
volume = "59",
pages = "462--477",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",
}