Characterization and transfer studies of macrolide resistance genes in Streptococcus pneumoniae from Denmark

Karen L Nielsen; Anette M Hammerum; Lotte M Lambertsen; Camilla H Lester; Magnus Arpi; Jenny D Knudsen; Marc Stegger; Tim Tolker-Nielsen; Niels Frimodt-Møller

doi:10.3109/00365541003754451

Characterization and transfer studies of macrolide resistance genes in Streptococcus pneumoniae from Denmark

Karen L Nielsen, Anette M Hammerum, Lotte M Lambertsen, Camilla H Lester, Magnus Arpi, Jenny D Knudsen, Marc Stegger, Tim Tolker-Nielsen, Niels Frimodt-Møller

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

5 Citationer (Scopus)

Abstract

Udgivelsesdato: 2010-Aug

Originalsprog	Engelsk
Tidsskrift	Scandinavian Journal of Infectious Diseases
Vol/bind	42
Udgave nummer	8
Sider (fra-til)	586-93
Antal sider	7
ISSN	0036-5548
DOI	https://doi.org/10.3109/00365541003754451
Status	Udgivet - 2010

Bibliografisk note

Keywords: Anti-Bacterial Agents; Conjugation, Genetic; DNA, Bacterial; Denmark; Drug Resistance, Bacterial; Gene Transfer, Horizontal; Humans; Macrolides; Microbial Sensitivity Tests; Pneumococcal Infections; Point Mutation; Sequence Analysis, DNA; Streptococcus pneumoniae; Transformation, Bacterial

Adgang til dokumentet

10.3109/00365541003754451

Citationsformater

@article{3aba4c00e72811dfb6d2000ea68e967b,

title = "Characterization and transfer studies of macrolide resistance genes in Streptococcus pneumoniae from Denmark",

abstract = "Over the last decade, erythromycin resistance has been increasing in frequency in Streptococcus pneumoniae in Denmark. In the present study, 49 non-related erythromycin-resistant S. pneumoniae isolates from invasive sites and 20 isolates from non-invasive sites were collected; antimicrobial susceptibility was tested, and they were genotyped and serotyped. Gene transfer was studied for selected isolates. The frequency of erm(B) was significantly higher in non-invasive isolates compared to invasive isolates (p = 0.001). For the first time, mef(I) was detected in 1 isolate in Denmark. All tested mef(E) isolates had an identical mef(E) sequence, apart from 1 gene with a point mutation, and mef(E) was correlated to 7 different sero-types. The tested erm(B) sequences were 99.3% similar with 5 point mutations at different positions distributed among different serotypes, which did not cause a detectable influence on the protein. Transformation was detectable in 5 out of 13 isolates and transfer of erm(B), mef(I) and mef(E) was detected. To our knowledge, this is the first time mef(I) has been proved transformable. Gene transfer by conjugation was not detectable. Erythromycin resistance in pneumococcal isolates is likely to be caused primarily by horizontal spread of mef(E) and erm(B), as well as clonal spread of a serotype 14 strain carrying mef(A) primarily detected in invasive isolates.",

author = "Nielsen, {Karen L} and Hammerum, {Anette M} and Lambertsen, {Lotte M} and Lester, {Camilla H} and Magnus Arpi and Knudsen, {Jenny D} and Marc Stegger and Tim Tolker-Nielsen and Niels Frimodt-M{\o}ller",

note = "Keywords: Anti-Bacterial Agents; Conjugation, Genetic; DNA, Bacterial; Denmark; Drug Resistance, Bacterial; Gene Transfer, Horizontal; Humans; Macrolides; Microbial Sensitivity Tests; Pneumococcal Infections; Point Mutation; Sequence Analysis, DNA; Streptococcus pneumoniae; Transformation, Bacterial",

year = "2010",

doi = "10.3109/00365541003754451",

language = "English",

volume = "42",

pages = "586--93",

journal = "Scandinavian Journal of Infectious Diseases",

issn = "0036-5548",

publisher = "Taylor & Francis",

number = "8",

}

TY - JOUR

T1 - Characterization and transfer studies of macrolide resistance genes in Streptococcus pneumoniae from Denmark

