TY - JOUR
T1 - Calcium ion activity in physiological salt solutions: influence of anions substituted for chloride
AU - Christoffersen, Gert Rene Juul
AU - Skibsted, Leif H
N1 - Key Word Index: Helix; nerve potentials; Calcium ions; methylsulphate; chloride
PY - 1975
Y1 - 1975
N2 - 1. Substitution by different anions for chloride in physiological salt solutions leads to binding between Ca2+ and the anion. Experiments designed to test effects of Cl- therefore often show mixed effects of changes in Cl- and Ca2+ activity. 2. This mixed effect is demonstrated in neurons of the snail, Helix pomatia: current-voltage characteristics and membrane potential are described during reduction of extracellular Cl- using different anions as substitutes. Methylsulphate is concluded to be the best substitute in this preparation.3. The association constants for the binding of Ca2+ to a number of anions is given.
AB - 1. Substitution by different anions for chloride in physiological salt solutions leads to binding between Ca2+ and the anion. Experiments designed to test effects of Cl- therefore often show mixed effects of changes in Cl- and Ca2+ activity. 2. This mixed effect is demonstrated in neurons of the snail, Helix pomatia: current-voltage characteristics and membrane potential are described during reduction of extracellular Cl- using different anions as substitutes. Methylsulphate is concluded to be the best substitute in this preparation.3. The association constants for the binding of Ca2+ to a number of anions is given.
U2 - 10.1016/S0300-9629(75)80094-6
DO - 10.1016/S0300-9629(75)80094-6
M3 - Journal article
SN - 0300-9629
VL - 52
SP - 317
EP - 322
JO - Comparative Biochemistry and Physiology - Part A: Comparative Physiology
JF - Comparative Biochemistry and Physiology - Part A: Comparative Physiology
IS - 2
ER -