TY - JOUR
T1 - Characterization of Mast Cells from Healthy and Varicose Human Saphenous Vein
AU - Callesen, Katrine T
AU - Mogren, Sofia
AU - Berlin, Frida
AU - Andersson, Cecilia
AU - Schmidt, Susanne
AU - Klitfod, Lotte
AU - Esteban, Vanesa
AU - Poulsen, Lars K
AU - Jensen, Bettina M
PY - 2022
Y1 - 2022
N2 - Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MC
T and MC
TC phenotypes (vSV: 55% MC
T, hSV: 64% MC
T). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcεRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.
AB - Mast cells (MCs) are distributed in tissues throughout the body and are highly involved in many physiological and pathophysiological processes. The potential and involvement of different MC phenotypes are still not well understood. MCs are present in blood vessel walls, but their specific phenotypic features are unknown. We aimed at characterizing MCs from human saphenous veins for localization, mediator content, and receptor expression. This was done in MCs from both healthy and varicose human saphenous veins (hSV and vSV, respectively). For both vSV and hSV, we found that vein MCs are mainly present in the tunica adventitia (99% MCs in adventitia) and that the population consists of both MC
T and MC
TC phenotypes (vSV: 55% MC
T, hSV: 64% MC
T). The vein MCs contained high levels of histamine (vSV: 27 pg/MC, hSV: 55 pg/MC) and tryptase (vSV: 98 pg/MC, hSV: 111 pg/MC), indicating a strong potential for regulatory effects on blood vessels. The receptor expression of FcεRI, MRGPRX2, PTAFR, C3aR, and C5aR was found, even though the percentage of positive cells differed between vSV and hSV MCs. We conclude that vein MCs from the blood vessel wall have a high potential to affect the tissue around them.
U2 - 10.3390/biomedicines10051062
DO - 10.3390/biomedicines10051062
M3 - Journal article
C2 - 35625799
VL - 10
JO - Biomedicines
JF - Biomedicines
SN - 2227-9059
IS - 5
ER -