Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment

Lotte Jakobsen; Dorthe Sandvang; Lars H Hansen; Line Bagger-Skjøt; Henrik Westh; Claus Jørgensen; Dennis S Hansen; Bodil M Pedersen; Dominique L Monnet; Niels Frimodt-Møller; Søren J Sørensen; Anette M Hammerum

doi:10.1016/j.envint.2007.07.011

Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment

Lotte Jakobsen, Dorthe Sandvang, Lars H Hansen, Line Bagger-Skjøt, Henrik Westh, Claus Jørgensen, Dennis S Hansen, Bodil M Pedersen, Dominique L Monnet, Niels Frimodt-Møller, Søren J Sørensen, Anette M Hammerum

Microbiology

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

64 Citationer (Scopus)

Abstract

Udgivelsesdato: 2008-Jan

Originalsprog	Engelsk
Tidsskrift	Environment International
Vol/bind	34
Udgave nummer	1
Sider (fra-til)	108-15
Antal sider	7
ISSN	0160-4120
DOI	https://doi.org/10.1016/j.envint.2007.07.011
Status	Udgivet - 2008

Bibliografisk note

Keywords: Anti-Bacterial Agents; Cluster Analysis; Conjugation, Genetic; DNA Fingerprinting; DNA, Bacterial; Denmark; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-Field; Escherichia coli; Gene Transfer, Horizontal; Genes, Bacterial; Genotype; Gentamicins; Hospitals, University; Humans; Microbial Sensitivity Tests; Virulence Factors; Waste Disposal, Fluid; Water Microbiology

Adgang til dokumentet

10.1016/j.envint.2007.07.011

Citationsformater

Jakobsen, L., Sandvang, D., Hansen, L. H., Bagger-Skjøt, L., Westh, H., Jørgensen, C., Hansen, D. S., Pedersen, B. M., Monnet, D. L., Frimodt-Møller, N., Sørensen, S. J., & Hammerum, A. M. (2008). Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment. Environment International, 34(1), 108-15. https://doi.org/10.1016/j.envint.2007.07.011

Jakobsen, L, Sandvang, D, Hansen, LH, Bagger-Skjøt, L, Westh, H, Jørgensen, C, Hansen, DS, Pedersen, BM, Monnet, DL, Frimodt-Møller, N, Sørensen, SJ & Hammerum, AM 2008, 'Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment', Environment International, bind 34, nr. 1, s. 108-15. https://doi.org/10.1016/j.envint.2007.07.011

@article{b00265d0dd8c11ddb5fc000ea68e967b,

title = "Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment",

abstract = "The aim of the study was to investigate the potential spread of gentamicin resistant (GEN(R)) Escherichia coli isolates or GEN(R) determinants from a Danish university hospital to the waste water environment. Waste water samples were collected monthly from the outlets of the hospital bed wards and the inlet of the related waste water treatment plant (WWTP) from October 2002 to August 2003. Waste water samples were also collected monthly from a residential area in the same period to be able to compare the prevalence of GEN(R)E. coli isolates from hospital related and residential waste water. The waste water isolates were compared to GEN(R)E. coli isolates obtained consecutively from September 2002 to September 2003 from patients mainly with urinary tract infections at the hospital with respect to Pulsed Field Gel Electrophoresis (PFGE) profiles. All isolates were investigated for GEN(R) mechanisms (aac(3)-II, aac(3)-IV, ant(2'')-I, armA), phenotypic resistance pattern, and virulence genes (hlyA, chuA, sfaS, fogG, malX, traT, iutA, fyuA, iroN, cnf1) to investigate if the hospital and waste water could be reservoirs of antimicrobial resistance and virulence. The ability for GEN(R) determinants to transfer horizontally was investigated by mating experiments. A total of 38, 15, 21, and two GEN(R)E. coli were isolated from patients, the hospital outlets, the inlet of the WWTP, and the residential area, respectively. GEN(R)E. coli were more prevalent in waste water from the hospital and the WWTP than in waste water from the residential area. PFGE profiling revealed no spread of specific patient isolates to the waste water. The aac(3)-II gene was detected both in patient and waste water isolates. Furthermore horizontal transfer of the aac(3)-II gene of patient origin to a recipient was shown in vitro, indicating a potential spread of the gene from patient isolates to waste water isolates. Regardless of origin, most isolates exhibited multi-resistance and contained several virulence genes. In conclusion, our study showed a possible spread of aac(3)-II from the hospital to the waste water. Most of the GEN(R)E. coli isolates from both patients and waste water had a multi-resistant phenotype and contained virulence genes and should therefore be considered reservoirs of antimicrobial resistance and virulence genes.",

author = "Lotte Jakobsen and Dorthe Sandvang and Hansen, {Lars H} and Line Bagger-Skj{\o}t and Henrik Westh and Claus J{\o}rgensen and Hansen, {Dennis S} and Pedersen, {Bodil M} and Monnet, {Dominique L} and Niels Frimodt-M{\o}ller and S{\o}rensen, {S{\o}ren J} and Hammerum, {Anette M}",

note = "Keywords: Anti-Bacterial Agents; Cluster Analysis; Conjugation, Genetic; DNA Fingerprinting; DNA, Bacterial; Denmark; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-Field; Escherichia coli; Gene Transfer, Horizontal; Genes, Bacterial; Genotype; Gentamicins; Hospitals, University; Humans; Microbial Sensitivity Tests; Virulence Factors; Waste Disposal, Fluid; Water Microbiology",

year = "2008",

doi = "10.1016/j.envint.2007.07.011",

language = "English",

volume = "34",

pages = "108--15",

journal = "Environment International",

issn = "0160-4120",

publisher = "Pergamon Press",

number = "1",

}

TY - JOUR

T1 - Characterisation, dissemination and persistence of gentamicin resistant Escherichia coli from a Danish university hospital to the waste water environment

