Characterization and Abundance of Plasmid-Dependent Alphatectivirus Bacteriophages

Boris Parra, Veronika T. Lutz, Lone Brøndsted, Javiera L. Carmona, Alejandro Palomo, Joseph Nesme, Vuong Van Hung Le, Barth F. Smets, Arnaud Dechesne*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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Abstract

Antimicrobial resistance (AMR) is a major public health threat, exacerbated by the ability of bacteria to rapidly disseminate antimicrobial resistance genes (ARG). Since conjugative plasmids of the incompatibility group P (IncP) are ubiquitous mobile genetic elements that often carry ARG and are broad-host-range, they are important targets to prevent the dissemination of AMR. Plasmid-dependent phages infect plasmid-carrying bacteria by recognizing components of the conjugative secretion system as receptors. We sought to isolate plasmid-dependent phages from wastewater using an avirulent strain of Salmonella enterica carrying the conjugative IncP plasmid pKJK5. Irrespective of the site, we only obtained bacteriophages belonging to the genus Alphatectivirus. Eleven isolates were sequenced, their genomes analyzed, and their host range established using S. enterica, Escherichia coli, and Pseudomonas putida carrying diverse conjugative plasmids. We confirmed that Alphatectivirus are abundant in domestic and hospital wastewater using culture-dependent and culture-independent approaches. However, these results are not consistent with their low or undetectable occurrence in metagenomes. Therefore, overall, our results emphasize the importance of performing phage isolation to uncover diversity, especially considering the potential of plasmid-dependent phages to reduce the spread of ARG carried by conjugative plasmids, and to help combat the AMR crisis.
OriginalsprogEngelsk
Artikelnummer85
TidsskriftMicrobial Ecology
Vol/bind87
Antal sider11
ISSN0095-3628
DOI
StatusUdgivet - 2024

Bibliografisk note

Funding Information:
Open access funding provided by Technical University of Denmark. This project has received funding from the European Union\u2019s Horizon 2020 research and innovation program under the Marie Sklodowska-Curie grant agreement No 101026675, the research grant \u201CP-PhanFARE (23046)\u201D from VILLUM FONDEN and the grant \u201CVRID Postdoctorado from Vicerrector\u00EDa de Investigaci\u00F3n y Desarrollo, Universidad de Concepci\u00F3n \u201Cproject CO 002200000279\u201D.

Publisher Copyright:
© The Author(s) 2024.

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