Circulating chromatin-anti-chromatin antibody complexes bind with high affinity to dermo-epidermal structures in murine and human lupus nephritis

S Fismen; A Hedberg; K A Fenton; S Jacobsen; E Krarup; A L Kamper; O P Rekvig; E S Mortensen; S Fismen; A Hedberg; K A Fenton; S Jacobsen; E Krarup; A L Kamper; O P Rekvig; E S Mortensen

doi:10.1177/0961203308100512

Circulating chromatin-anti-chromatin antibody complexes bind with high affinity to dermo-epidermal structures in murine and human lupus nephritis

S Fismen, A Hedberg, K A Fenton, S Jacobsen, E Krarup, A L Kamper, O P Rekvig, E S Mortensen, S Fismen, A Hedberg, K A Fenton, S Jacobsen, E Krarup, A L Kamper, O P Rekvig, E S Mortensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

37 Citationer (Scopus)

Abstract

Udgivelsesdato: 2009-Jun

Originalsprog	Engelsk
Tidsskrift	Lupus
Vol/bind	18
Udgave nummer	7
Sider (fra-til)	597-607
Antal sider	11
ISSN	0961-2033
DOI	https://doi.org/10.1177/0961203308100512 https://doi.org/10.1177/0961203308100512
Status	Udgivet - 1 jun. 2009

Bibliografisk note

Keywords: Animals; Antibodies, Anti-Idiotypic; Antigen-Antibody Complex; Biopsy; Capillaries; Cell Adhesion Molecules; Chromatin; Collagen Type I; Collagen Type IV; Disease Models, Animal; Female; Histones; Humans; Immunoglobulin G; Kidney Glomerulus; Lupus Nephritis; Mice; Mice, Inbred MRL lpr; Mice, Inbred NZB; Skin; Transcription Factors

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Citationsformater

Fismen, S., Hedberg, A., Fenton, K. A., Jacobsen, S., Krarup, E., Kamper, A. L., Rekvig, O. P., Mortensen, E. S., Fismen, S., Hedberg, A., Fenton, K. A., Jacobsen, S., Krarup, E., Kamper, A. L., Rekvig, O. P., & Mortensen, E. S. (2009). Circulating chromatin-anti-chromatin antibody complexes bind with high affinity to dermo-epidermal structures in murine and human lupus nephritis. Lupus, 18(7), 597-607. https://doi.org/10.1177/0961203308100512, https://doi.org/10.1177/0961203308100512

Fismen, S, Hedberg, A, Fenton, KA, Jacobsen, S, Krarup, E, Kamper, AL, Rekvig, OP, Mortensen, ES, Fismen, S, Hedberg, A, Fenton, KA, Jacobsen, S, Krarup, E, Kamper, AL, Rekvig, OP & Mortensen, ES 2009, 'Circulating chromatin-anti-chromatin antibody complexes bind with high affinity to dermo-epidermal structures in murine and human lupus nephritis', Lupus, bind 18, nr. 7, s. 597-607. https://doi.org/10.1177/0961203308100512, https://doi.org/10.1177/0961203308100512

@article{013eecc06a3b11df928f000ea68e967b,

title = "Circulating chromatin-anti-chromatin antibody complexes bind with high affinity to dermo-epidermal structures in murine and human lupus nephritis",

abstract = "Murine and human lupus nephritis are characterized by glomerular deposits of electron-dense structures (EDS). Dominant components of EDS are chromatin fragments and IgG antibodies. Whether glomerular EDS predispose for similar deposits in skin is unknown. We analysed (i) whether dermo-epidermal immune complex deposits have similar molecular composition as glomerular deposits, (ii) whether chromatin fragments bind dermo-epidermal structures, and (iii) whether deposits in nephritic glomeruli predispose for accumulation of similar deposits in skin. Paired skin and kidney biopsies from nephritic (NZBxNZW)F1 and MRL-lpr/lpr mice and from five patients with lupus nephritis were analysed by immunofluorescence, immune electron microscopy (IEM) and co-localization TUNEL IEM. Affinity of chromatin fragments for membrane structures was determined by surface plasmon resonance. Results demonstrated (i) presence of EDS containing chromatin fragments and IgG in both organs in nephritic patients, (ii) chromatin fragments possessed high affinity for dermo-epidermal laminins and collagens, (iii) glomerular immune complex deposits did not predict similar interstitial deposits in skin, although such complexes were present in capillary lumina in glomeruli and skin of all nephritic individuals. Thus, chromatin-IgG complexes accounting for lupus nephritis seem to reach skin through circulation, but other undetermined factors are required for these complexes to deposit within skin membranes.",

author = "S Fismen and A Hedberg and Fenton, {K A} and S Jacobsen and E Krarup and Kamper, {A L} and Rekvig, {O P} and Mortensen, {E S} and S Fismen and A Hedberg and Fenton, {K A} and S Jacobsen and E Krarup and Kamper, {A L} and Rekvig, {O P} and Mortensen, {E S}",