AU - Jakobsen, Lotte

AU - Sandvang, Dorthe

AU - Hansen, Lars H

AU - Bagger-Skjøt, Line

AU - Westh, Henrik

AU - Jørgensen, Claus

AU - Hansen, Dennis S

AU - Pedersen, Bodil M

AU - Monnet, Dominique L

AU - Frimodt-Møller, Niels

AU - Sørensen, Søren J

AU - Hammerum, Anette M

N1 - Keywords: Anti-Bacterial Agents; Cluster Analysis; Conjugation, Genetic; DNA Fingerprinting; DNA, Bacterial; Denmark; Drug Resistance, Bacterial; Drug Resistance, Multiple, Bacterial; Electrophoresis, Gel, Pulsed-Field; Escherichia coli; Gene Transfer, Horizontal; Genes, Bacterial; Genotype; Gentamicins; Hospitals, University; Humans; Microbial Sensitivity Tests; Virulence Factors; Waste Disposal, Fluid; Water Microbiology

PY - 2008

Y1 - 2008

N2 - The aim of the study was to investigate the potential spread of gentamicin resistant (GEN(R)) Escherichia coli isolates or GEN(R) determinants from a Danish university hospital to the waste water environment. Waste water samples were collected monthly from the outlets of the hospital bed wards and the inlet of the related waste water treatment plant (WWTP) from October 2002 to August 2003. Waste water samples were also collected monthly from a residential area in the same period to be able to compare the prevalence of GEN(R)E. coli isolates from hospital related and residential waste water. The waste water isolates were compared to GEN(R)E. coli isolates obtained consecutively from September 2002 to September 2003 from patients mainly with urinary tract infections at the hospital with respect to Pulsed Field Gel Electrophoresis (PFGE) profiles. All isolates were investigated for GEN(R) mechanisms (aac(3)-II, aac(3)-IV, ant(2'')-I, armA), phenotypic resistance pattern, and virulence genes (hlyA, chuA, sfaS, fogG, malX, traT, iutA, fyuA, iroN, cnf1) to investigate if the hospital and waste water could be reservoirs of antimicrobial resistance and virulence. The ability for GEN(R) determinants to transfer horizontally was investigated by mating experiments. A total of 38, 15, 21, and two GEN(R)E. coli were isolated from patients, the hospital outlets, the inlet of the WWTP, and the residential area, respectively. GEN(R)E. coli were more prevalent in waste water from the hospital and the WWTP than in waste water from the residential area. PFGE profiling revealed no spread of specific patient isolates to the waste water. The aac(3)-II gene was detected both in patient and waste water isolates. Furthermore horizontal transfer of the aac(3)-II gene of patient origin to a recipient was shown in vitro, indicating a potential spread of the gene from patient isolates to waste water isolates. Regardless of origin, most isolates exhibited multi-resistance and contained several virulence genes. In conclusion, our study showed a possible spread of aac(3)-II from the hospital to the waste water. Most of the GEN(R)E. coli isolates from both patients and waste water had a multi-resistant phenotype and contained virulence genes and should therefore be considered reservoirs of antimicrobial resistance and virulence genes.

AB - The aim of the study was to investigate the potential spread of gentamicin resistant (GEN(R)) Escherichia coli isolates or GEN(R) determinants from a Danish university hospital to the waste water environment. Waste water samples were collected monthly from the outlets of the hospital bed wards and the inlet of the related waste water treatment plant (WWTP) from October 2002 to August 2003. Waste water samples were also collected monthly from a residential area in the same period to be able to compare the prevalence of GEN(R)E. coli isolates from hospital related and residential waste water. The waste water isolates were compared to GEN(R)E. coli isolates obtained consecutively from September 2002 to September 2003 from patients mainly with urinary tract infections at the hospital with respect to Pulsed Field Gel Electrophoresis (PFGE) profiles. All isolates were investigated for GEN(R) mechanisms (aac(3)-II, aac(3)-IV, ant(2'')-I, armA), phenotypic resistance pattern, and virulence genes (hlyA, chuA, sfaS, fogG, malX, traT, iutA, fyuA, iroN, cnf1) to investigate if the hospital and waste water could be reservoirs of antimicrobial resistance and virulence. The ability for GEN(R) determinants to transfer horizontally was investigated by mating experiments. A total of 38, 15, 21, and two GEN(R)E. coli were isolated from patients, the hospital outlets, the inlet of the WWTP, and the residential area, respectively. GEN(R)E. coli were more prevalent in waste water from the hospital and the WWTP than in waste water from the residential area. PFGE profiling revealed no spread of specific patient isolates to the waste water. The aac(3)-II gene was detected both in patient and waste water isolates. Furthermore horizontal transfer of the aac(3)-II gene of patient origin to a recipient was shown in vitro, indicating a potential spread of the gene from patient isolates to waste water isolates. Regardless of origin, most isolates exhibited multi-resistance and contained several virulence genes. In conclusion, our study showed a possible spread of aac(3)-II from the hospital to the waste water. Most of the GEN(R)E. coli isolates from both patients and waste water had a multi-resistant phenotype and contained virulence genes and should therefore be considered reservoirs of antimicrobial resistance and virulence genes.

U2 - 10.1016/j.envint.2007.07.011

DO - 10.1016/j.envint.2007.07.011

M3 - Journal article

C2 - 17804070

SN - 0160-4120

VL - 34

SP - 108

EP - 115

JO - Environment International

JF - Environment International

IS - 1

ER -