Abstract
There is a growing interest in the use of cell-penetrating peptides (CPPs) as carriers for transepithelial drug delivery. This chapter gives an introduction to and discussion of the commonly used production and characterization methods for CPP–cargo samples including high-throughput cell viability screening. Moreover, we describe methods for permeation and cell viability assessment in the Caco-2 cell culture model with and without implementation of biosimilar mucus. Last, a method to assess metabolic degradation in vitro is described.
Bidragets oversatte titel | Celle penetrerende peptider som transepitheliel transportsystem for lægemidler in vitro |
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Originalsprog | Engelsk |
Titel | Cell-penetrating peptides : Methods and Protocols |
Redaktører | Ülo Langel |
Antal sider | 17 |
Vol/bind | 1324 |
Forlag | Humana Press |
Publikationsdato | 2015 |
Sider | 261-277 |
Kapitel | 17 |
ISBN (Trykt) | 978-1-4939-2805-7 |
DOI | |
Status | Udgivet - 2015 |
Emneord
- Det Sundhedsvidenskabelige Fakultet
- Biologics
- Peptides
- Drug delivery
- Transepithel
- in vitro
- Cell-penetrating peptides
- CPP
- proteins
- Methods
- Protocols
- Complexation
- Microfluidics
- Biophysics
- Caco-2 cell model
- Caco-2
- Formulation
- Drug formulation