Calmodulin interacts with PAC1 and VPAC2 receptors and regulates PACAP-induced FOS expression in human neuroblastoma cells

B. Falktoft; B. Georg; J. Fahrenkrug

Calmodulin interacts with PAC1 and VPAC2 receptors and regulates PACAP-induced FOS expression in human neuroblastoma cells

B. Falktoft, B. Georg, J. Fahrenkrug

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

6 Citationer (Scopus)

Abstract

The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) mediates its physiological functions through activation of PAC1, VPAC1 and VPAC2 receptors, and the ubiquitous Ca2+-sensor calmodulin has been implicated in PACAP-induced signaling. The immediate early response gene FOS is a well-known marker of neuronal activation, so we used a human neuroblastoma cell line NB-1 to explore the role of calmodulin in PACAP-induced FOS gene expression. We observed both short-term and prolonged altered PACAP-mediated activation of the FOS gene in the presence of the calmodulin-antagonist W-7. NB-I cells were shown to express PAC1 and VPAC2 receptors, and immunoprecipitation of both receptors displayed a co-association with calmodulin in the absence of Ca2+. Our findings indicate a novel mechanism of calmodulin in regulating PACAP signaling by possible interaction with the inactive state of PAC1 and VPAC2 receptors. (C) 2009 Elsevier Ltd. All rights reserved
Udgivelsesdato: 2009/4

Originalsprog	Engelsk
Tidsskrift	Neuropeptides
Vol/bind	43
Udgave nummer	2
Sider (fra-til)	53-61
Antal sider	8
ISSN	0143-4179
Status	Udgivet - 2009

Bibliografisk note

Times Cited: 1ArticleEnglishFalktoft, BBispebjerg Hosp, Dept Clin Biochem, Bispebjerg Bakke 23, DK-2400 Copenhagen, DenmarkCited References Count: 54437XRCHURCHILL LIVINGSTONEJOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLANDEDINBURGH

Citationsformater

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title = "Calmodulin interacts with PAC1 and VPAC2 receptors and regulates PACAP-induced FOS expression in human neuroblastoma cells",

abstract = "The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) mediates its physiological functions through activation of PAC1, VPAC1 and VPAC2 receptors, and the ubiquitous Ca2+-sensor calmodulin has been implicated in PACAP-induced signaling. The immediate early response gene FOS is a well-known marker of neuronal activation, so we used a human neuroblastoma cell line NB-1 to explore the role of calmodulin in PACAP-induced FOS gene expression. We observed both short-term and prolonged altered PACAP-mediated activation of the FOS gene in the presence of the calmodulin-antagonist W-7. NB-I cells were shown to express PAC1 and VPAC2 receptors, and immunoprecipitation of both receptors displayed a co-association with calmodulin in the absence of Ca2+. Our findings indicate a novel mechanism of calmodulin in regulating PACAP signaling by possible interaction with the inactive state of PAC1 and VPAC2 receptors. (C) 2009 Elsevier Ltd. All rights reserved Udgivelsesdato: 2009/4",

author = "B. Falktoft and B. Georg and J. Fahrenkrug",

note = "Times Cited: 1ArticleEnglishFalktoft, BBispebjerg Hosp, Dept Clin Biochem, Bispebjerg Bakke 23, DK-2400 Copenhagen, DenmarkCited References Count: 54437XRCHURCHILL LIVINGSTONEJOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLANDEDINBURGH",

year = "2009",

language = "English",

volume = "43",

pages = "53--61",

journal = "Neuropeptides",

issn = "0143-4179",

publisher = "Churchill Livingstone",

number = "2",

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TY - JOUR

T1 - Calmodulin interacts with PAC1 and VPAC2 receptors and regulates PACAP-induced FOS expression in human neuroblastoma cells

AU - Falktoft, B.

AU - Georg, B.

AU - Fahrenkrug, J.

N1 - Times Cited: 1ArticleEnglishFalktoft, BBispebjerg Hosp, Dept Clin Biochem, Bispebjerg Bakke 23, DK-2400 Copenhagen, DenmarkCited References Count: 54437XRCHURCHILL LIVINGSTONEJOURNAL PRODUCTION DEPT, ROBERT STEVENSON HOUSE, 1-3 BAXTERS PLACE, LEITH WALK, EDINBURGH EH1 3AF, MIDLOTHIAN, SCOTLANDEDINBURGH

PY - 2009

Y1 - 2009

N2 - The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) mediates its physiological functions through activation of PAC1, VPAC1 and VPAC2 receptors, and the ubiquitous Ca2+-sensor calmodulin has been implicated in PACAP-induced signaling. The immediate early response gene FOS is a well-known marker of neuronal activation, so we used a human neuroblastoma cell line NB-1 to explore the role of calmodulin in PACAP-induced FOS gene expression. We observed both short-term and prolonged altered PACAP-mediated activation of the FOS gene in the presence of the calmodulin-antagonist W-7. NB-I cells were shown to express PAC1 and VPAC2 receptors, and immunoprecipitation of both receptors displayed a co-association with calmodulin in the absence of Ca2+. Our findings indicate a novel mechanism of calmodulin in regulating PACAP signaling by possible interaction with the inactive state of PAC1 and VPAC2 receptors. (C) 2009 Elsevier Ltd. All rights reserved Udgivelsesdato: 2009/4

AB - The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) mediates its physiological functions through activation of PAC1, VPAC1 and VPAC2 receptors, and the ubiquitous Ca2+-sensor calmodulin has been implicated in PACAP-induced signaling. The immediate early response gene FOS is a well-known marker of neuronal activation, so we used a human neuroblastoma cell line NB-1 to explore the role of calmodulin in PACAP-induced FOS gene expression. We observed both short-term and prolonged altered PACAP-mediated activation of the FOS gene in the presence of the calmodulin-antagonist W-7. NB-I cells were shown to express PAC1 and VPAC2 receptors, and immunoprecipitation of both receptors displayed a co-association with calmodulin in the absence of Ca2+. Our findings indicate a novel mechanism of calmodulin in regulating PACAP signaling by possible interaction with the inactive state of PAC1 and VPAC2 receptors. (C) 2009 Elsevier Ltd. All rights reserved Udgivelsesdato: 2009/4

M3 - Journal article

SN - 0143-4179

VL - 43

SP - 53

EP - 61

JO - Neuropeptides

JF - Neuropeptides

IS - 2

ER -