Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism

Matthew D. Lynes; Farnaz Shamsi; Elahu Gosney Sustarsic; Luiz O. Leiria; Chih Hao Wang; Sheng Chiang Su; Tian Lian Huang; Fei Gao; Niven R. Narain; Emily Y. Chen; Aaron M. Cypess; Tim J. Schulz; Zachary Gerhart-Hines; Michael A. Kiebish; Yu Hua Tseng

doi:10.1016/j.celrep.2018.06.073

Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism

Matthew D. Lynes
, Farnaz Shamsi
, Elahu Gosney Sustarsic
, Luiz O. Leiria
, Chih Hao Wang
, Sheng Chiang Su
, Tian Lian Huang
, Fei Gao
, Niven R. Narain
, Emily Y. Chen
, Aaron M. Cypess
, Tim J. Schulz
, Zachary Gerhart-Hines
, Michael A. Kiebish
, Yu Hua Tseng^*

^*Corresponding author af dette arbejde

Gerhart-Hines Group

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review

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Abstract

Thermogenic fat expends energy during cold for temperature homeostasis, and its activity regulates nutrient metabolism and insulin sensitivity. We measured cold-activated lipid landscapes in circulation and in adipose tissue by MS/MS^ALL shotgun lipidomics. We created an interactive online viewer to visualize the changes of specific lipid species in response to cold. In adipose tissue, among the approximately 1,600 lipid species profiled, we identified the biosynthetic pathway of the mitochondrial phospholipid cardiolipin as coordinately activated in brown and beige fat by cold in wild-type and transgenic mice with enhanced browning of white fat. Together, these data provide a comprehensive lipid bio-signature of thermogenic fat activation in circulation and tissue and suggest pathways regulated by cold exposure. In this resource, Lynes et al. use a sensitive MS/MS^ALL technique to profile over 1,000 lipid species in the blood and adipose tissue of humans and mice exposed to cold. Using the online viewer they created to view these data, pathways activated by cold exposure can be identified.

Originalsprog	Engelsk
Tidsskrift	Cell Reports
Vol/bind	24
Udgave nummer	3
Sider (fra-til)	781-790
Antal sider	10
ISSN	2211-1247
DOI	https://doi.org/10.1016/j.celrep.2018.06.073
Status	Udgivet - 2018

Finansiering

This work was supported in part by U.S. NIH grants R01DK077097 and R01DK102898 (to Y.-H.T.) and by grant P30DK036836 (to Joslin Diabetes Center’s Diabetes Research Center; DRC) from the National Institute of Diabetes and Digestive and Kidney Diseases . M.D.L. was supported by NIH grants T32DK007260 , F32DK102320 , and K01DK111714 . We thank H. Pan in the Joslin Bioinformatics Core. This manuscript is dedicated to the memory of Sean Peters.

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Citationsformater

Lynes, M. D., Shamsi, F., Sustarsic, E. G., Leiria, L. O., Wang, C. H., Su, S. C., Huang, T. L., Gao, F., Narain, N. R., Chen, E. Y., Cypess, A. M., Schulz, T. J., Gerhart-Hines, Z., Kiebish, M. A., & Tseng, Y. H. (2018). Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism. Cell Reports, 24(3), 781-790. https://doi.org/10.1016/j.celrep.2018.06.073

Lynes, MD, Shamsi, F, Sustarsic, EG, Leiria, LO, Wang, CH, Su, SC, Huang, TL, Gao, F, Narain, NR, Chen, EY, Cypess, AM, Schulz, TJ, Gerhart-Hines, Z, Kiebish, MA & Tseng, YH 2018, 'Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism', Cell Reports, bind 24, nr. 3, s. 781-790. https://doi.org/10.1016/j.celrep.2018.06.073

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title = "Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism",

abstract = "Thermogenic fat expends energy during cold for temperature homeostasis, and its activity regulates nutrient metabolism and insulin sensitivity. We measured cold-activated lipid landscapes in circulation and in adipose tissue by MS/MSALL shotgun lipidomics. We created an interactive online viewer to visualize the changes of specific lipid species in response to cold. In adipose tissue, among the approximately 1,600 lipid species profiled, we identified the biosynthetic pathway of the mitochondrial phospholipid cardiolipin as coordinately activated in brown and beige fat by cold in wild-type and transgenic mice with enhanced browning of white fat. Together, these data provide a comprehensive lipid bio-signature of thermogenic fat activation in circulation and tissue and suggest pathways regulated by cold exposure. In this resource, Lynes et al. use a sensitive MS/MSALL technique to profile over 1,000 lipid species in the blood and adipose tissue of humans and mice exposed to cold. Using the online viewer they created to view these data, pathways activated by cold exposure can be identified.",

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T1 - Cold-Activated Lipid Dynamics in Adipose Tissue Highlights a Role for Cardiolipin in Thermogenic Metabolism

AU - Lynes, Matthew D.

AU - Shamsi, Farnaz

AU - Sustarsic, Elahu Gosney

AU - Leiria, Luiz O.

AU - Wang, Chih Hao

AU - Su, Sheng Chiang

AU - Huang, Tian Lian

AU - Gao, Fei

AU - Narain, Niven R.

AU - Chen, Emily Y.

AU - Cypess, Aaron M.

AU - Schulz, Tim J.

AU - Gerhart-Hines, Zachary

AU - Kiebish, Michael A.

AU - Tseng, Yu Hua

PY - 2018

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N2 - Thermogenic fat expends energy during cold for temperature homeostasis, and its activity regulates nutrient metabolism and insulin sensitivity. We measured cold-activated lipid landscapes in circulation and in adipose tissue by MS/MSALL shotgun lipidomics. We created an interactive online viewer to visualize the changes of specific lipid species in response to cold. In adipose tissue, among the approximately 1,600 lipid species profiled, we identified the biosynthetic pathway of the mitochondrial phospholipid cardiolipin as coordinately activated in brown and beige fat by cold in wild-type and transgenic mice with enhanced browning of white fat. Together, these data provide a comprehensive lipid bio-signature of thermogenic fat activation in circulation and tissue and suggest pathways regulated by cold exposure. In this resource, Lynes et al. use a sensitive MS/MSALL technique to profile over 1,000 lipid species in the blood and adipose tissue of humans and mice exposed to cold. Using the online viewer they created to view these data, pathways activated by cold exposure can be identified.

AB - Thermogenic fat expends energy during cold for temperature homeostasis, and its activity regulates nutrient metabolism and insulin sensitivity. We measured cold-activated lipid landscapes in circulation and in adipose tissue by MS/MSALL shotgun lipidomics. We created an interactive online viewer to visualize the changes of specific lipid species in response to cold. In adipose tissue, among the approximately 1,600 lipid species profiled, we identified the biosynthetic pathway of the mitochondrial phospholipid cardiolipin as coordinately activated in brown and beige fat by cold in wild-type and transgenic mice with enhanced browning of white fat. Together, these data provide a comprehensive lipid bio-signature of thermogenic fat activation in circulation and tissue and suggest pathways regulated by cold exposure. In this resource, Lynes et al. use a sensitive MS/MSALL technique to profile over 1,000 lipid species in the blood and adipose tissue of humans and mice exposed to cold. Using the online viewer they created to view these data, pathways activated by cold exposure can be identified.

KW - brown adipose tissue

KW - cardiolipin

KW - lipidomics

KW - thermogenesis

KW - white adipose tissue

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DO - 10.1016/j.celrep.2018.06.073

M3 - Journal article

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