Cell-penetrating peptides as carriers for transepithelial drug delivery in vitro

Bidragets oversatte titel: Celle penetrerende peptider som transepitheliel transportsystem for lægemidler in vitro

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningpeer review

Abstract

There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.
Bidragets oversatte titelCelle penetrerende peptider som transepitheliel transportsystem for lægemidler in vitro
OriginalsprogEngelsk
TitelCell-penetrating peptides : Methods and Protocols
RedaktørerÜlo Langel
Antal sider17
Vol/bind1324
ForlagHumana Press
Publikationsdato2015
Sider261-277
Kapitel17
ISBN (Trykt)978-1-4939-2805-7
DOI
StatusUdgivet - 2015

Emneord

  • Det Sundhedsvidenskabelige Fakultet
  • Biologics
  • Peptides
  • Drug delivery
  • Transepithel
  • in vitro
  • Cell-penetrating peptides
  • CPP
  • proteins
  • Methods
  • Protocols
  • Complexation
  • Microfluidics
  • Biophysics
  • Caco-2 cell model
  • Caco-2
  • Formulation
  • Drug formulation

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