@article{fc59b0b074c411dbbee902004c4f4f50,
title = "Combined Triplex/Duplex Invasion of Double-Stranded DNA by {"}Tail-Clamp{"} Peptide Nucleic Acid",
abstract = "{"}Tail-clamp{"} PNAs composed of a short (hexamer) homopyrimidine triplex forming domain and a (decamer) mixed sequence duplex forming extension have been designed. Tail-clamp PNAs display significantly increased binding to single-stranded DNA compared with PNAs lacking a duplex-forming extension as determined by T-m measurements. Binding to double-stranded (ds) DNA occurred by combined triplex and duplex invasion as analyzed by permanganate probing. Furthermore, C-50 measurements revealed that tail-clamp PNAs consistently bound the dsDNA target more efficiently, and kinetics experiments revealed that this was due to a dramatically reduced dissociation rate of such complexes. Increasing the PNA net charge also increased binding efficiency, but unexpectedly, this increase was much more pronounced for tailless-clamp PNAs than for tail-clamp PNAs. Finally, shortening the tail-clamp PNA triplex invasion moiety to five residues was feasible, but four bases were not sufficient to yield detectable dsDNA binding. The results validate the tail-clamp PNA concept and expand the applications of the P-loop technology.",
author = "Thomas Bentin and Larsen, \{H. J.\} and Nielsen, \{Peter E.\}",
year = "2003",
doi = "10.1021/bi0351918",
language = "English",
volume = "42",
pages = "13987--13995",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "47",
}