Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation

Fons Bonekamp; Henrik Dalbøge Andersen; Torkild Christensen; Kaj Frank Jensen

Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation

Fons Bonekamp, Henrik Dalbøge Andersen, Torkild Christensen, Kaj Frank Jensen

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

68 Citationer (Scopus)

Abstract

Udgivelsesdato: 1985 June 11

Originalsprog	Engelsk
Tidsskrift	Nucleic Acids Research
Vol/bind	13
Udgave nummer	11
Sider (fra-til)	4113-4123
ISSN	0305-1048
Status	Udgivet - 1985

Adgang til dokumentet

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC341299/pdf/nar00305-0327.pdf

Citationsformater

Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation. / Bonekamp, Fons; Andersen, Henrik Dalbøge; Christensen, Torkild; Jensen, Kaj Frank.

I: Nucleic Acids Research, Bind 13, Nr. 11, 1985, s. 4113-4123.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

@article{d5efa550841e11dcbee902004c4f4f50,

title = "Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation",

abstract = "This communication describes an assay for the relative translation efficiency of individual codons which makes use of the pyrE attenuator to probe the coupling between transcription and translation at the end of an artificial leader peptide. By cloning of short synthetic DNA fragments the codons to be tested were placed in the middle of the leader peptide and the downstream transcription of a pyrE{"}lacZ gene was monitored by measuring beta-galactosidase activity. The substitution, one by one, of three AGG codons for arginine with three CGT codons for the same amino acid residue was found to cause a two fold increase per codon of transcription over the pyrE attenuator, such that an eight fold higher frequency of pyrE expression was seen when all three AGG codons were replaced by CGT codons. No such effect of codon composition was observed, when the cells were grown with a low UTP pool which causes a reduction of the mRNA chain growth rate.",

author = "Fons Bonekamp and Andersen, {Henrik Dalb{\o}ge} and Torkild Christensen and Jensen, {Kaj Frank}",

year = "1985",

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journal = "Nucleic Acids Research",

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T1 - Codon-defined ribosomal pausing in Escherichia coli detected by using the pyrE attenuator to probe the coupling between transcription and translation

AU - Bonekamp, Fons

AU - Andersen, Henrik Dalbøge

AU - Christensen, Torkild

AU - Jensen, Kaj Frank

PY - 1985

Y1 - 1985

N2 - This communication describes an assay for the relative translation efficiency of individual codons which makes use of the pyrE attenuator to probe the coupling between transcription and translation at the end of an artificial leader peptide. By cloning of short synthetic DNA fragments the codons to be tested were placed in the middle of the leader peptide and the downstream transcription of a pyrE"lacZ gene was monitored by measuring beta-galactosidase activity. The substitution, one by one, of three AGG codons for arginine with three CGT codons for the same amino acid residue was found to cause a two fold increase per codon of transcription over the pyrE attenuator, such that an eight fold higher frequency of pyrE expression was seen when all three AGG codons were replaced by CGT codons. No such effect of codon composition was observed, when the cells were grown with a low UTP pool which causes a reduction of the mRNA chain growth rate.

AB - This communication describes an assay for the relative translation efficiency of individual codons which makes use of the pyrE attenuator to probe the coupling between transcription and translation at the end of an artificial leader peptide. By cloning of short synthetic DNA fragments the codons to be tested were placed in the middle of the leader peptide and the downstream transcription of a pyrE"lacZ gene was monitored by measuring beta-galactosidase activity. The substitution, one by one, of three AGG codons for arginine with three CGT codons for the same amino acid residue was found to cause a two fold increase per codon of transcription over the pyrE attenuator, such that an eight fold higher frequency of pyrE expression was seen when all three AGG codons were replaced by CGT codons. No such effect of codon composition was observed, when the cells were grown with a low UTP pool which causes a reduction of the mRNA chain growth rate.

M3 - Journal article

VL - 13

SP - 4113

EP - 4123

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 11

ER -