CNOT6: A Novel Regulator of DNA Mismatch Repair

Peng Song, Shaojun Liu, Dekang Liu, Guido Keijzers, Daniela Bakula, Shunlei Duan, Niels de Wind, Zilu Ye, Sergey Y. Vakhrushev, Morten Scheibye-Knudsen, Lene Juel Rasmussen*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

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Abstract

DNA mismatch repair (MMR) is a highly conserved pathway that corrects both base–base mispairs and insertion-deletion loops (IDLs) generated during DNA replication. Defects in MMR have been linked to carcinogenesis and drug resistance. However, the regulation of MMR is poorly understood. Interestingly, CNOT6 is one of four deadenylase subunits in the conserved CCR4-NOT complex and it targets poly(A) tails of mRNAs for degradation. CNOT6 is overexpressed in acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML) and androgen-independent prostate cancer cells, which suggests that an altered expression of CNOT6 may play a role in tumorigenesis. Here, we report that a depletion of CNOT6 sensitizes human U2OS cells to N-methyl-N′nitro-N-nitrosoguanidine (MNNG) and leads to enhanced apoptosis. We also demonstrate that the depletion of CNOT6 upregulates MMR and decreases the mutation frequency in MMR-proficient cells. Furthermore, the depletion of CNOT6 increases the stability of mRNA transcripts from MMR genes, leading to the increased expression of MMR proteins. Our work provides insight into a novel CNOT6-dependent mechanism for regulating MMR.

OriginalsprogEngelsk
Artikelnummer521
TidsskriftCells
Vol/bind11
Udgave nummer3
ISSN2073-4409
DOI
StatusUdgivet - 2022

Bibliografisk note

Funding Information:
This research was funded by the Nordea-fonden, Olav Thon Foundation, Sven Wewers fond, and Novo Nordisk Foundation [NNF17OC0027812]; China Scholarship Council, China. D.L. was funded by the grant from Chinese National Natural Science Foundation [31800682]. The Copenhagen Center for Glycomics including S.Y.V. and Z.Y. are supported by a Danish National Research Foundation Grant (DNRF107).

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

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