note = "Keywords: Animals; Antibodies, Anti-Idiotypic; Antigen-Antibody Complex; Biopsy; Capillaries; Cell Adhesion Molecules; Chromatin; Collagen Type I; Collagen Type IV; Disease Models, Animal; Female; Histones; Humans; Immunoglobulin G; Kidney Glomerulus; Lupus Nephritis; Mice; Mice, Inbred MRL lpr; Mice, Inbred NZB; Skin; Transcription Factors",

year = "2009",

month = jun,

day = "1",

doi = "10.1177/0961203308100512",

language = "English",

volume = "18",

pages = "597--607",

journal = "Lupus",

issn = "0961-2033",

publisher = "SAGE Publications",

number = "7",

}

TY - JOUR

T1 - Circulating chromatin-anti-chromatin antibody complexes bind with high affinity to dermo-epidermal structures in murine and human lupus nephritis

AU - Fismen, S

AU - Hedberg, A

AU - Fenton, K A

AU - Jacobsen, S

AU - Krarup, E

AU - Kamper, A L

AU - Rekvig, O P

AU - Mortensen, E S

AU - Fismen, S

AU - Hedberg, A

AU - Fenton, K A

AU - Jacobsen, S

AU - Krarup, E

AU - Kamper, A L

AU - Rekvig, O P

AU - Mortensen, E S

N1 - Keywords: Animals; Antibodies, Anti-Idiotypic; Antigen-Antibody Complex; Biopsy; Capillaries; Cell Adhesion Molecules; Chromatin; Collagen Type I; Collagen Type IV; Disease Models, Animal; Female; Histones; Humans; Immunoglobulin G; Kidney Glomerulus; Lupus Nephritis; Mice; Mice, Inbred MRL lpr; Mice, Inbred NZB; Skin; Transcription Factors

PY - 2009/6/1

Y1 - 2009/6/1

N2 - Murine and human lupus nephritis are characterized by glomerular deposits of electron-dense structures (EDS). Dominant components of EDS are chromatin fragments and IgG antibodies. Whether glomerular EDS predispose for similar deposits in skin is unknown. We analysed (i) whether dermo-epidermal immune complex deposits have similar molecular composition as glomerular deposits, (ii) whether chromatin fragments bind dermo-epidermal structures, and (iii) whether deposits in nephritic glomeruli predispose for accumulation of similar deposits in skin. Paired skin and kidney biopsies from nephritic (NZBxNZW)F1 and MRL-lpr/lpr mice and from five patients with lupus nephritis were analysed by immunofluorescence, immune electron microscopy (IEM) and co-localization TUNEL IEM. Affinity of chromatin fragments for membrane structures was determined by surface plasmon resonance. Results demonstrated (i) presence of EDS containing chromatin fragments and IgG in both organs in nephritic patients, (ii) chromatin fragments possessed high affinity for dermo-epidermal laminins and collagens, (iii) glomerular immune complex deposits did not predict similar interstitial deposits in skin, although such complexes were present in capillary lumina in glomeruli and skin of all nephritic individuals. Thus, chromatin-IgG complexes accounting for lupus nephritis seem to reach skin through circulation, but other undetermined factors are required for these complexes to deposit within skin membranes.

AB - Murine and human lupus nephritis are characterized by glomerular deposits of electron-dense structures (EDS). Dominant components of EDS are chromatin fragments and IgG antibodies. Whether glomerular EDS predispose for similar deposits in skin is unknown. We analysed (i) whether dermo-epidermal immune complex deposits have similar molecular composition as glomerular deposits, (ii) whether chromatin fragments bind dermo-epidermal structures, and (iii) whether deposits in nephritic glomeruli predispose for accumulation of similar deposits in skin. Paired skin and kidney biopsies from nephritic (NZBxNZW)F1 and MRL-lpr/lpr mice and from five patients with lupus nephritis were analysed by immunofluorescence, immune electron microscopy (IEM) and co-localization TUNEL IEM. Affinity of chromatin fragments for membrane structures was determined by surface plasmon resonance. Results demonstrated (i) presence of EDS containing chromatin fragments and IgG in both organs in nephritic patients, (ii) chromatin fragments possessed high affinity for dermo-epidermal laminins and collagens, (iii) glomerular immune complex deposits did not predict similar interstitial deposits in skin, although such complexes were present in capillary lumina in glomeruli and skin of all nephritic individuals. Thus, chromatin-IgG complexes accounting for lupus nephritis seem to reach skin through circulation, but other undetermined factors are required for these complexes to deposit within skin membranes.

U2 - 10.1177/0961203308100512

DO - 10.1177/0961203308100512

M3 - Journal article

C2 - 19433459

SN - 0961-2033

VL - 18

SP - 597

EP - 607

JO - Lupus

JF - Lupus

IS - 7

ER -