Cellular and humoral immune responses in a population from the Baringo District, Kenya to Leishmania promastigote lipophosphoglycan

J A Kurtzhals; A S Hey; T G Theander; E Odera; C B Christensen; J I Githure; D K Koech; K U Schaefer; E Handman; A Kharazmi

Cellular and humoral immune responses in a population from the Baringo District, Kenya to Leishmania promastigote lipophosphoglycan

J A Kurtzhals, A S Hey, T G Theander, E Odera, C B Christensen, J I Githure, D K Koech, K U Schaefer, E Handman, A Kharazmi

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

30 Citationer (Scopus)

Abstract

Udgivelsesdato: 1992-Apr

Originalsprog	Engelsk
Tidsskrift	American Journal of Tropical Medicine and Hygiene
Vol/bind	46
Udgave nummer	4
Sider (fra-til)	480-8
Antal sider	8
ISSN	0002-9637
Status	Udgivet - 1992

Bibliografisk note

Keywords: Adolescent; Adult; Animals; Antibodies, Protozoan; Antigens, Protozoan; Child; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Glycosphingolipids; Humans; Immunity, Cellular; Interferon Type II; Kenya; Leishmania tropica; Leishmaniasis, Cutaneous; Leishmaniasis, Visceral; Lymphocyte Activation; Male; Tuberculin

Citationsformater

Cellular and humoral immune responses in a population from the Baringo District, Kenya to Leishmania promastigote lipophosphoglycan. / Kurtzhals, J A; Hey, A S; Theander, T G; Odera, E; Christensen, C B; Githure, J I; Koech, D K; Schaefer, K U; Handman, E; Kharazmi, A.

I: American Journal of Tropical Medicine and Hygiene, Bind 46, Nr. 4, 1992, s. 480-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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title = "Cellular and humoral immune responses in a population from the Baringo District, Kenya to Leishmania promastigote lipophosphoglycan",

abstract = "In a cross-sectional house-to-house study in a leishmaniasis-endemic area in Kenya, the cellular and humoral immune response to Leishmania lipophosphoglycan (LPG) was determined. Clinical data, peripheral blood mononuclear cells, and plasma were obtained from 50 individuals over the age of eight years. Lymphoproliferation and interferon-gamma (IFN-gamma) production by these cells were examined. It was shown that cells from all six individuals in the population with a history of kala-azar responded to LPG in the lymphocyte proliferation assay, and four of these six responded in the IFN-gamma assay. In contrast, cells from 12 of 44 individuals from the study area with no history of kala-azar and none of the five Danish control samples responded to LPG. Antibodies against LPG were detected by enzyme-linked immunosorbent assay in 45 of 50 plasma samples. Our findings clearly show that mononuclear cells from kala-azar patients cured of infection were able to respond to the LPG preparation. The finding of a specific cellular immune response to LPG in 12 of 44 individuals with no history of kala-azar is consistent with previous epidemiologic studies, in which it has been shown that a proportion of L. donovani infections run a subclinical course. The high frequency of individuals with antibodies against LPG might indicate that a majority of the population had been exposed to the parasite.(ABSTRACT TRUNCATED AT 250 WORDS)",

author = "Kurtzhals, {J A} and Hey, {A S} and Theander, {T G} and E Odera and Christensen, {C B} and Githure, {J I} and Koech, {D K} and Schaefer, {K U} and E Handman and A Kharazmi",

note = "Keywords: Adolescent; Adult; Animals; Antibodies, Protozoan; Antigens, Protozoan; Child; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Glycosphingolipids; Humans; Immunity, Cellular; Interferon Type II; Kenya; Leishmania tropica; Leishmaniasis, Cutaneous; Leishmaniasis, Visceral; Lymphocyte Activation; Male; Tuberculin",

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T1 - Cellular and humoral immune responses in a population from the Baringo District, Kenya to Leishmania promastigote lipophosphoglycan

AU - Kurtzhals, J A

AU - Hey, A S

AU - Theander, T G

AU - Odera, E

AU - Christensen, C B

AU - Githure, J I

AU - Koech, D K

AU - Schaefer, K U

AU - Handman, E

AU - Kharazmi, A

N1 - Keywords: Adolescent; Adult; Animals; Antibodies, Protozoan; Antigens, Protozoan; Child; Cross-Sectional Studies; Enzyme-Linked Immunosorbent Assay; Female; Glycosphingolipids; Humans; Immunity, Cellular; Interferon Type II; Kenya; Leishmania tropica; Leishmaniasis, Cutaneous; Leishmaniasis, Visceral; Lymphocyte Activation; Male; Tuberculin

PY - 1992

Y1 - 1992

N2 - In a cross-sectional house-to-house study in a leishmaniasis-endemic area in Kenya, the cellular and humoral immune response to Leishmania lipophosphoglycan (LPG) was determined. Clinical data, peripheral blood mononuclear cells, and plasma were obtained from 50 individuals over the age of eight years. Lymphoproliferation and interferon-gamma (IFN-gamma) production by these cells were examined. It was shown that cells from all six individuals in the population with a history of kala-azar responded to LPG in the lymphocyte proliferation assay, and four of these six responded in the IFN-gamma assay. In contrast, cells from 12 of 44 individuals from the study area with no history of kala-azar and none of the five Danish control samples responded to LPG. Antibodies against LPG were detected by enzyme-linked immunosorbent assay in 45 of 50 plasma samples. Our findings clearly show that mononuclear cells from kala-azar patients cured of infection were able to respond to the LPG preparation. The finding of a specific cellular immune response to LPG in 12 of 44 individuals with no history of kala-azar is consistent with previous epidemiologic studies, in which it has been shown that a proportion of L. donovani infections run a subclinical course. The high frequency of individuals with antibodies against LPG might indicate that a majority of the population had been exposed to the parasite.(ABSTRACT TRUNCATED AT 250 WORDS)

AB - In a cross-sectional house-to-house study in a leishmaniasis-endemic area in Kenya, the cellular and humoral immune response to Leishmania lipophosphoglycan (LPG) was determined. Clinical data, peripheral blood mononuclear cells, and plasma were obtained from 50 individuals over the age of eight years. Lymphoproliferation and interferon-gamma (IFN-gamma) production by these cells were examined. It was shown that cells from all six individuals in the population with a history of kala-azar responded to LPG in the lymphocyte proliferation assay, and four of these six responded in the IFN-gamma assay. In contrast, cells from 12 of 44 individuals from the study area with no history of kala-azar and none of the five Danish control samples responded to LPG. Antibodies against LPG were detected by enzyme-linked immunosorbent assay in 45 of 50 plasma samples. Our findings clearly show that mononuclear cells from kala-azar patients cured of infection were able to respond to the LPG preparation. The finding of a specific cellular immune response to LPG in 12 of 44 individuals with no history of kala-azar is consistent with previous epidemiologic studies, in which it has been shown that a proportion of L. donovani infections run a subclinical course. The high frequency of individuals with antibodies against LPG might indicate that a majority of the population had been exposed to the parasite.(ABSTRACT TRUNCATED AT 250 WORDS)

M3 - Journal article

C2 - 1575296

VL - 46

SP - 480

EP - 488

JO - Journal. National Malaria Society

JF - Journal. National Malaria Society

SN - 0002-9637

IS - 4

ER -