Abstract
The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.
Originalsprog | Engelsk |
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Tidsskrift | Research in Microbiology |
Vol/bind | 164 |
Udgave nummer | 3 |
Sider (fra-til) | 205-210 |
Antal sider | 6 |
ISSN | 0923-2508 |
DOI | |
Status | Udgivet - 2013 |
Udgivet eksternt | Ja |
Emneord
- Det tidligere LIFE
- Klebsiella pneumoniae
- Clp ATPase
- ClpK
- Heat resistance
- ClpP
- sHSP