Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers

Cyril Jean-Marie Martel; Bent Aasted

doi:10.1016/j.vetimm.2009.05.011

Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers

Cyril Jean-Marie Martel, Bent Aasted

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

22 Citationer (Scopus)

Abstract

Ferret IgG and IgM were purified from normal serum, while ferret IgA was purified from bile. The estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa, respectively. For immunological (ELISA) quantification of ferret immunoglobulins, we identified and characterized polyclonal antibodies towards ferret IgG, IgM and IgA. We also identified 22 monoclonal antibodies (mAbs) raised mostly against human CD markers which cross-reacted with ferret leukocytes. These antibodies were originally specific against human CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and mink CD3. Finally, we identified 4 cross-reacting mAbs with specificities against ferret interferon-gamma, TNF-alpha, interleukin-4 and interleukin-8.

Originalsprog	Engelsk
Tidsskrift	Veterinary Immunology and Immunopathology
Vol/bind	132
Udgave nummer	2-4
Sider (fra-til)	109-115
Antal sider	7
ISSN	0165-2427
DOI	https://doi.org/10.1016/j.vetimm.2009.05.011
Status	Udgivet - 2009

Adgang til dokumentet

10.1016/j.vetimm.2009.05.011

Citationsformater

@article{f032a98090a111de8bc9000ea68e967b,

title = "Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers",

abstract = "Ferret IgG and IgM were purified from normal serum, while ferret IgA was purified from bile. The estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa, respectively. For immunological (ELISA) quantification of ferret immunoglobulins, we identified and characterized polyclonal antibodies towards ferret IgG, IgM and IgA. We also identified 22 monoclonal antibodies (mAbs) raised mostly against human CD markers which cross-reacted with ferret leukocytes. These antibodies were originally specific against human CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and mink CD3. Finally, we identified 4 cross-reacting mAbs with specificities against ferret interferon-gamma, TNF-alpha, interleukin-4 and interleukin-8.",

author = "Martel, {Cyril Jean-Marie} and Bent Aasted",

year = "2009",

doi = "10.1016/j.vetimm.2009.05.011",

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TY - JOUR

T1 - Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers

AU - Martel, Cyril Jean-Marie

AU - Aasted, Bent

PY - 2009

Y1 - 2009

N2 - Ferret IgG and IgM were purified from normal serum, while ferret IgA was purified from bile. The estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa, respectively. For immunological (ELISA) quantification of ferret immunoglobulins, we identified and characterized polyclonal antibodies towards ferret IgG, IgM and IgA. We also identified 22 monoclonal antibodies (mAbs) raised mostly against human CD markers which cross-reacted with ferret leukocytes. These antibodies were originally specific against human CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and mink CD3. Finally, we identified 4 cross-reacting mAbs with specificities against ferret interferon-gamma, TNF-alpha, interleukin-4 and interleukin-8.

AB - Ferret IgG and IgM were purified from normal serum, while ferret IgA was purified from bile. The estimated molecular weights of the immunoglobulin gamma, alpha and mu heavy chains were found to be 54 kDa, 69 kDa and 83 kDa, respectively. For immunological (ELISA) quantification of ferret immunoglobulins, we identified and characterized polyclonal antibodies towards ferret IgG, IgM and IgA. We also identified 22 monoclonal antibodies (mAbs) raised mostly against human CD markers which cross-reacted with ferret leukocytes. These antibodies were originally specific against human CD8, CD9, CD14, CD18, CD25, CD29, CD32, CD44, CD61, CD71, CD79b, CD88, CD104, CD172a and mink CD3. Finally, we identified 4 cross-reacting mAbs with specificities against ferret interferon-gamma, TNF-alpha, interleukin-4 and interleukin-8.

U2 - 10.1016/j.vetimm.2009.05.011

DO - 10.1016/j.vetimm.2009.05.011

M3 - Journal article

C2 - 19505731

SN - 0165-2427

VL - 132

SP - 109

EP - 115

JO - Veterinary Immunology and Immunopathology

JF - Veterinary Immunology and Immunopathology

IS - 2-4

ER -