ClpP-dependent and -independent activities encoded by the polycistronic clpK-encoding locus contribute to heat shock survival in Klebsiella pneumoniae

Martin Saxtorph Bojer, Carsten Struve, Hanne Ingmer, Karen Angeliki Krogfelt

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

12 Citationer (Scopus)

Abstract

The family of Clp ATPases plays an important role in bacterial physiology. Here we characterize the genetic locus encompassing a newly described plasmid-encoded ClpK protein protecting Klebsiella pneumoniae cells during heat shock. We demonstrate that the clpK gene is located in a polycistronic operon and that the variable downstream gene content correlates with heat-resistant phenotypes of different isolates. ClpK is encoded by a multifunctional transcriptional unit characterized by both ClpP-dependent and -independent activities. Notably, our data show that ClpP is indispensible for thermoprotection exerted by ClpK alone, suggesting that ClpK is a new member of the family of ClpP-interacting Clp ATPases.
OriginalsprogEngelsk
TidsskriftResearch in Microbiology
Vol/bind164
Udgave nummer3
Sider (fra-til)205-210
Antal sider6
ISSN0923-2508
DOI
StatusUdgivet - 2013
Udgivet eksterntJa

Emneord

  • Det tidligere LIFE
  • Klebsiella pneumoniae
  • Clp ATPase
  • ClpK
  • Heat resistance
  • ClpP
  • sHSP

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