AU - Nielsen, Karen L

AU - Hammerum, Anette M

AU - Lambertsen, Lotte M

AU - Lester, Camilla H

AU - Arpi, Magnus

AU - Knudsen, Jenny D

AU - Stegger, Marc

AU - Tolker-Nielsen, Tim

AU - Frimodt-Møller, Niels

N1 - Keywords: Anti-Bacterial Agents; Conjugation, Genetic; DNA, Bacterial; Denmark; Drug Resistance, Bacterial; Gene Transfer, Horizontal; Humans; Macrolides; Microbial Sensitivity Tests; Pneumococcal Infections; Point Mutation; Sequence Analysis, DNA; Streptococcus pneumoniae; Transformation, Bacterial

PY - 2010

Y1 - 2010

N2 - Over the last decade, erythromycin resistance has been increasing in frequency in Streptococcus pneumoniae in Denmark. In the present study, 49 non-related erythromycin-resistant S. pneumoniae isolates from invasive sites and 20 isolates from non-invasive sites were collected; antimicrobial susceptibility was tested, and they were genotyped and serotyped. Gene transfer was studied for selected isolates. The frequency of erm(B) was significantly higher in non-invasive isolates compared to invasive isolates (p = 0.001). For the first time, mef(I) was detected in 1 isolate in Denmark. All tested mef(E) isolates had an identical mef(E) sequence, apart from 1 gene with a point mutation, and mef(E) was correlated to 7 different sero-types. The tested erm(B) sequences were 99.3% similar with 5 point mutations at different positions distributed among different serotypes, which did not cause a detectable influence on the protein. Transformation was detectable in 5 out of 13 isolates and transfer of erm(B), mef(I) and mef(E) was detected. To our knowledge, this is the first time mef(I) has been proved transformable. Gene transfer by conjugation was not detectable. Erythromycin resistance in pneumococcal isolates is likely to be caused primarily by horizontal spread of mef(E) and erm(B), as well as clonal spread of a serotype 14 strain carrying mef(A) primarily detected in invasive isolates.

AB - Over the last decade, erythromycin resistance has been increasing in frequency in Streptococcus pneumoniae in Denmark. In the present study, 49 non-related erythromycin-resistant S. pneumoniae isolates from invasive sites and 20 isolates from non-invasive sites were collected; antimicrobial susceptibility was tested, and they were genotyped and serotyped. Gene transfer was studied for selected isolates. The frequency of erm(B) was significantly higher in non-invasive isolates compared to invasive isolates (p = 0.001). For the first time, mef(I) was detected in 1 isolate in Denmark. All tested mef(E) isolates had an identical mef(E) sequence, apart from 1 gene with a point mutation, and mef(E) was correlated to 7 different sero-types. The tested erm(B) sequences were 99.3% similar with 5 point mutations at different positions distributed among different serotypes, which did not cause a detectable influence on the protein. Transformation was detectable in 5 out of 13 isolates and transfer of erm(B), mef(I) and mef(E) was detected. To our knowledge, this is the first time mef(I) has been proved transformable. Gene transfer by conjugation was not detectable. Erythromycin resistance in pneumococcal isolates is likely to be caused primarily by horizontal spread of mef(E) and erm(B), as well as clonal spread of a serotype 14 strain carrying mef(A) primarily detected in invasive isolates.

U2 - 10.3109/00365541003754451

DO - 10.3109/00365541003754451

M3 - Journal article

C2 - 20429715

SN - 0036-5548

VL - 42

SP - 586

EP - 593

JO - Scandinavian Journal of Infectious Diseases

JF - Scandinavian Journal of Infectious Diseases

IS - 8

ER -