Cleaved beta 2-microglobulin partially attains a conformation that has amyloidogenic features.

Niels H H Heegaard; Peter Roepstorff; Steen G Melberg; Mogens H Nissen

doi:10.1074/jbc.M108837200

Cleaved beta 2-microglobulin partially attains a conformation that has amyloidogenic features.

Niels H H Heegaard, Peter Roepstorff, Steen G Melberg, Mogens H Nissen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

50 Citationer (Scopus)

Abstract

Udgivelsesdato: 2002-Mar-29

Originalsprog	Engelsk
Tidsskrift	Journal of Biological Chemistry
Vol/bind	277
Udgave nummer	13
Sider (fra-til)	11184-9
Antal sider	5
ISSN	0021-9258
DOI	https://doi.org/10.1074/jbc.M108837200
Status	Udgivet - 2002

Bibliografisk note

Keywords: Amyloid; Chromatography, High Pressure Liquid; Circular Dichroism; Electrophoresis, Capillary; Humans; Hydrolysis; Mass Spectrometry; Protein Conformation; Renal Dialysis; Uremia; beta 2-Microglobulin

Adgang til dokumentet

10.1074/jbc.M108837200

Citationsformater

@article{1d3e3240b93a11ddae57000ea68e967b,

title = "Cleaved beta 2-microglobulin partially attains a conformation that has amyloidogenic features.",

abstract = "beta(2)-Microglobulin, a small protein localized in serum and on cell surfaces, can adopt specific aggregating conformations that generate amyloid in tissues and joints as a complication to long-term hemodialysis. We characterize a proteolytic variant of beta(2)-microglobulin (cleaved after Lys(58)) that as a trimmed form (Lys(58) is removed) can be demonstrated in the circulation in patients with chronic disease. An unexpected electrophoretic heterogeneity of these two cleaved variants was demonstrated by capillary electrophoresis under physiological conditions. Each separated into a fast and a slow component while appearing homogeneous, except for a fraction of oxidized species detected by other techniques. The two components had different binding affinities for heparin and for the amyloid-specific dye Congo red, and the equilibrium between the two forms was dependent on solvent conditions. Together with analysis of the differences in circular dichroism, the results suggest that beta(2)-microglobulin cleaved after Lys(58) readily adopts two equilibrium conformations under native conditions. In the cleaved and trimmed beta(2)-microglobulin that appears in vivo, the less populated conformation is characterized by an increased affinity for Congo red. These observations may help elucidate why beta(2)-microglobulin polymerizes as amyloid in chronic hemodialysis and facilitate the search for means to inhibit this process.",

author = "Heegaard, {Niels H H} and Peter Roepstorff and Melberg, {Steen G} and Nissen, {Mogens H}",

note = "Keywords: Amyloid; Chromatography, High Pressure Liquid; Circular Dichroism; Electrophoresis, Capillary; Humans; Hydrolysis; Mass Spectrometry; Protein Conformation; Renal Dialysis; Uremia; beta 2-Microglobulin",

year = "2002",

doi = "10.1074/jbc.M108837200",

language = "English",

volume = "277",

pages = "11184--9",

journal = "Journal of Biological Chemistry",

issn = "0021-9258",

publisher = "American Society for Biochemistry and Molecular Biology, Inc.",

number = "13",

}

TY - JOUR

T1 - Cleaved beta 2-microglobulin partially attains a conformation that has amyloidogenic features.

AU - Heegaard, Niels H H

AU - Roepstorff, Peter

AU - Melberg, Steen G

AU - Nissen, Mogens H

N1 - Keywords: Amyloid; Chromatography, High Pressure Liquid; Circular Dichroism; Electrophoresis, Capillary; Humans; Hydrolysis; Mass Spectrometry; Protein Conformation; Renal Dialysis; Uremia; beta 2-Microglobulin

PY - 2002

Y1 - 2002

N2 - beta(2)-Microglobulin, a small protein localized in serum and on cell surfaces, can adopt specific aggregating conformations that generate amyloid in tissues and joints as a complication to long-term hemodialysis. We characterize a proteolytic variant of beta(2)-microglobulin (cleaved after Lys(58)) that as a trimmed form (Lys(58) is removed) can be demonstrated in the circulation in patients with chronic disease. An unexpected electrophoretic heterogeneity of these two cleaved variants was demonstrated by capillary electrophoresis under physiological conditions. Each separated into a fast and a slow component while appearing homogeneous, except for a fraction of oxidized species detected by other techniques. The two components had different binding affinities for heparin and for the amyloid-specific dye Congo red, and the equilibrium between the two forms was dependent on solvent conditions. Together with analysis of the differences in circular dichroism, the results suggest that beta(2)-microglobulin cleaved after Lys(58) readily adopts two equilibrium conformations under native conditions. In the cleaved and trimmed beta(2)-microglobulin that appears in vivo, the less populated conformation is characterized by an increased affinity for Congo red. These observations may help elucidate why beta(2)-microglobulin polymerizes as amyloid in chronic hemodialysis and facilitate the search for means to inhibit this process.

AB - beta(2)-Microglobulin, a small protein localized in serum and on cell surfaces, can adopt specific aggregating conformations that generate amyloid in tissues and joints as a complication to long-term hemodialysis. We characterize a proteolytic variant of beta(2)-microglobulin (cleaved after Lys(58)) that as a trimmed form (Lys(58) is removed) can be demonstrated in the circulation in patients with chronic disease. An unexpected electrophoretic heterogeneity of these two cleaved variants was demonstrated by capillary electrophoresis under physiological conditions. Each separated into a fast and a slow component while appearing homogeneous, except for a fraction of oxidized species detected by other techniques. The two components had different binding affinities for heparin and for the amyloid-specific dye Congo red, and the equilibrium between the two forms was dependent on solvent conditions. Together with analysis of the differences in circular dichroism, the results suggest that beta(2)-microglobulin cleaved after Lys(58) readily adopts two equilibrium conformations under native conditions. In the cleaved and trimmed beta(2)-microglobulin that appears in vivo, the less populated conformation is characterized by an increased affinity for Congo red. These observations may help elucidate why beta(2)-microglobulin polymerizes as amyloid in chronic hemodialysis and facilitate the search for means to inhibit this process.

U2 - 10.1074/jbc.M108837200

DO - 10.1074/jbc.M108837200

M3 - Journal article

C2 - 11801591

SN - 0021-9258

VL - 277

SP - 11184

EP - 11189

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 13

ER -